Fig. 4. Ex vivo and in vivo function of the MeTro sealant using rat incision model of arteries. Ex vivo and in vivo function of the MeTro sealant using.

Slides:

Advertisements

Similar presentations

Fig. 1. TP is highly expressed in myeloma.

Advertisements

Fig. 3. Ultrasound-mediated BMP-6 gene delivery to mini-pig tibial bone fractures. Ultrasound-mediated BMP-6 gene delivery to mini-pig tibial bone fractures.

Fig. 5. Circulating PPi concentration does not correlate with severity of calcification phenotype in mice. Circulating PPi concentration does not correlate.

In vivo function of MeTro sealants using rat incision model of lungs

Fig. 6. Ex vivo and in vivo test to evaluate the sealing capability of the MeTro sealant using a porcine lung incision model. Ex vivo and in vivo test.

Fig. 4. Biomechanical properties of treated tibiae.

Fig. 4. aPD-1 mAb transfer to macrophages is mediated by FcγRs.

Fig. 3. Frequencies of amino acids at critical PGT121 and contact sites in the SHIV-SF162P3 challenge stock. Frequencies of amino acids at critical.

Fig. 3. 3RMR response to graded ischemia ex vivo.

Fig. 3. A circadian rhythm in fibroblast wound-healing response.

MRSA virulence proteins cause LMC death and diminished CLV function

Fig. 4 Infection-induced CLV dysfunction is associated with decreased LMC coverage. Infection-induced CLV dysfunction is associated with decreased LMC.

Fig. 2. In vitro sealing properties of the MeTro sealant.

Fig. 2. GPC3 expression in normal and tumor tissues.

Fig. 5. Antitumor efficacy of ERY974 in immunocompetent human CD3 transgenic mice. Antitumor efficacy of ERY974 in immunocompetent human CD3 transgenic.

Fig. 5. Pharmacological JAK2 inhibition in vivo abrogates tumor-initiating potential after chemotherapy. Pharmacological JAK2 inhibition in vivo abrogates.

Fig. 3. Wisper controls CF behavior and survival.

Fig. 5. Preliminary performance validation of the hydrogel in vivo in a rabbit model of scleral trauma. Preliminary performance validation of the hydrogel.

Fig. 4. Antitumor efficacy of ERY974 against various cancer types.

Fig. 1. IS mediates a hyperthrombotic uremic phenotype in an AHR-dependent manner across CKD stages. IS mediates a hyperthrombotic uremic phenotype in.

Fig. 3 Biological function of TG2 and the interaction with MT-2.

Fig. 1. CD31 is present throughout the human renal vasculature.

Fig. 7 Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors. Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors.

Fig. 5. In vivo characterization of adipogenesis by CT.

Fig. 5 Acute loss of mitochondrial content after impact is prevented by inhibition of electron transport or critical redox events. Acute loss of mitochondrial.

Fig. 6. A circadian rhythm in keratinocyte wound healing and a diurnal variation in human burn healing outcome. A circadian rhythm in keratinocyte wound.

Fig. 2. Vascular inflammation blocks perivascular adipocyte differentiation through paracrine signals. Vascular inflammation blocks perivascular adipocyte.

Fig. 2. Rheological characterization and hydrogel differentiation.

Fig. 3 Fbln4E57K/E57K mice develop large artery stiffness and systolic hypertension. Fbln4E57K/E57K mice develop large artery stiffness and systolic hypertension.

Fig. 6. Apoptotic MSCs exert in vivo immunosuppression in a TH2-type inflammation model in the absence of cytotoxic cells. Apoptotic MSCs exert in vivo.

Fig. 4. Expression of HGF in liver ECs cooperates with NOX4 inhibition to enhance engraftment of regenerative hepatocytes. Expression of HGF in liver ECs.

Fig. 8 SOD2 overexpression in normal tissues does not compromise the cytotoxic efficacy of RT. SOD2 overexpression in normal tissues does not compromise.

Fig. 4. Actin polymerization rhythms are required for circadian regulation of adhesion and wound-healing efficacy by fibroblasts. Actin polymerization.

Persistence of CAR4 cells is reduced after sustained TCR engagement

Fig. 4. Restriction of TCR antigen to hematopoietic tissues does not prevent CAR8 exhaustion and failure of leukemia clearance. Restriction of TCR antigen.

Fig. 3 hESC-RPE cell sheet transplantation and validation of the surgical method. hESC-RPE cell sheet transplantation and validation of the surgical method.

Fig. 7. KIF11 informs patient prognosis, and targeting improves survival in a preclinical model. KIF11 informs patient prognosis, and targeting improves.

Fig. 1. mGlu7 expression is reduced in RTT autopsy samples.

Fig. 3. Recovery of AVP-deficient rats from anemia induced by sublethal irradiation. Recovery of AVP-deficient rats from anemia induced by sublethal irradiation.

Fig. 2. Pathways differentially regulated in patients with early Lyme disease and STARI. Pathways differentially regulated in patients with early Lyme.

Fig. 2. Adenosine-induced SAN dysfunction recorded by optical mapping and SAN electrograms in ex vivo human hearts. Adenosine-induced SAN dysfunction recorded.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 7 Improvement of clinical score and axon pathology by nasal IL-4 treatment during chronic EAE. Improvement of clinical score and axon pathology by.

Fig. 6. Rolling neutrophils extract membranes from fragile remnant PS+ platelets. Rolling neutrophils extract membranes from fragile remnant PS+ platelets.

Fig. 6. Heterogeneous A1R and GIRK1/4 protein expression in human SAN and atria revealed by ex vivo molecular mapping. Heterogeneous A1R and GIRK1/4 protein.

Fig. 1. β-APP overexpression or exposure to inflammatory mediators induces sIBM-like pathology in cultured rat myocytes that is abrogated by arimoclomol.

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

PAAND is driven by local inflammasome activation and IL-1β production

Fig. 4. The effect of single-dose rozanolixizumab on the concentration of IgG subtypes in healthy subjects. The effect of single-dose rozanolixizumab on.

Fig. 6 Anticancer effects in PyMT-MMTV syngeneic and MDA-MB-231 xenograft-bearing mice. Anticancer effects in PyMT-MMTV syngeneic and MDA-MB-231 xenograft-bearing.

Evaluation of clinical responses after infusion of CART19 cells

Fig F-FGln shows uptake in human gliomas undergoing progression.

Fig. 3 CSF1 is expressed in human melanoma.

Fig. 1 Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Differentiation.

Fig. 7 Analysis of the bacterial nidus within tissue abscesses by MALDI IMS demonstrates a paucity of calprotectin signal. Analysis of the bacterial nidus.

Fig. 4. Local application of FR provides prolonged protection against Gq-dependent airway constriction in normal and OVA-sensitized mice in vivo. Local.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Fig. 7. Scale-up of AAV vector–mediated liver gene transfer of secretable GAA to nonhuman primates. Scale-up of AAV vector–mediated liver gene transfer.

Fig. 6 Combination therapy with LVSOD2 and LVshCTGF preserves flap volume and reduces fibrosis after RT. Combination therapy with LVSOD2 and LVshCTGF preserves.

Biocompatibility evaluation in vivo with a mouse subcutaneous implant

Fig. 1. MSCs undergo in vivo apoptosis after infusion without affecting immunosuppression. MSCs undergo in vivo apoptosis after infusion without affecting.

Fig. 1. Iontophoretic devices used for the delivery of cytotoxic agents to solid tumors. Iontophoretic devices used for the delivery of cytotoxic agents.

Fig. 6. Ex vivo and in vivo test to evaluate the sealing capability of the MeTro sealant using a porcine lung incision model. Ex vivo and in vivo test.

Fig. 1. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy.

Fig. 3. In vivo tumor responses are mechanism of action–specific and concentration-dependent. In vivo tumor responses are mechanism of action–specific.

Fig. 6 Pig 5 tissue development.

Fig. 2. In vitro sealing properties of the MeTro sealant.

In vivo function of MeTro sealants using rat incision model of lungs

Ex vivo application and adhesion properties of GelCORE adhesives

Fig. 2. Ex vivo and in vivo investigation of the mechanism of drug delivery enhancement with ultrasound. Ex vivo and in vivo investigation of the mechanism.

Presentation transcript:

Fig. 4. Ex vivo and in vivo function of the MeTro sealant using rat incision model of arteries. Ex vivo and in vivo function of the MeTro sealant using rat incision model of arteries. (A) Ex vivo wound closure test using explanted rat aorta as a biological substrate for tissue adhesion. (i) An explanted aorta from a rat (about 4 cm in length). (ii) A patch generated using the MeTro hydrogel and wrapped the artery tube segments. The connecting anastomosis points were further glued with MeTro. Arrows present the rat artery and MeTro sealant. (iii) Adhesive strength of the MeTro sealant applied on one side (n = 4) and both sides (n = 3) of the artery in comparison with Evicel applied on one side (n = 4) and both sides (n = 4). The MeTro patch (n = 2) further improved the adhesive strength. (B) In vivo tests on rat arteries sealed by MeTro (n = 3). Operative sites (i) before and (ii) after sealing. White arrow presents the MeTro sealant on the artery. (iii) Image of a sealed artery pressurized with air, demonstrating that MeTro could adhere to the outer arterial surface and seal the incision, but the artery burst in another area. Circles present the MeTro seals. Arrow indicates bubbles from the burst point on the artery instead of the MeTro sealing site. (iv) Burst pressure values of artery sealed by MeTro with 20% concentration and high methacryloyl substitution after 4 days compared to a healthy artery as a control. Representative SEM (C) and H&E-stained (D) images from the interface between the explanted rat artery and the MeTro sealant. Tight interfaces between the MeTro hydrogel and the tissues indicate strong bonding and interlocking at the interfaces. Data are means ± SD. P values were determined by one-way ANOVA followed by Tukey’s multiple comparisons test for (A) and (B) (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Nasim Annabi et al., Sci Transl Med 2017;9:eaai7466 Published by AAAS