Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis Elia Mattarucchi, Orietta.

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Molecular Monitoring of Residual Disease in Chronic Myeloid Leukemia by Genomic DNA Compared with Conventional mRNA Analysis Elia Mattarucchi, Orietta Spinelli, Alessandro Rambaldi, Francesco Pasquali, Francesco Lo Curto, Leonardo Campiotti, Giovanni Porta The Journal of Molecular Diagnostics Volume 11, Issue 5, Pages 482-487 (September 2009) DOI: 10.2353/jmoldx.2009.080150 Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Molecular follow-up of patient 8. A: Comparison of DNA and mRNA results during the 42 months of IM therapy. UND, undetectable levels. B: The amplification of 300 ng of peripheral blood DNA extracted after 42 months of therapy still reveals disease eradication. C: The same amount of DNA from a healthy participant was positive for the control (BCR) but not for the BCR-ABL sequence. D: Amplification of 300 ng of DNA from patient 8, subsequently diluted 1:10 into the DNA from a healthy participant, demonstrates a sensitivity greater than the minimum value of 10−4 dilutions. The Journal of Molecular Diagnostics 2009 11, 482-487DOI: (10.2353/jmoldx.2009.080150) Copyright © 2009 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions