Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

Slides:

Advertisements

Similar presentations

Intracellular EBV‐encoded LMP1 localizes to late endosomes.

Advertisements

Co‐localization of recombination proteins with double‐strand breaks.

MOSPD2 is an ER‐resident protein able to tether synthetic vesicles by binding the FFAT motif MOSPD2 is an ER‐resident protein able to tether synthetic.

Colocalization of the human trimethylguanosine synthase 1 and survival of motor neuron proteins. Colocalization of the human trimethylguanosine synthase.

DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic proteins. DNA‐anchored LacI–CFP–Luciferase aggregates capture misfolded cytosolic.

REF and ATP5b status on mitochondrial fraction.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Periodic changes in distribution of ERK–GFP fusion protein in cells after stimulation with EGF. (A) Confocal image of cells expressing ERK–GFP both before.

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

Matrix pH elevations propagate along connected, but not fused, mitochondria. Matrix pH elevations propagate along connected, but not fused, mitochondria.

Rab22A regulates melanocyte pigmentation, cargo transport and functions upstream of BLOC‐1 Rab22A regulates melanocyte pigmentation, cargo transport and.

MOSPD2 recruitment to interorganelle contact sites depends on the interaction between the FFAT motif and the MSP domain MOSPD2 recruitment to interorganelle.

Subcellular localization of full‐length and cleaved PINK1 PINK1 immunoblot of cytoplasmic and mitochondrial fractions from HeLa cells transfected with.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

c‐Abl acetylation on Lys 730 promotes myogenic differentiation.

Non‐specific activation of gene transcription by exogenous Fe65 in luciferase‐based reporter assays. Non‐specific activation of gene transcription by exogenous.

NM1 localizes in nucleoli and is required for Pol I transcription.

Nore1 enhances interaction between HIPK1 and Mdm2.

Interactions between Aiolos and Ikaros proteins are detected within the cell nucleus. Interactions between Aiolos and Ikaros proteins are detected within.

NoRC regulates telomeric heterochromatin.

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

Dynamics of cell–cell repulsion regulated by PYK2 localized at focal adhesions in migrating cells. Dynamics of cell–cell repulsion regulated by PYK2 localized.

Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that compromise NF‐κB activation. Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that.

Mitochondrial targeting of ceramide induces lethal mitophagy in HPV(+) cells Mitochondrial targeting of ceramide induces lethal mitophagy in HPV(+) cells.

VAP‐A is recruited to interorganelle contact sites together with MOSPD2 VAP‐A is recruited to interorganelle contact sites together with MOSPD2 AHeLa cells.

Abnormal accumulation of U snRNPs in motor neuron nuclei of ALS patients.A–D.Immunofluorescent staining of TDP‐43 and U snRNPs using an anti‐Sm proteins.

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

Identification of MOSPD2, a new FFAT motif‐binding protein

The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL.

The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)‏ The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)

The MSP domain of MOSPD2 binds the FFAT motif

MOSPD2 is a putative tail‐anchored protein

Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.

Rab22A localizes to the E/SE and REs and regulates RE dynamics

Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.

Innervation of Wt1+ neurons and number of commissural neurons in neonatal mice. Innervation of Wt1+ neurons and number of commissural neurons in neonatal.

Topisirovic et al., (2003) EMBO J,22:

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL.

FFAT‐containing proteins recruit the ER‐resident MOSPD2 protein to interorganelle contact sites FFAT‐containing proteins recruit the ER‐resident MOSPD2.

The MSP domain of MOSPD2 binds the FFAT motif with an affinity in the micromolar range The MSP domain of MOSPD2 binds the FFAT motif with an affinity in.

Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on.

Characterization of cholesterol‐enriched endosomes in HeLa/STARD3 cells Characterization of cholesterol‐enriched endosomes in HeLa/STARD3 cells HeLa/STARD3.

STARD3 favors cholesterol accumulation in endosomes

The ER is the main source of cholesterol accumulated by STARD3 in endosomes The ER is the main source of cholesterol accumulated by STARD3 in endosomes.

MOSPD2 silencing affects interorganelle contact sites organization

Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

BDNF expression in the cerebellum and brain stem region.

Fmrp regulates E‐cadherin and Vimentin in breast cancer cells Fmrp (red) and E‐cadherin (green) detection by I.F. in 4T1 cells expressing different Fmrp.

MOSPD2 tethers the ER to organelles bearing FFAT‐containing proteins

STARD3‐mediated cholesterol accumulation in endosomes occurs at the expense of plasma membrane STARD3‐mediated cholesterol accumulation in endosomes occurs.

Colocalization of microinjected miRNAs with PBs and cognate mRNA targets. Colocalization of microinjected miRNAs with PBs and cognate mRNA targets. (A)

Visualization of Oleic Acid-induced Transdermal Diffusion Pathways Using Two-photon Fluorescence Microscopy Betty Yu, Robert Langer, Daniel Blankschtein

Loss of tight junctions on Rac1 activation in mature cysts.

Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs.

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation in endosomes VAP protein knockdown abolishes STARD3‐mediated cholesterol accumulation.

The CHCH domain is necessary for mitochondrial import of CHCHD10

Ubqln1 interacts with Ubqln4.

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

AS aggregates interact with SERCA in SH‐SY5Y cells

Peri‐nuclear Rab34‐positive membranes contact lysosomes

Tangle formation and downregulation of miR‐132‐3p in LOAD A‐G

Figure 3 ADAM22 mutant proteins do not bind to LGI1

A highly conserved six-amino-acid region in the C-terminal CT domain of MARCH8 is responsible for its ability to downregulate TfR. A highly conserved six-amino-acid.

Changes in fibroblast activation and proliferation outside the wound and CHP staining in the wound bed (related to Fig 5)‏ Changes in fibroblast activation.

The effects of a dominant negative mutant of lamin B1 on lamin distribution in HeLa cells. The effects of a dominant negative mutant of lamin B1 on lamin.

Colocalization of the palmitoylation-deficient mutants of claudin-14 with the lysosomal membrane protein LAMP-2. Colocalization of the palmitoylation-deficient.

Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes.

Presentation transcript:

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins A–H Endogenous MOSPD2 (green) staining in HeLa cells expressing Flag‐STARD3 (A), Flag‐STARD3 FA/YA (B), GFP‐ORP1L (C), GFP‐ORP1L FA/YA (D), Flag‐STARD11 (E), Flag‐STARD11 D324A (F), HA‐PTPIP51 (G), or HA‐PTPIP51 ΔFFAT (H) (magenta). Endogenous MOSPD2 was recruited around endosomes, Golgi, and mitochondria by STARD3 and ORP1L, STARD11 and PTPIP51, respectively. FFAT‐deficient STARD3, ORP1L, STARD11, and PTPIP51 mutants did not recruit endogenous MOSPD2. Note that in agreement with immunoprecipitation assays (Fig 5F), PTPIP51 ΔFFAT retained a partial ability to recruit MOSPD2. The subpanels on the right are higher magnification (3.5×) images of the area outlined in white. The Overlay panel shows merged green and magenta images. The Coloc panel displays a co‐localization mask on which pixels where the green and the magenta channels co‐localize are shown in white. Right: Linescan analyses with fluorescence intensities of the green and magenta channels along the white arrow shown on the subpanel Overlay. Black rectangles indicate the positions of late endosomes (E), Golgi stacks (G), and mitochondria (M). Scale bars: 10 μm. Thomas Di Mattia et al. EMBO Rep. 2018;19:e45453 © as stated in the article, figure or figure legend