ADVANCED TARGETING SYSTEMS for specific elimination of cell types 7
1. Get an antibody specific to your cell type Or, you can use a growth factor, peptide, or cytokine ANTIBODY 7
2. Use a potent toxin that is safe in the lab, but lethal inside a cell. SAPORIN 7
3. ATS puts them together to make a powerful targeting agent 7
Targeted Toxins can be used in vitro or in vivo 7
Targeted Toxins can be used in vitro or in vivo 7
The antibody seeks out its target receptor on the cell’s surface. 7
The antibody seeks out its target receptor on the cell’s surface. 7
Without its escort, SAPORIN cannot get inside a cell. 7
Without its escort, SAPORIN cannot get inside a cell. 7
Once attached to its target receptor, the conjugate is internalized. 7
Once attached to its target receptor, the conjugate is internalized. 7
Once attached to its target receptor, the conjugate is internalized. 7
Once attached to its target receptor, the conjugate is internalized. 7
have the target receptor will not be affected. Cells which do not have the target receptor will not be affected. 7
have the target receptor will not be affected. Cells which do not have the target receptor will not be affected. 7
have the target receptor will not be affected. Cells which do not have the target receptor will not be affected. 7
have the target receptor will not be affected. Cells which do not have the target receptor will not be affected. 7
have the target receptor will not be affected. Cells which do not have the target receptor will not be affected. 7
The SAPORIN conjugate enters the cell. 7
The SAPORIN conjugate enters the cell. 7
The SAPORIN conjugate enters the cell. 7
The SAPORIN conjugate enters the cell. 7
Once inside, SAPORIN will inactivate the ribosome. 7
Once inside, SAPORIN will inactivate the ribosome. 7
Once inside, SAPORIN will inactivate the ribosome. 7
The result is cell death. 7
The result is cell death. 7
The result is cell death. 7
The conjugate is carried inside the cell. coated vesicle 7
coated vesicle 7
coated vesicle 7
coated vesicle 7
coated vesicle 7
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7
Saporin escapes Ribosomes Golgi 7
Ribosomes Golgi 7
Ribosomes Golgi 7
SAPORIN inactivates the ribosome Ribosomes SAPORIN inactivates the ribosome Golgi 7
RIP Ribosomes SAPORIN inactivates the ribosome R I P Golgi 7
SAPORIN inactivates the ribosome R I P 7
SAPORIN inactivates the ribosome R I P R I P 7
R I P R I P 7
R I P R I P R I P 7
The result is Cell Death R I P R I P R I P 7
R I P R I P R I P R I P 7
R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P R I P R I P 7
R I P R I P R I P R I P R I P R I P R I P 7
ADVANCED TARGETING SYSTEMS for specific elimination of cell types 7
Saporin Saporin (pronounced sap – r – n) e e ⏝ Saporin (pronounced sap – r – n) e e Saporin is a ribosome-inactivating protein. Molecular weight – 30 kDa From the seeds of the plant, Saponaria officinalis Saporin is safe for laboratory use under normal safety conditions LD50 in mice – 4 mg/kg Saporin does not have a method of cell entry on its own, except through bulk-phase endocytosis
Advanced Targeting Systems also provides Custom Conjugations to use your Targeting Agent to deliver any Payload. Targeting Agent * Antibody Peptide Ligand Aptamer Growth Factor Cytokine Payload Toxin Drug Killing Agent Activating Agent Reversible Agent * Anything that is recognized on the cell surface and internalizes www.ATSbio.com