Genetics & The Work of Mendel

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Genetics & The Work of Mendel 2006-2007

Gregor Mendel Modern genetics began in the mid-1800s in an abbey garden, where a monk named Gregor Mendel documented inheritance in peas used experimental method used quantitative analysis collected data & counted them excellent example of scientific method He studied at the University of Vienna from 1851 to 1853 where he was influenced by a physicist who encouraged experimentation and the application of mathematics to science and a botanist who aroused Mendel’s interest in the causes of variation in plants. After the university, Mendel taught at the Brunn Modern School and lived in the local monastery. The monks at this monastery had a long tradition of interest in the breeding of plants, including peas. Around 1857, Mendel began breeding garden peas to study inheritance.

Looking closer at Mendel’s work true-breeding purple-flower peas true-breeding white-flower peas X P 100% F1 generation (hybrids) purple-flower peas In a typical breeding experiment, Mendel would cross-pollinate (hybridize) two contrasting, true-breeding pea varieties. The true-breeding parents are the P generation and their hybrid offspring are the F1 generation. Mendel would then allow the F1 hybrids to self-pollinate to produce an F2 generation. self-pollinate F2 generation 3:1 75% purple-flower peas 25% white-flower peas

What did Mendel’s findings mean? Traits come in alternative versions purple vs. white flower color alleles different alleles vary in the sequence of nucleotides at the specific locus of a gene some difference in sequence of A, T, C, G purple-flower allele & white-flower allele are two DNA variations at flower-color locus different versions of gene at same location on homologous chromosomes

What did Mendel’s findings mean? Some traits mask others purple & white flower colors are separate traits that do not blend purple x white ≠ light purple purple masked white dominant allele functional protein masks other alleles recessive allele allele makes a malfunctioning protein homologous chromosomes

Law of Unit Characters Deduced there were units responsible for these traits. Units came in pairs Since sexual reproduction- each parent contributed one unit.

Law of Dominance Within any characteristic one allele appears more often than the other. Appears as one character is stronger than the other. Dominant allele selected to appear more often, recessive appears less.

Mendel’s 1st law of heredity PP P Law of segregation during meiosis, alleles segregate homologous chromosomes separate each allele for a trait is packaged into a separate gamete pp p Pp P p

Mendel’s 2nd law of heredity Law of independent assortment different loci (genes) separate into gametes independently non-homologous chromosomes align independently classes of gametes produced in equal amounts YR = Yr = yR = yr only true for genes on separate chromosomes or on same chromosome but so far apart that crossing over happens frequently yellow green round wrinkled YyRr Yr Yr yR yR YR YR yr yr 1 : 1 : 1 : 1

Punnett squares Pp x Pp F1 P p PP Pp P p PP Pp Pp Pp pp pp generation (hybrids) % genotype % phenotype P p male / sperm PP 25% 75% Pp 50% P p female / eggs PP Pp Pp Pp pp 25% 25% pp 1:2:1 3:1

Dihybrid cross YyRr x YyRr YR Yr yR yr YR Yr yR yr YYRR YYRr YyRR YyRr 9/16 yellow round YR Yr yR yr YR Yr yR yr 3/16 green round YYRR YYRr YyRR YyRr YYRr YYrr YyRr Yyrr 3/16 yellow wrinkled YyRR YyRr yyRR yyRr 1/16 green wrinkled YyRr Yyrr yyRr yyrr