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Volume 63, Issue 6, Pages 2152-2161 (June 2003) Reduced tubular cation transport in diabetes: Prevented by ACE inhibition Merlin C. Thomas, Chris Tikellis, Wendy C. Burns, Vicki Thallas, Josephine M. Forbes, Zemin Cao, Tanya M. Osicka, Leileata M. Russo, George Jerums, Hany Ghabrial, Mark E. Cooper, Phillip Kantharidis Kidney International Volume 63, Issue 6, Pages 2152-2161 (June 2003) DOI: 10.1046/j.1523-1755.2003.00006.x Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 1 Microarray cDNA spots. Reduced expression of organic cation transporters (OCT)-1 (27%) (left panel) and OCT-2 (48%) (middle panel) mRNA is shown in animals with diabetes (pooled samples, N = 10) compared to control (N = 10). A 50% reduction in the expression of glutathione peroxidase 3(GPX-3) (right panel) in diabetic kidney samples is shown as a marker of tubular injury. Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 2 Expression of mRNA. (A) Expression is shown for organic cation transporters (OCT)-1 (left panel), OCT-2 (middle panel), and glutathione peroxidase 3 (GPX-3) (right panel) in control animals (upper panel) and experimental diabetes (lower panel). (B) Expression is shown for OCT-1 (left panel), OCT-2 (middle panel), and GPX-3 (right panel) in control animals (upper panel) and experimental diabetes (lower panel). Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 3 Renal expression of organic cation transporters (OCT)-1 mRNA (A) and OCT-2 (B) measured using real-time reverse transcription-polymerase chain reaction (RT-PCR) in control animals (□), diabetes (■), and diabetes treated with ramipril (). Mean + SEM. *Control vs. diabetes, P < 0.05; #Diabetes vs. diabetes + ramipril, P < 0.05. Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 4 Localization of renal expression. Expression of organic cation transporters (OCT)-1 protein (left panel), OCT-2 (middle panel), and osteopontin (right panel) in control animals (top row), diabetes (second row), diabetes treated with ramipril (third row), and angiotensin-infused animal (bottom row). Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 5 Immunoblot showing expression. Expression of organic cation transporters (OCT)-1 protein (A), OCT-2 (B), and osteopontin (C) in control animals (left panel), diabetes (middle panel), and diabetes treated with ramipril (right panel). Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 6 Tubular clearance of N-methyl-nicotinamide/glomerular filtration rate (NMN/GFR) in control animals (□), diabetes (•), and diabetes treated with ramipril (▵). Kidney International 2003 63, 2152-2161DOI: (10.1046/j.1523-1755.2003.00006.x) Copyright © 2003 International Society of Nephrology Terms and Conditions