Characterization of pollen antigen–induced IL-31 production by PBMCs in patients with allergic rhinitis Mitsuhiro Okano, MD, Tazuko Fujiwara, BS, Takaya.

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Presentation transcript:

Characterization of pollen antigen–induced IL-31 production by PBMCs in patients with allergic rhinitis Mitsuhiro Okano, MD, Tazuko Fujiwara, BS, Takaya Higaki, MD, Seiichiro Makihara, MD, Takenori Haruna, MD, Yohei Noda, MD, Kengo Kanai, MD, Shin Kariya, MD, Hiroshi Yasueda, PhD, Kazunori Nishizaki, MD Journal of Allergy and Clinical Immunology Volume 127, Issue 1, Pages 277-279.e11 (January 2011) DOI: 10.1016/j.jaci.2010.09.029 Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Production of IL-31 by PBMCs after stimulation with pollen antigens. PBMCs from control subjects (Control), patients with JCCP without specific immunotherapy (Non-SIT), and patients with JCCP with specific immunotherapy (SIT) were stimulated with Cry j 1 (A), cedar crude antigen (B), and cypress crude antigen (C), and then the concentration of IL-31 in the supernatants was measured. The horizontal line indicates the median. P values were determined by using the Mann-Whitney U test. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Comparison of naso-ocular symptoms (A-C) and QOL (D-F) during the peak season of cypress pollen dispersion between patients with positive and negative production of IL-31 in response to Cry j 1 (Fig 2, A and D), cedar crude antigen (Fig 2, B and E), and cypress crude antigen (Fig 2, C and F). The horizontal line indicates the median. P values were determined by using the Mann-Whitney U test. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Relationship between amounts of pollen antigen–induced IL-31 and TH1/TH2 cytokines produced by PBMCs from patients with JCCP. Correlations between the amounts of IL-31 in response to Cry j 1 (A-C), cedar crude antigen (D-F), and cypress crude antigen (G-I) and the amounts of IL-5 (Fig E1, A, D, and G), IL-13 (Fig E1, B, E, and H), and IFN-γ (Fig E1, C, F, and I) in response to the respective antigens were determined by using the Spearman correlation coefficient by rank. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of the amounts of IL-5 (A, D, and G), IL-13 (B, E, and H), and IFN-γ (C, F, and I) in response to Cry j 1 (Fig E2, A-C), cedar crude antigen (Fig E2, D-F), and cypress crude antigen (Fig E2, G-I) between patients with positive and negative production of IL-31 in response to the respective antigens. The horizontal line indicates the median. P values were determined by using the Mann-Whitney U test. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Comparison of naso-ocular symptoms (A-C) and QOL (D-F) during the peak season of cedar pollen dispersion between patients with positive and negative production of IL-31 in response to Cry j 1 (Fig E3, A and D), cedar crude antigen (Fig E3, B and E), and cypress crude antigen (Fig E3, C and F). The horizontal line indicates the median. P values were determined by using the Mann-Whitney U test. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Relationship between naso-ocular symptoms (A-C) and QOL (D-F) during the peak season of cedar pollen dispersion and the amounts of IL-31 produced by PBMCs in response to Cry j 1 (Fig E4, A and D), cedar crude antigen (Fig E4, B and E), and cypress crude antigen (Fig E4, C and F). The ρ and P values were determined by using the Spearman correlation coefficient by rank. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Relationship between naso-ocular symptoms (A-C) and QOL (D-F) during the peak season of cypress pollen dispersion and the amounts of IL-31 produced by PBMCs in response to Cry j 1 (Fig E5, A and D), cedar crude antigen (Fig E5, B and E), and cypress crude antigen (Fig E5, C and F). The ρ and P values were determined by using the Spearman correlation coefficient by rank. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Relationship between QOL during the peak season of cypress pollen dispersion and the amounts of IL-5 (A-C) and IL-13 (D-F) produced by PBMCs in response to Cry j 1 (Fig E6, A and D), cedar crude antigen (Fig E6, B and E), and cypress crude antigen (Fig E6, C and F). The ρ and P values were determined by using the Spearman correlation coefficient by rank. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fluctuation of cedar and cypress pollen dispersion from February to April 2009. Open squares and filled circles represent the counts of cedar and cypress pollen, respectively. Naso-ocular symptoms and rhinitis-related QOL were monitored during the peak season of cedar pollen dispersion (March 4-18, solid line) and cypress pollen dispersion (April 8-22, dotted line) by using Japanese rhinoconjunctivitis QOL questionnaire number 1. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Production of TH1 and TH2 cytokines of PBMCs against stimulation with pollen antigens. PBMCs from healthy control subjects (Control), patients with JCCP without specific immunotherapy with standardized extract of Japanese cedar pollen (Non-SIT), and patients with JCCP with specific immunotherapy (SIT) were stimulated with Cry j 1 (A-C), cedar crude antigen (D-F), and cypress crude antigen (G-I) for 72 hours, and then the concentration of IL-5 (Fig E8, A, D, and G), IL-13 (Fig E8, B, E, and H), and IFN-γ (Fig E8, C, F, and I) in the supernatants was measured by means of ELISA. The rectangle includes the range from the 25th and 75th percentiles, the horizontal line indicates the median, and the vertical line indicates the range from the 10th to the 90th percentiles. P values were determined by using the Mann-Whitney U test. Ag, Antigen. Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Relationship between Cry j 1– and Cha o 1–induced IL-5 production by PBMCs from patients with JCCP (n = 46). Journal of Allergy and Clinical Immunology 2011 127, 277-279.e11DOI: (10.1016/j.jaci.2010.09.029) Copyright © 2010 American Academy of Allergy, Asthma & Immunology Terms and Conditions