Isoflurane-induced myocardial preconditioning is dependent on phosphatidylinositol-3- kinase/Akt signalling J. Raphael, J. Rivo, Y. Gozal British Journal.

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Presentation transcript:

Isoflurane-induced myocardial preconditioning is dependent on phosphatidylinositol-3- kinase/Akt signalling J. Raphael, J. Rivo, Y. Gozal British Journal of Anaesthesia Volume 95, Issue 6, Pages 756-763 (December 2005) DOI: 10.1093/bja/aei264 Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 1 Diagram of the experimental protocol. Animals were subjected to 40 min of regional myocardial ischaemia and 120 min of reperfusion. One minimal alveolar concentration of isoflurane was administered for 30 min followed by 30 min of washout before coronary occlusion. Wortmannin (0.6 mg kg−1, i.v.) was administered 10 min before isoflurane preconditioning. I/R, ischaemia–reperfusion; Iso, isoflurane; Wort+I/R, wortmannin+ischaemia–reperfusion; Wort+Iso, Wortmannin+isoflurane. British Journal of Anaesthesia 2005 95, 756-763DOI: (10.1093/bja/aei264) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 2 CK-MB concentrations. Serum CK-MB concentrations were analysed by an enzymatic assay using a CK assay kit. The increase in CK-MB concentrations was lower in rabbits pretreated with isoflurane than in controls (I/R group). Wortmannin abolished this protective effect and the increase in CK-MB was similar to that seen in the control group. Data are mean and sem. ISCH, ischaemia; REP, reperfusion; I/R, ischaemia–reperfusion; Iso, isoflurane; Wort+I/R, wortmannin+ischaemia–reperfusion; Wort+Iso, wortmannin+isoflurane. *P<0.05 vs I/R; #P<0.05 vs Wort+Iso. British Journal of Anaesthesia 2005 95, 756-763DOI: (10.1093/bja/aei264) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 3 Effects of isoflurane preconditioning with and without wortmannin administration on infarct size. Bar graph shows infarct size as percentage of the area at risk. Isoflurane significantly decreased infarct size compared with control (I/R) animals, whereas wortmannin abolished this protective effect and infarct size was similar to that of the controls. Data are mean and sem. I/R, ischaemia–reperfusion; Iso, isoflurane; Wort+I/R, wortmannin+ischaemia–reperfusion; Wort+Iso, wortmannin+isoflurane. *P<0.05 vs I/R group; #P<0.05 vs Wort+Iso. British Journal of Anaesthesia 2005 95, 756-763DOI: (10.1093/bja/aei264) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions

Fig 4 Effects of isoflurane with and without administration of wortmannin on Akt phosphorylation. (a) Representative western blot analysis of total Akt (top lanes) and phosphorylation of Akt at Ser473 (middle lanes). In each lane the protein concentration was 50 μg. β-Actin (lower lanes) was used to demonstrate equal protein loading. (b) Graphic presentation of the phospho-Akt quantified by integrating the volume of autoradiograms from three separate experiments. Values in the graph are expressed as fold changes over the control and are presented as mean and sem. I/R, ischaemia–reperfusion; Iso, isoflurane; Wort+I/R, wortmannin+ischaemia–reperfusion; Wort+Iso, wortmannin+isoflurane. n=5 in each group. *P<0.05 vs sham-operated group. British Journal of Anaesthesia 2005 95, 756-763DOI: (10.1093/bja/aei264) Copyright © 2005 British Journal of Anaesthesia Terms and Conditions