A panel of PCR-inhibitory reference materials for quality evaluation of multiplex STR analysis kits Malin Sanga, Lina Boiso, Harwati Lindsten, Peter Rådström, Ricky Ansell, Johannes Hedman Forensic Science International: Genetics Supplement Series Volume 5, Pages e317-e319 (December 2015) DOI: 10.1016/j.fsigss.2015.09.126 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions
Fig. 1 Different effects of inhibitory substances. The blue panel of ESX Fast is shown for four different scenarios. (A) positive control. The amel/D3S1358 and D18S51/D3S1358 ratios were 0.8 and 1.0. (B) Addition of soil (5μL) giving partial inhibition with a specific effect on longer markers. The amel/D3S1358 and D18S51/D3S1358 ratios were 0.5 and 0. (C) Addition of humic acid (2μg) inhibiting amelogenin. The amel/D3S1358 and D18S51/D3S1358 ratios were 0.2 and 0.3. (D) Addition of moist snuff (5μL) giving complete inhibition. Forensic Science International: Genetics Supplement Series 2015 5, e317-e319DOI: (10.1016/j.fsigss.2015.09.126) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions