HIV-specific effector cytotoxic T lymphocytes and HIV-producing cells colocalize in white pulps and germinal centers from infected patients by Anne Hosmalin, Assia Samri, Marie-Jeanne Dumaurier, Yasmine Dudoit, Eric Oksenhendler, Marina Karmochkine, Brigitte Autran, Simon Wain-Hobson, and Rémi Cheynier Blood Volume 97(9):2695-2701 May 1, 2001 ©2001 by American Society of Hematology

Detection of HIV-specific CTL effectors ex vivo from individual white pulps from the spleen of patient B.Target cells were autologous B-lymphoblastoid cells infected with either a mixture of recombinant vaccinia viruses expressing gag, pol, or env (●) or wi... Detection of HIV-specific CTL effectors ex vivo from individual white pulps from the spleen of patient B.Target cells were autologous B-lymphoblastoid cells infected with either a mixture of recombinant vaccinia viruses expressing gag, pol, or env (●) or wild-type vaccinia (○). Results from 11 representative white pulps of the 24 tested and from bulk SMCs are shown. Anne Hosmalin et al. Blood 2001;97:2695-2701 ©2001 by American Society of Hematology

Chromium release assays from the lymphoid organs of patients C, R, and K.Results from the bulk SMCs or TMCs and from one white pulp (WP) or germinal center (GC) of the 24 tested for each patient are shown. Chromium release assays from the lymphoid organs of patients C, R, and K.Results from the bulk SMCs or TMCs and from one white pulp (WP) or germinal center (GC) of the 24 tested for each patient are shown. Partially compatible B-lymphoblastoid cell lines were infected with wild-type vaccinia (wt; ○) or recombinant vaccinia viruses expressing gag, pol, and env (●) and used as targets. The HLA molecules shared between patient and target cells are shown in Table 1. Anne Hosmalin et al. Blood 2001;97:2695-2701 ©2001 by American Society of Hematology