Volume 10, Issue 6, Pages (June 1999)

Slides:



Advertisements
Similar presentations
Reduced Tumor Necrosis Factor-α and Transforming Growth Factor-β1 Expression in the Lungs of Inbred Mice that Fail to Develop Fibroproliferative Lesions.
Advertisements

Mouse Strain Modulates the Role of the Ciliated Cell in Acute Tracheobronchial Airway Injury-Distal Airways  Gregory W. Lawson, Laura S. Van Winkle, Elina.
Lymphatic Vessel Memory Stimulated by Recurrent Inflammation
Characterization of a Novel Necrotic Granuloma Model of Latent Tuberculosis Infection and Reactivation in Mice  Noton K. Dutta, Peter B. Illei, Sanjay.
Etiologic link between sarcoidosis and Propionibacterium acnes
Volume 125, Issue 1, Pages (July 2003)
Athena Kalyvas, Samuel David  Neuron 
Chiara Martinoli, Andrea Chiavelli, Maria Rescigno  Immunity 
Volume 29, Issue 3, Pages (September 2008)
Volume 87, Issue 4, Pages (November 1996)
Volume 28, Issue 2, Pages (February 2008)
IL-22 exacerbates weight loss in a murine model of chronic pulmonary Pseudomonas aeruginosa infection  Hannah K. Bayes, Neil D. Ritchie, Christopher Ward,
Volume 82, Issue 6, Pages (September 2012)
Role of biofilm in Staphylococcus aureus and Staphylococcus epidermidis ventricular assist device driveline infections  Faustino A. Toba, PhD, Hirokazu.
Accelerated Migration of Respiratory Dendritic Cells to the Regional Lymph Nodes Is Limited to the Early Phase of Pulmonary Infection  Kevin L Legge,
Volume 16, Issue 5, Pages (August 2016)
Intracranial Tumor Cell Migration and the Development of Multiple Brain Metastases in Malignant Melanoma  Trude G. Simonsen, Jon-Vidar Gaustad, Einar.
Cheryl Y. Chan, Ashley L. St. John, Soman N. Abraham  Immunity 
Bacterial Sepsis Increases Survival in Metastatic Melanoma: Chlamydophila Pneumoniae Induces Macrophage Polarization and Tumor Regression  Krisztina Buzás,
Volume 41, Issue 3, Pages (September 2014)
Volume 17, Issue 6, Pages (June 2015)
Volume 124, Issue 5, Pages (May 2003)
The NLRP12 Inflammasome Recognizes Yersinia pestis
Pathogenic Escherichia coli in inflammatory bowel diseases
Volume 22, Issue 1, Pages e4 (July 2017)
Soy Isoflavones Promote Radioprotection of Normal Lung Tissue by Inhibition of Radiation-Induced Activation of Macrophages and Neutrophils  Lisa M. Abernathy,
Volume 16, Issue 6, Pages (June 2008)
Volume 24, Issue 5, Pages (May 2006)
Volume 7, Issue 3, Pages (March 2010)
Neorickettsia helminthoeca in Brazilian dogs: a cytopathological, histopathological and immunohistochemical study  S.A. Headley, F.S. Kano, D.G. Scorpio,
Accelerated Migration of Respiratory Dendritic Cells to the Regional Lymph Nodes Is Limited to the Early Phase of Pulmonary Infection  Kevin L Legge,
Tumor Necrosis Factor Signaling Mediates Resistance to Mycobacteria by Inhibiting Bacterial Growth and Macrophage Death  Hilary Clay, Hannah E. Volkman,
Volume 151, Issue 6, Pages e3 (December 2016)
Dynamic Interplay among Monocyte-Derived, Dermal, and Resident Lymph Node Dendritic Cells during the Generation of Vaccine Immunity to Fungi  Karen Ersland,
Volume 130, Issue 2, Pages (February 2006)
Volume 4, Issue 4, Pages (October 2008)
Volume 17, Issue 5, Pages (May 2015)
Volume 29, Issue 2, Pages (August 2008)
Mycobacterium tuberculosis Inhibits Neutrophil Apoptosis, Leading to Delayed Activation of Naive CD4 T cells  Robert Blomgran, Ludovic Desvignes, Volker.
Volume 17, Issue 6, Pages (June 2015)
Volume 17, Issue 2, Pages (February 2015)
Intratracheal IL-13 induces eosinophilic esophagitis by an IL-5, eotaxin-1, and STAT6- dependent mechanism1   Anil Mishra, Marc E Rothenberg  Gastroenterology 
Submucosal territory of the direct lymphatic drainage system to the thoracic duct in the human esophagus  Kenshi Kuge, MD, Gen Murakami, MD, Shunji Mizobuchi,
Volume 13, Issue 4, Pages (October 2000)
Volume 148, Issue 7, Pages (June 2015)
Volume 16, Issue 6, Pages (June 2008)
Volume 44, Issue 1, Pages (January 2016)
Legionella Reveal Dendritic Cell Functions that Facilitate Selection of Antigens for MHC Class II Presentation  Annie L Neild, Craig R Roy  Immunity 
The pho4Δ mutant is less virulent than the WT strain in intranasal (A to C) and intravenous (D to F) models of cryptococcosis. The pho4Δ mutant is less.
Tissue-Resident Memory T Cells
Volume 10, Issue 5, Pages (November 2011)
Interferon-γ-Responsive Nonhematopoietic Cells Regulate the Immune Response to Mycobacterium tuberculosis  Ludovic Desvignes, Joel D. Ernst  Immunity 
Volume 4, Issue 6, Pages (June 2009)
Volume 14, Issue 2, Pages (February 2001)
Tracking Vibrio cholerae Cell-Cell Interactions during Infection Reveals Bacterial Population Dynamics within Intestinal Microenvironments  Yang Fu, Brian.
Volume 127, Issue 5, Pages (November 2004)
Volume 38, Issue 6, Pages (June 2013)
Tumor Necrosis Factor Signaling Mediates Resistance to Mycobacteria by Inhibiting Bacterial Growth and Macrophage Death  Hilary Clay, Hannah E. Volkman,
Melissa B. Uccellini, Adolfo García-Sastre  Cell Reports 
Volume 19, Issue 1, Pages (July 2003)
Role of Apoptosis in Pseudomonas aeruginosa Pneumonia
Volume 4, Issue 3, Pages (September 2008)
Volume 14, Issue 3, Pages (March 2001)
Volume 30, Issue 4, Pages (April 2009)
Volume 62, Issue 1, Pages (July 2002)
Volume 12, Issue 5, Pages (May 2000)
No defect in T-cell priming, secondary response, or tolerance induction in response to inhaled antigens in Fms-like tyrosine kinase 3 ligand–deficient.
Volume 2, Issue 4, Pages (October 2007)
Sang Kyun Ahn, Vanessa Tran, Andrea Leung, Mark Ng, Ming Li, Jun Liu 
Engraftment of Bone Marrow–derived Stem Cells to the Lung in a Model of Acute Respiratory Infection by Pseudomonas aeruginosa  Joanna Rejman, Carla Colombo,
Presentation transcript:

Volume 10, Issue 6, Pages 641-650 (June 1999) The M Cell as a Portal of Entry to the Lung for the Bacterial Pathogen Mycobacterium tuberculosis  Rachel Teitelbaum, William Schubert, Leslie Gunther, Yvonne Kress, Frank Macaluso, Jeffrey W Pollard, David N McMurray, Barry R Bloom  Immunity  Volume 10, Issue 6, Pages 641-650 (June 1999) DOI: 10.1016/S1074-7613(00)80063-1

Figure 1 Interaction of M. tuberculosis with the Surface of Pulmonary M Cells C57BL/6 mice were challenged with 1 × 107 virulent M. tuberculosis, Erdman strain, intratracheally. Tissue was processed for scanning electron microscopy. (A) M. tuberculosis (arrowheads) adhere to and cause a tunneling of the M cell surface (arrows). Bar, 1 μm; magnification, 2,500×. (B) Higher magnification of bacteria in (A), with cluster of M. tuberculosis (arrowhead) adhering to microvilli on the M cell surface (small arrows). Bar, 1 μm; magnification, 10,000×. (C) Higher magnification of bacillus in (A), showing microvilli adherence to bacterial surface as well (arrows). Bar, 1 μm; magnification, 13,000×. Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)

Figure 2 M. tuberculosis Internalization within Pulmonary M Cells and Intraepithelial Leukocytes (A) M. tuberculosis (arrowhead) can be seen adhering to the M cell surface at a region of lymphoid cell accumulation (arrows), by 3 hr post infection. Magnification, 5000×. (B) M. tuberculosis (arrowheads) binds to the M cell surface and is internalized. M. tuberculosis can be seen within M cells in cross section. Magnification, 6000×. (C) M. tuberculosis is internalized within M cells seen here in longitudinal section (arrowhead). Magnification, 9500×. (D) Ultimately, M. tuberculosis (arrowhead) is transcytosed across the M cell surface (smaller arrows) and is found within intraepithelial macrophages (larger arrows denote cell border) Magnification, 8000×. (E) Numerous bacilli (arrowheads) can be found within alveolar macrophages at this time. Magnification, 5000×; bar, 2.5 μm. Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)

Figure 3 Differential Accumulation of Mycobacteria from the Mucosa and the Terminal Alveolus within Their Respective Draining Lymph Nodes Mycobacteria accessing the lymphatic system from the mucosa accumulate more readily in the draining lymph nodes than do bacilli entering the lymphatic system from the terminal alveolus. Normal C57BL/6 mice were infected intranasally with 1 × 107 BCG, and BTLN (filled bars) and PBLN (open bars) were differentially plated for cfu 4 and 24 hr post infection. Data presented as the mean ± SD from two mice harvested in each of three independent experiments. Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)

Figure 4 Mortality and Lung Bacterial Burden in Csfmop/Csfmop Mice Lacking CSF-1 (A) Survival of Csfmop/Csfmop mice (filled circle) (9 mice/group), wild-type controls (filled square) (5 mice/group), and C57BL/6 mice (open diamond) (3 mice/group) challenged intratracheally with 103 virulent M. tuberculosis. (Data are the combination of two separate experiments of Csfmop/Csfmop versus each control.) (B) Number of cfu recovered from the lungs of Csfm/Csfmop (filled square) and C57BL/6 (filled diamond) mice challenged with 103M. tuberculosis (3 mice harvested per group per time point). (C) Csfmop/Csfmop mice produce iNOS in response to M. tuberculosis challenge. Immunohistochemistry of lung tissue from Csfmop/Csfmop mice challenged with 1 × 103M. tuberculosis IT reveals iNOS localized within lung macrophages at 3 weeks post infection (n = 3). (D) Immunohistochemistry for the detection of iNOS in similarly challenged C57Bl/6 mice reveals comparable iNOS production. Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)

Figure 5 Retention of M. tuberculosis within the Intraepithelial Leukocytes of Csfmop/Csfmop Mice 3 Weeks Post Infection Bronchiolar tissue was found to contain acid-fast bacilli (arrowheads) within the epithelia at 3 hr (A) and 3 days (B) post infection in Csfmop/Csfmop mice (magnification, 600×; bar, 25 μm). In contrast, in the C57BL/6 controls, M. tuberculosis (arrowhead) can be seen within lymph vessels (arrow) draining the bronchiole by 24 hr post infection (C) (magnification, 165×). Higher magnification (D) demonstrates several acid-fast bacilli (arrowhead) within the vessel (arrow) (magnification, 600×; bar, 25 μm). Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)

Figure 6 Csfmop/Csfmop Mice Retain Bacilli within the Mucosa Early after Infection, Preventing Deposition within the Draining Lymph Nodes (A) M. tuberculosis is retained within the Csfmop/Csfmop mucosa, as compared to controls, early after infection. C57Bl/6 mice (black bars) and Csfmop/Csfmop mice (white bars) were challenged with 1 × 107M. tuberculosis IN. At 36 hr post infection, lungs and airway were removed, and mucosal tissue was microdissected away from lung parenchyma, homogenized, and plated for cfu. Data are pools of tissue from three animals per group, presented as mean ± SD, and were evaluated by the Student’s t test (p ≤ 0.0001). (B) M. tuberculosis fails to access the draining lymph nodes 36 hr post infection, at a time when they are retained within the mucosa. BTLN and PBLN from C57Bl/6 mice (black bars) and Csfmop/Csfmop mice (white bars) were harvested 36 hr post infection and plated for cfu (n = 3) (p ≤ 0.001). Immunity 1999 10, 641-650DOI: (10.1016/S1074-7613(00)80063-1)