Volume 7, Issue 2, Pages (July 2000)

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Volume 7, Issue 2, Pages 131-135 (July 2000) Enhancement of nitric oxide generation by low frequency electromagnetic field  Toshikazu Yoshikawa, Mari Tanigawa, Toru Tanigawa, Akito Imai, Hitoshi Hongo, Motoharu Kondo  Pathophysiology  Volume 7, Issue 2, Pages 131-135 (July 2000) DOI: 10.1016/S0928-4680(00)00040-7

Fig. 1 Experimental setup for exposure to magnetic field. A cage was placed vertical to the terrestorial magnetism, between Helmholtz electromagnets KMR 40. The EMF inside the cage was set at 0.1 mT. The control mice were placed in a cage with identical conditions but without artificial EMF. Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 2 Protocol of experiments. Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 3 EPR signal of NO adduct in mice liver. Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 4 EPR spectra of NO adduct in mice liver. The upper spectrum represents group C or D: LPS (+), the center spectrum shows group A: LPS (−) EMF (−) and the lower spectrum is a typical signal from group B: LPS (−) EMF (+). Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 5 The effects of EMF on NO generation induced by LPS (2 mg /kg). Signal intensity of mice liver of group D, C (LPS plus, with and without EMF, respectively) was significantly stronger than that of group A (control). Signal intensity of mice liver of group D (LPS plus, with EMF) was significantly higher than that of group C (LPS plus, without EMF). Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 6 The effects of EMF on NO generation induced by various doses of LPS. EMF significantly increased NO generation in mice treated with LPS at 2 and 10 mg/kg but not at 1 and 20 mg/kg. Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)

Fig. 7 Plasma nitrite level in mice treated with LPS at 2 mg/kg, measured as NO adducts (NO–MGD–Fe2+). EMF did not affect on plasma nitrite level in mice treated with LPS. Pathophysiology 2000 7, 131-135DOI: (10.1016/S0928-4680(00)00040-7)