Repression of contexual fear memory induced by isoflurane is accompanied by reduction in histone acetylation and rescued by sodium butyrate T. Zhong,

Slides:

Advertisements

Similar presentations

* Figure A in S1 File Body weight (g) n=8/group

Advertisements

The chemokine CCL2 activates p38 mitogen-activated protein kinase pathway in cultured rat hippocampal cells Jungsook Cho, Donna L. Gruol Journal of.

Seizure - European Journal of Epilepsy

Epileptiform discharge upregulates p-ERK1/2, growth-associated protein 43 and synaptophysin in cultured rat hippocampal neurons Zu-Cai Xu, Yang-Mei Chen,

Biphasic Alteration of the Inhibitory Synapse Scaffold Protein Gephyrin in Early and Late Stages of an Alzheimer Disease Model Eva Kiss, Karin Gorgas,

Electroacupuncture inhibits excessive interferon-γ evoked up-regulation of P2X4 receptor in spinal microglia in a CCI rat model for neuropathic pain

Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation K. Watanabe,

Small temperature variations alter edaravone-induced neuroprotection of cortical cultures exposed to prolonged hypoxic episodes S. Shibuta, S. Varathan,

Nerve conduction block in diabetic rats using high-intensity focused ultrasound for analgesic applications Y-F. Lee, C-C. Lin, J-S. Cheng, G-S. Chen

H. Wang, Y. Dong, J. Zhang, Z. Xu, G. Wang, C. A. Swain, Y. Zhang, Z

Xenon: no stranger to anaesthesia

Human factors in anaesthesia: lessons from aviation

Pain in cancer survivors; filling in the gaps

Presynaptic inhibition of the release of multiple major central nervous system neurotransmitter types by the inhaled anaesthetic isoflurane R.I. Westphalen,

Brain regional vulnerability to anaesthesia-induced neuroapoptosis shifts with age at exposure and extends into adulthood for some regions M. Deng, R.D.

X. Sun, J. Ellis, P.J. Corso, P.C. Hill, F. Chen, J. Lindsay

X. Luo, H. Zhao, L. Hennah, J. Ning, J. Liu, H. Tu, D. Ma

Type of anaesthesia and patient quality of recovery: a randomized trial comparing propofol–remifentanil total i.v. anaesthesia with desflurane anaesthesia

Parkinson's disease and anaesthesia

Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor- kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang,

Gabapentin prophylaxis for postoperative nausea and vomiting in abdominal surgeries: a quantitative analysis of evidence from randomized controlled clinical.

L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong

Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish Chuan Yan, Xiaojing Huo, Shu Wang,

Sevoflurane promotes the expansion of glioma stem cells through activation of hypoxia- inducible factors in vitro Q.Y. Shi, S.J. Zhang, L. Liu, Q.S. Chen,

G. Xu, H. Lu, Y. Dong, D. Shapoval, S. G. Soriano, X. Liu, Y. Zhang, Z

T.E. Howes, C.A. Lobo, F.E. Kelly, T.M. Cook

Volume 83, Issue 1, Pages (July 2014)

Isoflurane enhances the malignant potential of glioblastoma stem cells by promoting their viability, mobility in vitro and migratory capacity in vivo

L. Pain, A. Launoy, N. Fouquet, P. Oberling

Triaging the right patient to the right place in the shortest time

A different use of visual analytic techniques in anaesthetics

Retrospective analysis of the incidence of epidural haematoma in patients with epidural catheters and abnormal coagulation parameters P. Gulur, B. Tsui,

Pretreatment with intrathecal or intravenous morphine attenuates hepatic ischaemia– reperfusion injury in normal and cirrhotic rat liver Y. Wang, G.T.C.

Annexin 1 exerts anti-nociceptive effects after peripheral inflammatory pain through formyl-peptide-receptor-like 1 in rat dorsal root ganglion L. Pei,

S. Hagihira British Journal of Anaesthesia

Single sevoflurane exposure decreases neuronal nitric oxide synthase levels in the hippocampus of developing rats X. Feng, J.J. Liu, X. Zhou, F.H. Song,

Volume 20, Issue 15, Pages (August 2010)

Inflating LMA with normal saline: 14 yr after

A Richness that Cures Neuron

Correlating in vivo anaesthetic effects with ex vivo receptor density data supports a GABAergic mechanism of action for propofol, but not for isoflurane †‡

Late preconditioning with isoflurane in cultured rat cortical neurones

Loss of surface N-methyl-d-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo A. LacKamp, G.-C.

Neuroprotective and neurotoxic properties of the ‘inert’ gas, xenon

Volume 20, Issue 2, Pages (July 2017)

Sevoflurane post-conditioning increases nuclear factor erythroid 2-related factor and haemoxygenase-1 expression via protein kinase C pathway in a rat.

Inhibiting NADPH oxidase protects against long-term memory impairment induced by neonatal sevoflurane exposure in mice Z. Sun, M. Satomoto, Y.U. Adachi,

Isotonic crystalloid solutions: a structured review of the literature

Systemic oxygen extraction during exercise at high altitude

Argon reduces neurohistopathological damage and preserves functional recovery after cardiac arrest in rats A. Brücken, A. Cizen, C. Fera, A. Meinhardt,

Xenon decreases cell migration and secretion of a pro-angiogenesis factor in breast adenocarcinoma cells: comparison with sevoflurane S.A. Ash, G.I.

Inflammation-induced up-regulation of ionotropic glutamate receptor expression in human skin P.-H. Tan, L.-C. Yang, P.-T. Chiang, J.S.-C. Jang, H.-C.

L1–2 roots block with psoas compartment block?

Observational study of the success rates of intubation and failed intubation airway rescue techniques in 7256 attempted intubations of trauma patients.

Effects of different remifentanil target concentrations on MAC BAR of sevoflurane in gynaecological patients with CO2 pneumoperitoneum stimulus Z.-Y.

The third ultrasound dimension in anaesthesia and intensive care

Association of sustained cardiovascular recovery with epinephrine in the delayed lipid- based resuscitation from cardiac arrest induced by bupivacaine.

Alleviation of neuropathic pain by intrathecal injection of antisense oligonucleotides to p65 subunit of NF-κB T. Sun, W.-G. Song, Z.-J. Fu, Z.-H. Liu,

Volume 17, Issue 3, Pages (October 2016)

Chronic widespread pain, including fibromyalgia: a pathway for care developed by the British Pain Society J. Lee, B. Ellis, C. Price, A.P. Baranowski

Influence of ambient light on cerebral oximeters

Observational study of current use of selective decontamination of the digestive tract in UK Critical Care units R.R. Canter, S.E. Harvey, D.A. Harrison,

NAP5: the tip of the iceberg, or all we need to know?

Cut tracheal tube and GlideRite® Rigid Stylet

Suspected opioid-induced hyperalgesia in an infant

T. W. Jones, D. Houghton, S. Cassidy, G. A. MacGowan, M. I. Trenell, D

P.J. Moss, W. Huang, J. Dawes, K. Okuse, S.B. McMahon, A.S.C. Rice

Regional anaesthesia to improve pain outcomes in paediatric surgical patients: a qualitative systematic review of randomized controlled trials S. Suresh,

Controlled hypotension for middle ear surgery: a comparison between remifentanil and magnesium sulphate† J.-H. Ryu, I.-S. Sohn, S.-H. Do British Journal.

Apoptotic neurodegeneration and spatial memory are not affected by sedative and anaesthetics doses of ketamine/medetomidine combinations in adult mice

Irreplaceability of Neuronal Ensembles after Memory Allocation

Presentation transcript:

Repression of contexual fear memory induced by isoflurane is accompanied by reduction in histone acetylation and rescued by sodium butyrate T. Zhong, Q.J. Qing, Y. Yang, W.Y. Zou, Z. Ye, J.Q. Yan, Q.L. Guo British Journal of Anaesthesia Volume 113, Issue 4, Pages 634-643 (October 2014) DOI: 10.1093/bja/aeu184 Copyright © 2014 The Author(s) Terms and Conditions

Fig 1 (a) Timeline of experiment. (b) Freezing time of each group (n=14) during contexual fear conditioning (CFC) training and CFC testing the next day,** P<0.01 vs isoflurane (ISO)+vehicle group. Mean (sd) shown. (c) Freezing time of each group (n=12) during mock training and mock test in next day, were similar. Mean (sd) shown. British Journal of Anaesthesia 2014 113, 634-643DOI: (10.1093/bja/aeu184) Copyright © 2014 The Author(s) Terms and Conditions

Fig 2 Comparison of histone acetylation and c-Fos expression by immunoblotting between the air-exposed group and isoflurane-exposed group when the mice were in home cage (HC, n=6), at 1 h after mock training (MT, n=6) and 1 h after CFC training (n=6). Acetylation levels were measured by the ratio of H3 acetylation/total H3 or ratio of H4 acetylation/total H4, c-Fos expression was measured by c-Fos/β-actin ratio. *P<0.05, **P<0.01 vs HC+air group; ##P<0.01 vs MT+air group; &&P<0.01 vs CFC+air group. Results were normalized against the HC+air group, mean (sd) shown. British Journal of Anaesthesia 2014 113, 634-643DOI: (10.1093/bja/aeu184) Copyright © 2014 The Author(s) Terms and Conditions

Fig 3 Representative immunocytochemistry images of (a) H3K9 acetylation and (b) H3K14 acetylation in the CA1, CA3, and DG regions of each group (n=6) 1 h after CFC training. H3 acetylation in hippocampal neuronal nuclei is stained brown. (c) Relative OD value of H3K9 acetylation and (d) H3K14 acetylation in the CA1, CA3, and DG regions of each group 1 h after CFC training. The relative OD value of H3K14 acetylation in CA1 was reduced by exposure to isoflurane. Chronic systemic SB administration rescued isoflurane-induced reduction in H3K14 acetylation. *P<0.05 vs ISO+vehicle group. Results were normalized against the air+vehicle group, mean (sd) shown. (e) H3K9, H3K14 acetylation levels in the CA1 region via by immunoblotting 1 h after CFC training. H3K9, H3K14 acetylation, and total H3 immunoreactive densities are shown for each group (n=6). (f) Relative histone acetylation measured by the ratio of H3 acetylation/total H3, *P<0.05, **P<0.01 vs ISO+vehicle group. Results were normalized against the air+vehicle group, mean (sd) shown. British Journal of Anaesthesia 2014 113, 634-643DOI: (10.1093/bja/aeu184) Copyright © 2014 The Author(s) Terms and Conditions

Fig 4 Representative immunocytochemistry images of (a) H4K5 acetylation and (b) H4K12 acetylation in the CA1, CA3 and DG regions of each group 1 h after CFC training. H4 acetylation in hippocampal neuronal nuclei were stained brown by DAB. (c) Relative OD values of H4K5 acetylation and (d) H4K12 acetylation in CA1, CA3, and DG regions of each group 1 h after CFC training. Relative OD values of H4K5 acetylation in CA1 and DG regions, and H4K12 acetylation in the CA1 region were reduced by exposure to isoflurane. Chronic systemic SB administration rescued isoflurane-induced reduction in H4K5 and H4K12 acetylation. *P<0.05 vs ISO+vehicle group. Results were normalized against the air+vehicle group, mean (sd) shown. (e) H4K5, H4K12 acetylation in the CA1 region via by immunoblotting 1 h after CFC training. H4K5, H4K12 acetylation, and total H4 immunoreactive densities are shown for each group (n=6). (f) Relative histone acetylation measured by the ratio of H4 acetylation/total H4, *P<0.05, **P<0.01 vs ISO+vehicle group. Results were normalized against the air+vehicle group, mean (sd) shown. British Journal of Anaesthesia 2014 113, 634-643DOI: (10.1093/bja/aeu184) Copyright © 2014 The Author(s) Terms and Conditions

Fig 5 (a) Representative immunocytochemistry images of c-Fos in hippocampal region CA1, CA3, and DG of each group 1 h after CFC training. Hippocampal neuronal nuclei were stained purple with haematoxylin, nuclear c-Fos protein was stained brown. (b) c-Fos-positive cell counts. Mice exposed to isoflurane exhibited a reduction in c-Fos positive CA1 neurones 1 h after CFC training. Chronic systemic SB administration rescued isoflurane-induced reduction in c-Fos expression. No difference in c-Fos between the groups was observed in the CA3 and DG region. *P<0.05,**P<0.01 compared with the ISO+vehicle group. Mean (sd) shown. (c) c-Fos protein levels in CA1 determined via by immunoblotting 1 h after CFC training. c-Fos protein expression was measured by the ratio of c-Fos/β-actin, *P<0.05,**P<0.01 vs ISO+vehicle group (n=6). Results were normalized against air+vehicle group, mean (sd) shown. British Journal of Anaesthesia 2014 113, 634-643DOI: (10.1093/bja/aeu184) Copyright © 2014 The Author(s) Terms and Conditions