Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies H. Pereira, S.G. Caridade,

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Biomechanical and cellular segmental characterization of human meniscus: building the basis for Tissue Engineering therapies H. Pereira, S.G. Caridade, A.M. Frias, J. Silva-Correia, D.R. Pereira, I.F. Cengiz, J.F. Mano, J.M. Oliveira, J. Espregueira-Mendes, R.L. Reis Osteoarthritis and Cartilage Volume 22, Issue 9, Pages 1271-1281 (September 2014) DOI: 10.1016/j.joca.2014.07.001 Copyright © 2014 Terms and Conditions

Fig. 1 Lateral meniscus obtained from live donor that undergone a TKA for unicompartmental OA (A), meniscus division in anterior, mid body and posterior segments (B), and harvesting of 4 mm cylinders (C). Osteoarthritis and Cartilage 2014 22, 1271-1281DOI: (10.1016/j.joca.2014.07.001) Copyright © 2014 Terms and Conditions

Fig. 2 DMA of fresh lateral and medial meniscus studied at several frequencies (mean with 95% confidence interval (CI), n = 108 samples from 15 donors): segmental evaluation of E′ values for lateral (A) and medial menisci (B); segmental evaluation of E′′ values for lateral (C), and medial (D) menisci; and segmental evaluation of tan δ for lateral (E) and medial (F) menisci. Numeric data are presented on Table 2s of Supplementary data. Osteoarthritis and Cartilage 2014 22, 1271-1281DOI: (10.1016/j.joca.2014.07.001) Copyright © 2014 Terms and Conditions

Fig. 3 3D reconstruction from micro-CT images of freeze-dried lateral meniscus of left knee (A); 2D micro-CT images of a part of freeze-dried medial meniscus of left knee (B, C), change of porosity (%) across the menisci from the bottom part (tibial surface) to top part (femoral surface) (D). Osteoarthritis and Cartilage 2014 22, 1271-1281DOI: (10.1016/j.joca.2014.07.001) Copyright © 2014 Terms and Conditions

Fig. 4 Microscopy image of the HMC's in culture after isolation using an enzymatic digestion method. It is possible to observe meniscus cells depicting rounded and fusiform-like morphologies (A). The mean value (n = 5 patients) of percentage of positive cells for markers CD73, CD90, and CD105, is 97.2 (95.6–98.9)%, and for the marker CD44 the mean percentage value is 96.7 (95.1–98.2)%. The same densities were also analyzed for the expression of CD31, CD34 and CD45, and the obtained mean percentage values are 2.1 (1.8–2.4)%, 3.1 (2.9–3.3)% and 0.2 (0.1–0.2)%, respectively. Besides, two different passages (P2, P7) were analyzed (n = 2); and it was found that the analyzed markers and their percentages of expression were maintained. Osteoarthritis and Cartilage 2014 22, 1271-1281DOI: (10.1016/j.joca.2014.07.001) Copyright © 2014 Terms and Conditions