Volume 75, Issue 1, Pages (January 2009)

Slides:



Advertisements
Similar presentations
Volume 79, Issue 8, Pages (April 2011)
Advertisements

Glucose handling by the kidney
Volume 78, Issue 2, Pages (July 2010)
Ruisheng Liu, Oscar A. Carretero, Yilin Ren, Jeffrey L. Garvin 
The Case ∣ Acute renal failure and anemia
Michael Fritzenwanger, Ines Heid, Adolf Dörge  Kidney International 
IGF-1 vs insulin: Respective roles in modulating sodium transport via the PI-3 kinase/Sgk1 pathway in a cortical collecting duct cell line  E. Gonzalez-Rodriguez,
Volume 72, Issue 4, Pages (August 2007)
Volume 79, Issue 8, Pages (April 2011)
Making sense of the sensor: Mysteries of the macula densa
John M. Arthur, M.D.Ph.D., Shazia Shamim  Kidney International 
Inhibition of proximal convoluted tubule transport by dopamine
Volume 78, Issue 10, Pages (November 2010)
A trail of research on potassium
Renal and extrarenal regulation of potassium
Volume 84, Issue 2, Pages (August 2013)
Autosomal dominant distal renal tubular acidosis and the AE1 gene
Volume 71, Issue 5, Pages (March 2007)
Volume 73, Issue 2, Pages (January 2008)
Volume 55, Issue 6, Pages (June 1999)
Volume 57, Issue 6, Pages (June 2000)
Volume 56, Issue 4, Pages (October 1999)
Volume 66, Issue 2, Pages (August 2004)
Comorbidity and confounding in end-stage renal disease
Volume 57, Issue 6, Pages (June 2000)
Vasopressin in chronic kidney disease: an elephant in the room?
NH4+ secretion in inner medullary collecting duct in potassium deprivation: Role of colonic H+-K+-ATPase  Suguru Nakamura, Hassane Amlal, John H. Galla,
Volume 57, Issue 4, Pages (April 2000)
Volume 86, Issue 2, Pages (August 2014)
Regulation of renal tubular secretion of organic compounds
Volume 53, Issue 4, Pages (April 1998)
R. Willi Grunewald, Claudius H. Reisse, Gerhard A. Müller 
Timothy A. Sutton, Pierre C. Dagher  Kidney International 
Karl Kunzelmann, Rainer Schreiber, Anissa Boucherot 
Carol M. Herak-Kramberger, Dennis Brown, Ivan Sabolić 
Glucocorticoids enhance the expression of the basolateral Na+:HCO3- cotransporter in renal proximal tubules  Raza Ali, Hassane Amlal, Charles E. Burnham,
Volume 74, Issue 4, Pages (August 2008)
Colonic H+-K+-ATPase is induced and mediates increased HCO3− reabsorption in inner medullary collecting duct in potassium depletion  Suguru Nakamura,
Volume 82, Issue 1, Pages 3-4 (July 2012)
Signaling path of the action of AVP on distal K+ secretion
Two channels for one job
Volume 69, Issue 12, Pages (June 2006)
Hyperkalemia: An adaptive response in chronic renal insufficiency
Volume 79, Issue 4, Pages (February 2011)
Volume 74, Issue 8, Pages (October 2008)
Reemergence of the maxi K+ as a K+ secretory channel
Volume 84, Issue 2, Pages (August 2013)
Dr Antonio Di Stefano, Sylvie Jounier, Monika Wittner 
Nephrology Crossword: Glomerulonephritis
Volume 80, Issue 4, Pages (August 2011)
Insulin resistance and hypertension: new insights
Dr Brian J. Harvey, Maria Higgins  Kidney International 
Renal potassium channels: Function, regulation, and structure
Volume 82, Issue 9, Pages (November 2012)
The treatment of acute interstitial nephritis: More data at last
Neena Gupta, Sahar R. Tarif, Mouin Seikaly, Michel Baum, M.D. 
Volume 53, Issue 5, Pages (May 1998)
Ionic transport in macula densa cells
A new regulator of the vacuolar H+-ATPase in the kidney
Volume 86, Issue 1, Pages (July 2014)
Volume 72, Issue 4, Pages (August 2007)
Volume 77, Issue 1, Pages (January 2010)
Volume 76, Issue 8, Pages (October 2009)
Glucose handling by the kidney
Ammonia secretion by the collecting duct.
Volume 75, Issue 7, Pages (April 2009)
Volume 70, Issue 1, Pages (July 2006)
Volume 76, Issue 9, Pages (November 2009)
Aldosterone and potassium secretion by the cortical collecting duct
Volume 55, Issue 1, Pages (January 1999)
Presentation transcript:

Volume 75, Issue 1, Pages 25-30 (January 2009) Basolateral Na+/H+ exchange maintains potassium secretion during diminished sodium transport in the rabbit cortical collecting duct  Shigeaki Muto, Shuichi Tsuruoka, Yukio Miyata, Akio Fujimura, Eiji Kusano, WenHui Wang, Donald Seldin, Gerhard Giebisch  Kidney International  Volume 75, Issue 1, Pages 25-30 (January 2009) DOI: 10.1038/ki.2008.447 Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 1 Typical tracings showing effects of raising bath K+ from 2.5 to 8.5 mM in absence and presence of bath Ba2+ (2 mM) on VT and VB in the CCD. The luminal Na+ concentration was 0 mM. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 2 Bar graph summarizing effects of raising bath K+ from 2.5 to 8.5 mM on the basolateral membrane conductance (GB), apical membrane conductance (GA), and tight junction conductance (GTJ). *P<0.05 and **P<0.001 compared with 2.5 mM bath K+ (2.5K). The number of tubules examined is 18. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 3 Effects of raising bath K+ from 2.5 to 8.5 mM on net K+ secretion (JK) in CCDs perfused in vitro in the presence of lumen Na+ (146.8 mM) or absence of lumen Na+. Asterisk indicates the significant difference with 8.5 mM K+ in the bath (8.5K) in comparison to 2.5 mM K+ in the bath (2.5K). The number of tubules examined in the presence and absence of lumen Na+ is 6 and 4, respectively. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 4 Effects of raising bath K+ from 2.5 to 8.5 mM in the absence and presence of bath EIPA on pHi of the CCD. The luminal Na+ concentration was 0 mM. (a) A typical tracing of pHi before and after raising bath K+ in the absence and presence of bath EIPA; (b) effects of raising bath K+ in the absence of bath EIPA on pHi; and (c) effects of raising bath K+ in the presence of bath EIPA on pHi. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 5 Typical tracings showing effects of raising bath K+ from 2.5 to 8.5 mM in the absence and presence of bath EIPA (100 μM) on VT and VB of the CCD. The luminal Na+ concentration was 0 mM. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 6 Effects of bath EIPA (100 μM) on net K+ secretion (JK) in the presence of lumen Na+ (146.8 mM) or absence of lumen Na+ in response to raising bath K+ from 2.5 to 8.5 mM in the CCD perfused in vitro. Asterisk indicates that under the absence of luminal Na+, JK is significantly lower in the presence of EIPA than in its absence. The number of tubules examined in the absence and presence of bath EIPA with lumen Na+ is 6, whereas that of tubules examined in the absence and presence of bath EIPA without lumen Na+ is 4 and 6, respectively. The tubular flow rates were 6.8–7.0±0.2 nl/min. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 7 Effects of luminal IBX (100 nM) on net K+ secretion (JK) in the presence of lumen Na+ (146.8 mM) or absence of lumen Na+ in response to raising bath K+ from 2.5 to 8.5 mM in the CCD perfused in vitro. Asterisk indicates the significant difference with 8.5 mM K+ in the bath (8.5K) in comparison to 2.5 mM K+ in the bath (2.5K). The number of tubules examined in the absence and presence of lumen IBX with lumen Na+ is 6 and 4, respectively, whereas that of tubules examined in the absence and presence of lumen IBX without lumen Na+ is 4. The tubular flow rates were 6.8–7.0±0.2 nl/min. Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 8 Cell models illustrating the mechanism of K+ secretion in the presence (a) and absence of luminal Na+ transport (b). Note different interactions between the basolateral Na+/H+ exchange and Na+-K+-ATPase in the two experimental conditions (the dotted line indicates a low rate of Na+/H+ exchange). Kidney International 2009 75, 25-30DOI: (10.1038/ki.2008.447) Copyright © 2009 International Society of Nephrology Terms and Conditions