Wolbachia bacteria suppress A

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Wolbachia bacteria suppress A Wolbachia bacteria suppress A. pasteurianus across various life stages of D. melanogaster. Wolbachia bacteria suppress A. pasteurianus across various life stages of D. melanogaster. (A) Schematic of the experimental setup used. Stopwatches indicate sample collection times. (B to E) PCR products obtained with A. pasteurianus and L. plantarum species-specific primers from BstZ17I-digested total genomic DNA from wMel-free (W−) or -infected D. melanogaster. (B) The parental flies are F0 0- to 7-day-old males and females. (C to E) Experiments were done in triplicate, and each sample consisted of five adults (B, D, E) or five larvae (C). Vial numbers are above the gel lanes. (C) F1 unsexed L3 larvae (n = 3), (D) F1 0- to 7-day-old male and female flies (n = 3). (E) F1 7- to 14-day-old male and female flies (n = 3). Rama K. Simhadri et al. mSphere 2017; doi:10.1128/mSphere.00287-17