Ken B. Waites, Li Xiao, Vanya Paralanov, Rose M. Viscardi, John I

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Presentation transcript:

Molecular Methods for the Detection of Mycoplasma and Ureaplasma Infections in Humans Ken B. Waites, Li Xiao, Vanya Paralanov, Rose M. Viscardi, John I. Glass The Journal of Molecular Diagnostics Volume 14, Issue 5, Pages 437-450 (September 2012) DOI: 10.1016/j.jmoldx.2012.06.001 Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Real-time PCR detection of macrolide-resistant M. pneumoniae in clinical samples. Genomic DNA samples of two patients containing the A2063G mutation, verified by sequencing, are purified and tested together with a wild-type (WT) control (M. pneumoniae strain M129, ATCC number 29342). Melting curves (A and B) and corresponding melting peaks (C and D) are shown. A2063/A2064 mutations are analyzed in channel 610 (A and C). The WT melting peak is 67.31°C, whereas the melting temperature (Tm) of A2063G mutants is 63.25°C ± 0.04°C. Thus, a 4°C difference between WT and mutant is observed. The C2617 assay is shown in channel 705 (B and D). Because all samples do not have mutations at this position, they show similar WT Tm values of approximately 68°C, as predicted. Data are reproduced from Xiao et al,34 with permission of Walters Kluwer Health (copyright 2009). The Journal of Molecular Diagnostics 2012 14, 437-450DOI: (10.1016/j.jmoldx.2012.06.001) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions