Consensus numbering system for the rifampicin resistance-associated rpoB gene mutations in pathogenic mycobacteria E. Andre, L. Goeminne, A. Cabibbe,

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Consensus numbering system for the rifampicin resistance-associated rpoB gene mutations in pathogenic mycobacteria E. Andre, L. Goeminne, A. Cabibbe, P. Beckert, B. Kabamba Mukadi, V. Mathys, S. Gagneux, S. Niemann, J. Van Ingen, E. Cambau Clinical Microbiology and Infection Volume 23, Issue 3, Pages 167-172 (March 2017) DOI: 10.1016/j.cmi.2016.09.006 Copyright © 2016 The Author(s) Terms and Conditions

Fig. 1 (a) Alignment of the nucleotide sequences for the rpoB genes of Escherichia coli, Mycobacterium tuberculosis H37Rv (MTB), Mycobacteirum leprae TN (ML) and Mycobacterium kansasii ATCC 12478 (MK). Rifampicin-resistance-determining region (RRDR) is highlighted in yellow. Red boxes show rifampicin resistance mediating mutation outside the RRDR. (b) Alignment of 81-bp RRDR of E. coli, M. tuberculosis complex, M. leprae and M. kansasii. Bars above the nucleotide sequences indicate the position of the seven codons frequently associated with rifampicin resistance, using the annotation system specific to each species. Nucleotide and amino acid differences are highlighted. Graph created using Geneious, Biomatters, version 9.1). Clinical Microbiology and Infection 2017 23, 167-172DOI: (10.1016/j.cmi.2016.09.006) Copyright © 2016 The Author(s) Terms and Conditions

Fig. 2 (a) Alignment of Mycobacterium tuberculosis complex rifampicin-resistance-determining region (RRDR) sequence with the targets of Xpert MTB/RIF and the MTBDRplus V2.0 commercial assays. The red bars represent the location of the most common codons associated with rifampicin resistance conferring mutations. The orange bars indicate the regions covered by the five probes of the Xpert MTB/RIF assay. The green bars represent the regions covered by the eight wild-type (WT) and four mutation bands of the Genotype MTBDRplus V2.0 assay. (b) Alignment of Mycobacterium leprae RRDR sequence with the targets of the LepraeDR commercial assay. The blue bars represent the location of the codons associated with rifampicin resistance conferring mutations, the four WT bands and the two mutation bands. Graph created using Geneious, Biomatters, version 9.1. Clinical Microbiology and Infection 2017 23, 167-172DOI: (10.1016/j.cmi.2016.09.006) Copyright © 2016 The Author(s) Terms and Conditions