Holliday model Strand exchange and Heteroduplex DNA formation

Slides:

Advertisements

Similar presentations

Homologous and Site-Specific Replication Chapter 19.

Advertisements

Homologous Recombination at the Molecular Level

Most UV lesions are repaired by Nucleotide Excision Repair (NER) Stalled replication forks may be bypassed by alternative (bypass) DNA polymerases (REV1,

Molecular Biology Fourth Edition

Mechanism of Homologous Recombination

Molecular Genetics 2010 Welcome to the course!. Molecular Genetics 2008 Welcome to the course! Describes the use of Molecular Genetics to study a range.

Andrew J. Pierce TOX 780 Single and Double-strand Break Repair TOX 780 Andrew Pierce Microbiology, Immunology and Molecular Genetics Toxicology University.

Welcome Each of You to My Molecular Biology Class.

The role of the S. pombe MRN complex in the removal of covalently bound protein from the DNA Edgar Hartsuiker Kenichi Mizuno Tony Carr.

Chapter 6 Molecular Biology of DNA Replication and Recombination Jones and Bartlett Publishers © 2005.

Chapter 5 General Recombination.

CHAPTER 19 M ECHANISMS OF R ECOMBINATION. Recombination occurs at regions of homology between chromosomes through the breakage and reunion of DNA molecules.

Objectives of DNA recombination

Molecular Biology Fifth Edition

Models of Recombination. His thr arg Fogel and Hurst Meiotic gene conversion in yeast tetrads and the theory of recombination. Genetics.

Mechanisms of Homologous Recombination & Protein Rad51 Jia Liu Adam Jara Jan 20 th, 2011 MCB 720.

3.12 Gene Knockouts and Transgenics transgenics – Organisms created by introducing DNA prepared in test tubes into the germline. –The DNA may be inserted.

DNA REPLICATION 1) Semiconservative Replication According to this model, DNA Replication would create two molecules. Each of them would be a complex of.

Maintenance of genomes Copying the genome sequence (replication) Repairing damage to the genome sequence Rearranging genome sequences.

Homologous Recombination

DNA R ECOMBINATION M ECHANISMS Fahareen Binta Mosharraf MNS 1.

Mechanism of Homologous Recombination (HR)

Genetics: From Genes to Genomes

Office Hours (midterm #1)

Mus81 and Yen1 Promote Reciprocal Exchange during Mitotic Recombination to Maintain Genome Integrity in Budding Yeast Chu Kwen Ho, Gerard Mazón, Alicia.

Box Mutations in BRCA1/2 and Rad51 are associated with breast cancer

Concerted evolution Concerted evolution: the observation that paralogous genes appear "homogenized" (e.g., little difference in known non-functional region.

The Saccharomyces cerevisiae Msh2 Mismatch Repair Protein Localizes to Recombination Intermediates In Vivo Elizabeth Evans, Neal Sugawara, James E Haber,

Mutations + Recombination = Genetic Variation!!

DNA Replication Reaches the Breaking Point

Control of Crossing Over

Randy W. Hyppa, Gerald R. Smith Cell

Chapter 15 Homologous and Site-Specific Recombination

Mus81 and Yen1 Promote Reciprocal Exchange during Mitotic Recombination to Maintain Genome Integrity in Budding Yeast Chu Kwen Ho, Gerard Mazón, Alicia.

Volume 29, Issue 4, Pages (February 2008)

Recombination Mechanisms

Homologous Recombination

Volume 10, Issue 2, Pages (August 2002)

Pervasive and Essential Roles of the Top3-Rmi1 Decatenase Orchestrate Recombination and Facilitate Chromosome Segregation in Meiosis Shangming Tang,

Playing the End Game: DNA Double-Strand Break Repair Pathway Choice

Hsin-Yen Wu, Hsuan-Chung Ho, Sean M. Burgess Current Biology

Thorsten Allers, Michael Lichten Cell

Neal Sugawara, Xuan Wang, James E. Haber Molecular Cell

Volume 46, Issue 1, Pages (April 2012)

Hideo Tsubouchi, G.Shirleen Roeder Developmental Cell

Volume 130, Issue 2, Pages (July 2007)

Single Holliday Junctions Are Intermediates of Meiotic Recombination

The Fuss about Mus81 James E Haber, Wolf-Dietrich Heyer Cell

Intermediates of Yeast Meiotic Recombination Contain Heteroduplex DNA

Homologous Recombination

Early Decision Cell Volume 117, Issue 1, Pages 9-15 (April 2004)

Brh2 Promotes a Template-Switching Reaction Enabling Recombinational Bypass of Lesions during DNA Synthesis Nayef Mazloum, William K. Holloman Molecular.

Homologous recombination

The DNA Damage Response: Making It Safe to Play with Knives

Jill E. Falk, Andrew Chi-ho Chan, Eva Hoffmann, Andreas Hochwagen

Allyson M Holmes, James E Haber Cell

The Impressionistic Landscape of Meiotic Recombination

Rad52 Uffe H. Mortensen, Michael Lisby, Rodney Rothstein

Homology Requirements and Competition between Gene Conversion and Break- Induced Replication during Double-Strand Break Repair Anuja Mehta, Annette Beach,

Volume 29, Issue 2, Pages (February 2008)

Volume 11, Issue 6, Pages (June 2003)

Recombination: Holliday Junction Resolution and Crossover Formation

Volume 117, Issue 1, Pages (April 2004)

At Loose Ends: Resecting a Double-Strand Break

Volume 95, Issue 5, Pages (November 1998)

Volume 9, Issue 2, Pages (February 2002)

The DNA Damage Response: Making It Safe to Play with Knives

Gene Amplification: Yeast Takes a Turn

Volume 57, Issue 4, Pages (February 2015)

Sex and the Single (Double-Strand) Break

Presentation transcript:

Holliday model Strand exchange and Heteroduplex DNA formation HJ resolution Mismatch repair

Meselson-Radding 1975

Szostak Orr-Weaver Rothstein Stahl (1983) model

Synthesis Dependent Strand Annealing

Rad51-dependent ectopic (interchromosomal) gene conversion in haploid cells HO endonuclease-induced DSB Non-crossover Crossover

Crossovers and Noncrossovers occur with different kinetics wild type noncrossover crossover crossover noncrossover Crossovers and Noncrossovers occur with different kinetics Analogous results in yeast meiosis (Allers and Lichten 2001)

Modified SDSA SzOrRoST (1983)

Meiotic recombination Axial element Spo11 Mre11 Rad50 etc DSB Synaptonemal complex Timing of meiotic DSBs: 2 hr after replication. Suggests some structures are assembled during replication that are needed to create DSB

Kinetics of meiotic recombination initiated by a DSB Double HJ 2D gels 2D gels, as used to examine replication

Analysis of Joint Molecule intermediates by denaturing gel electrophoresis Is it this ? Or this? This