Evaluation of quantitative PCR for early diagnosis of Pseudomonas aeruginosa infection in cystic fibrosis: a prospective cohort study G. Héry-Arnaud,

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Evaluation of quantitative PCR for early diagnosis of Pseudomonas aeruginosa infection in cystic fibrosis: a prospective cohort study G. Héry-Arnaud, E. Nowak, J. Caillon, V. David, A. Dirou, K. Revert, M.-R. Munck, I. Frachon, A. Haloun, D. Horeau-Langlard, J. Le Bihan, I. Danner-Boucher, S. Ramel, M.-P. Pelletier, S. Rosec, S. Gouriou, E. Poulhazan, C. Payan, C. Férec, G. Rault, G. Le Gal, R. Le Berre Clinical Microbiology and Infection Volume 23, Issue 3, Pages 203-207 (March 2017) DOI: 10.1016/j.cmi.2016.11.016 Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Flowchart of study participants, with culture and quantitative PCR (qPCR) results for the 707 sputum samples. Results of oprL qPCR combined with gyrB/ecfX qPCR if bacterial density on oprL qPCR was ≥730 CFU/mL are reported. Clinical Microbiology and Infection 2017 23, 203-207DOI: (10.1016/j.cmi.2016.11.016) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Kaplan–Meier plot of time to Pseudomonas aeruginosa-positive culture for patients who were quantitative PCR (qPCR) positive according to ‘free’ or ‘never’ status. (qPCR+: oprL qPCR+ combined with gyrB/ecfX qPCR+ if bacterial density on oprL qPCR ≥730 CFU/mL). Clinical Microbiology and Infection 2017 23, 203-207DOI: (10.1016/j.cmi.2016.11.016) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions