Volume 23, Issue 3, Pages (September 2016)

Slides:

Advertisements

Similar presentations

Treating a collagen scaffold with a low concentration of nicotine promoted angiogenesis and wound healing Pham Hieu Liem, MD, MSc, Naoki Morimoto, MD,

Advertisements

Operant behavior to obtain palatable food modifies neuronal plasticity in the brain reward circuit Thomas Guegan, Laura Cutando, Eduard Ayuso, Emanuela.

DOI: /j.atherosclerosis

International Journal of Surgery

Contribution of nitric oxide synthase (NOS) in blood–brain barrier disruption during acute focal cerebral ischemia in normal rat Mohammad T. Mohammadi,

Inhibition of cell adhesion by anti–P-selectin aptamer: a new potential therapeutic agent for sickle cell disease by Diana R. Gutsaeva, James B. Parkerson,

Traditional extract of Pithecellobium dulce fruits protects mice against CCl4 induced renal oxidative impairments and necrotic cell death Pabitra Bikash.

Junctional adhesion molecule-A deficiency increases hepatic ischemia-reperfusion injury despite reduction of neutrophil transendothelial migration by Andrej.

A.O. Ige, E.O. Adewoye, N.C. Okwundu, O.E. Alade, P.C. Onuobia

The role of lymphatic vessels in the heart

International Journal of Surgery

Dextran sulfate modulates MAP kinase signaling and reduces endothelial injury in a rat aortic clamping model Yara Banz, MD, PhD, Thusitha Gajanayake,

Gale Tang, MD, David N. Charo, Rong Wang, PhD, Israel F

Ret mouse very large tumors (VLTs) display altered ratios of infiltrating memory to naive T cells: Roles in tumor expansion Mohammad W. Khan, Viktor.

Hypothermic protection in traumatic brain injury

Role of serotonin and nuclear factor-kappa B in the ameliorative effect of ginger on acetic acid-induced colitis Eman S.H. Abd Allah, Rania Makboul,

Intrathecal transplantation of bone marrow stromal cells attenuates blood-spinal cord barrier disruption induced by spinal cord ischemia-reperfusion injury.

Robert A. Brenes, MD, Caroline C

Ethyl pyruvate modulates delayed paralysis following thoracic aortic ischemia reperfusion in mice Bao-Ngoc Nguyen, MD, Hassan Albadawi, MD, Rahmi Oklu,

Do protons and X-rays induce cell-killing in human peripheral blood lymphocytes by different mechanisms? J. Miszczyk, K. Rawojć, A. Panek, A. Borkowska,

Volume 25, Issue 4, Pages (December 2018)

Ryan M. McEnaney, MD, Ankur Shukla, MD, Michael C

D. Neil Granger, Peter R. Kvietys Pathophysiology

Cellular and molecular mechanism regulating blood flow recovery in acute versus gradual femoral artery occlusion are distinct in the mouse Yagai Yang,

Volume 25, Issue 1, Pages (March 2018)

Increasing rates of head melanoma in Nordic countries

Pathophysiology of the lymphatic drainage of the central nervous system: Implications for pathogenesis and therapy of multiple sclerosis R.O. Weller,

Thiazolidinediones Regulate Adipose Lineage Dynamics

Galit Ankri-Eliahoo, PhD, Kevin Weitz, BS, Timothy C. Cox, PhD, Gale L

Neuroprotection following mild hypothermia after spinal cord ischemia in rats Takeshi Saito, PhD, Shino Saito, MD, Hiroshi Yamamoto, MD, PhD, Masanori.

Volume 18, Issue 19, Pages (October 2008)

S. Dangsriwan, P. Thanakiatkrai, W. Asawutmangkul, S. Phetpeng, T

Volume 24, Issue 12, Pages (June 2014)

The development of a forensic clock to determine time of death

Volume 23, Issue 4, Pages (December 2016)

FGL2 prothrombinase contributes to the early stage of coronary microvascular obstruction through a fibrin-dependent pathway Wen-Zhu Li, Yi Yang, Kun.

Vitamin D reduces high-fat diet induced weight gain and C-reactive protein, increases interleukin-10, and reduces CD86 and caspase-3 Asmaa M.S. Gomaa,

The Origin of Phragmoplast Asymmetry

Gilbert R. Kinsey, Liping Huang, Amy L. Vergis, Li Li, Mark D. Okusa

Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats Irshad Ali, PhD, Stephanie Gruenloh, BS, Ying Gao, MS,

A depleting antibody toward sca-1 mitigates a surge of CD34+/c-kit+ progenitors and reduces vascular restenosis in a murine vascular injury model Bryan.

Volume 75, Issue 7, Pages (April 2009)

Solutions to the discrepancies in the extent of liver damage following ischemia/reperfusion in standard mouse models Rowan F. van Golen, Megan J. Reiniers,

Increased Microvascular Density and Enhanced Leukocyte Rolling and Adhesion in the Skin of VEGF Transgenic Mice Michael Detmar, Lawrence F. Brown, Michael.

Volume 11, Issue 2, Pages (October 2004)

Subacute limb ischemia induces skeletal muscle injury in genetically susceptible mice independent of vascular density Joseph M. McClung, PhD, Timothy.

A novel in vivo murine model of cartilage regeneration

The role of short-term oxygen administration in the prevention of intimal hyperplasia Charu Lata, MBBS, Derrick Green, MD, Jing Wan, MD, Sabita Roy, PhD,

External Carotid Artery to Internal Carotid Artery Transposition with Patch Resection to Treat an Infected Carotid Endarterectomy Dacron Patch: A Novel.

Volume 85, Issue 4, Pages (April 2014)

Pathophysiological Characteristics of Melanoma In-Transit Metastasis in a Lymphedema Mouse Model Kohei Oashi, Hiroshi Furukawa, Hiroshi Nishihara, Michitaka.

Volume 75, Issue 5, Pages (March 2009)

Ischemic preconditioning ameliorates spinal cord ischemia-reperfusion injury by triggering autoregulation Cheng-Loong Liang, MD, Kang Lu, MD, PhD, Po-Chou.

Endoplasmic reticulum stress induced in motor neurons by transient spinal cord ischemia in rabbits Masahiro Sakurai, MD, PhD, Goro Takahashi, MD, PhD,

Ret mouse very large tumors (VLTs) display altered ratios of infiltrating memory to naive T cells: Roles in tumor expansion Mohammad W. Khan, Viktor.

An interstitial hypothesis for breast cancer related lymphoedema

Novel Anti-Inflammatory Properties of the Angiogenesis Inhibitor Vasostatin Rainer Huegel, Paula Velasco, Maria De La Luz Sierra, Enno Christophers, Jens.

Jun Asai, Hideya Takenaka, Norito Katoh, Saburo Kishimoto

Dnamatch2: An open source software to carry out large scale database searches of mixtures using qualitative and quantitative models Ø. Bleka, M. Bouzga,

Pathophysiology of iron overload-induced renal injury and dysfunction: Roles of renal oxidative stress and systemic inflammatory mediators A.O. Ige,

Colombian results of the interlaboratory quality control exercise 2015

Volume 25, Issue 1, Pages (March 2018)

B. Martínez, R. Pereira, K. Meza, L. Hernández, A. Amorim, J

David Olawale Adeyemi, Paul Olugbemiga Awoniran

John E. Pickard, Klaus Ley Biophysical Journal

A sphingosine-1-phosphate type 1 receptor agonist inhibits the early T-cell transient following renal ischemia–reperfusion injury L.-W. Lai, K.-C. Yong,

Remodeling of experimental arteriovenous fistula with increased matrix metalloproteinase expression in rats Chih-Yang Chan, MD, Yih-Sharng Chen, MD,

Beyond T2 and 3T: New MRI techniques for clinicians

Volume 38, Issue 1, Pages (January 2003)

Galit Ankri-Eliahoo, PhD, Kevin Weitz, BS, Timothy C. Cox, PhD, Gale L

Presentation transcript:

Volume 23, Issue 3, Pages 221-228 (September 2016) Dynamics of early stem cell recruitment in skin flaps subjected to ischemia reperfusion injury Ya Hui Tang, MD, PhD, Lindsey A. Pennington, MD, Jessica W. Scordino, MD, Jonathan Steven Alexander, PhD, Timothy Lian, MD Pathophysiology Volume 23, Issue 3, Pages 221-228 (September 2016) DOI: 10.1016/j.pathophys.2016.07.001 Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig 1 Percent necrosis in postischemic skin flap of control and experimental mice with BMSCs treatment. The percent necrosis decreased significantly in experimental mice comparison with control on day 3, 5 and 7 post I/R. Sham: n=5; Ischemia control: n=7; Ischemia+BMSC: n=6. ** p<0.005. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 2 Macroscopic appearance of control (Sham, left panel), post-ischemic (I/R, middle panel) and BMSC-treated post-ischemic (I/R+BMSC, right panel) skin flaps in mice at Day 3 post-surgery. Yellow dots: Necrosis areas. Scale bar: 5mm (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.). Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 3 Rolling velocity of BMSCs and leukocytes in skin flap venules of control and postischemic (I/R) mice. Control and I/R mice n=6, respectively. * p<0.05 and ** p<0.005 relative to leukocyte. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 4 A: Magnified image of 1×2cm flap mapped on the right abdomen area of a C57 Bl/6J mouse. B: An image of the inferior epigastric vessels (black arrow) pedicled island flap raised with a pedicle clamp in place. Black rectangle: distal area (6×3mm) from edge of incision toward pedicle; Green square: proximal area (3×3mm). C & D: Photomicrographs of a skin flap from a control mouse showing the trafficking of BMSC and leukocytes in situ 2h following injection i.v. of the CFSE labeled BMSC obtained by intravital fluorescent microscopy. C: a BMSC (white arrowhead) adhered to a wall of venule and the other BMSC was rolling within the vessel (direction of flow is indicated by the red arrowhead) of a raised skin flap. D: Many BMSC (white arrow) and leukocytes transmigrated into interstitial of distal area. Black arrow: leukocyte. Black arrowhead: adipocyte. Scale bar=100μm. E: Schematic of time points of I/R, BMSCs injection and microscopy examination. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 5 Flux fraction of BMSCs (rolling number of BMSCs divided by total rolling plus free flow numbers of BMSCs; A) and leukocytes (B) rolling were analysed in skin flap venules of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively. * p<0.05. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 6 BMSCs adhesion percentage per minute (number of BMSCs adhesion divided by total numbers of flux plus adhesion of BMSCs per minute; A) and leukocytes adhesion percentage per minute (B) were analysed in skin flap venules of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 7 Transmigrated BMSCs (A) and leukocytes (B) were observed in the interstitial of skin flap of control and postischemic (I/R) mice 2 and 4h post reperfusion. Control and I/R mice n=6, respectively. * p<0.05, ** p<0.005. # indicates leukocyte compared to BMSC correspondently p<0.05, ## p<0.005. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions

Fig. 8 Transmigrated BMSCs were observed in the proximal and distal part of skin flap of control and postischemic (I/R) mice 2 (A) and 4h (B) post reperfusion. Control and I/R mice n=6, respectively. * p<0.05, ** p<0.005. Pathophysiology 2016 23, 221-228DOI: (10.1016/j.pathophys.2016.07.001) Copyright © 2016 Elsevier B.V. Terms and Conditions