Biosafety training BSL2/BSL3 TB

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Presentation transcript:

Biosafety training BSL2/BSL3 TB 10/11/2011

Policies needed for Biosafety in the Laboratory TB Lines of Defense in the Laboratory 2

Biosafety depends on Good Laboratory Practices NOTHING can totally eliminate the safety risk associated with the TB laboratory Good laboratory practices with emphasis on biosafety significantly reduces the risk of laboratory-acquired infection Specialized equipment aids good laboratory practice but does NOT replace it 3

Types of cabinets: General Product Protection Personal protection Product and personal protection

Types of cabinets: Biological safety cabinets Class II A HEPA-Filter Pre-filter Class II A (Double exhaust) Class II B

Types of cabinets: Biological safety cabinets Flow pattern 30% Downflow Exhaust Inflow Recirculation Pressure plenum 70%

Working safely in your BSC Escoglobal video http://www.youtube.com/watch?v=ZnUW1N-JJz8&feature=related Air flow patterns in a BSC GenomicaUASLP http://www.youtube.com/watch?v=Wg61LdngWlQ&feature=related

The primary risks to TB Laboratorians are : AEROSOLS Aerosols may include droplet nuclei containing 1-3 live bacilli that remain suspended in the air – a real danger for laboratory staff 8

Manipulations that produce Aerosols Pipetting Mixing Shaking Centrifugation Grinding Vortexing Pouring Use of squeeze bottles during processing Flaming loops or wet slides Opening tubes at non-ambient temperatures or pressures Pipet tip 9

How to Minimize Aerosols Always open and manipulate specimens and cultures in a certified biosafety cabinet (BSC) Use disposable loops or incinerators to sterilize loops Work over absorbent material soaked in disinfectant Use centrifuge safety cups and open them in the BSC Never express all the liquid in the pipette (leave the last bit inside the tip) Use caution when disrupting bubbles in tubes of liquid media 10

Specimen processing Can you identify any breaks in protocol?

Specimen processing Can you identify any breaks in protocol?

Specimen processing Can you identify any breaks in protocol?

Specimen processing Can you identify any breaks in protocol?

Specimen processing Can you identify any breaks in protocol?

Specimen processing Can you identify any breaks in protocol?

Specimen processing

Specimen processing

Specimen processing