Effect of PRK and SRB on Mtb H37Rv survival

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Effect of PRK and SRB on Mtb H37Rv survival Effect of PRK and SRB on Mtb H37Rv survival ARepresentative image of Alamar blue assay employed for MIC determination.B, CMtb H37Rv cells were treated with varying concentration of inhibitors, and cell viability was determined using Alamar blue assay. MIC90 was calculated by plotting cell viability (%) against increasing concentration of inhibitors PRK and SRB.D, EHill's plot analysis of decline in Alamar blue fluorescence with increasing concentration of PRK and SRB, for IC50 determination.F, GCFU analysis of Mtb treated with PRK/SRB alone or a cocktail of Rif, Inh and Emb abbreviated as RHE and compared with a new combination of RH + PRK and RH + SRB, at two concentrations of PRK and SRB (0.1 and 1 μM), respectively. The x‐axis represents the time points post‐treatment with the inhibitors, and all the experiments were performed in triplicate and confirmed with biological duplicates at least. Statistical significance between experimental groups was determined by two‐tailed, unpaired Student's t‐test (****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.1).Data information: (D–G). Mean and standard error (SE) determined from three technical replicates (n = 3) and at least two biological duplicates. Archita Mishra et al. EMBO Mol Med. 2018;emmm.201708038 © as stated in the article, figure or figure legend