Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR D. Tarragó, A. Fenoll, D. Sánchez-Tatay, L.A.

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Presentation transcript:

Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR D. Tarragó, A. Fenoll, D. Sánchez-Tatay, L.A. Arroyo, C. Muñoz-Almagro, C. Esteva, W.P. Hausdorff, J. Casal, I. Obando Clinical Microbiology and Infection Volume 14, Issue 9, Pages 828-834 (September 2008) DOI: 10.1111/j.1469-0691.2008.02028.x Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Diagrammatic representation of the molecular analysis of 88 parapneumonic empyema cases without microbiological pneumococcal isolates. Eighty-eight samples were tested for pneumococcal DNA by ply PCR and wzg real-time PCR. Twenty-one pleural fluid samples were not tested by serotype real-time PCR because they were negative according to both ply PCR and wzg real-time PCR, they did not contain pneumococci, or there was an insufficient amount of sample. Subsequently, 67 samples were analyzed by serotype real-time PCR, 52 of which were identified to the serotype level. Clinical Microbiology and Infection 2008 14, 828-834DOI: (10.1111/j.1469-0691.2008.02028.x) Copyright © 2008 European Society of Clinical Infectious Diseases Terms and Conditions