Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity.

Slides:

Advertisements

Similar presentations

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

Advertisements

Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

Molecular phenotyping of HCS-2/8 cells as an in vitro model of human chondrocytes J. Saas, Ph.D., K. Lindauer, Ph.D., B. Bau, M.Sc., M. Takigawa, D.D.S.,

The disease modifying osteoarthritis drug diacerein is able to antagonize pro inflammatory state of chondrocytes under mild mechanical stimuli B. Steinecker-Frohnwieser,

M. Fu, G. Huang, Z. Zhang, J. Liu, Z. Zhang, Z. Huang, B. Yu, F. Meng

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Chondro-protective effects of low intensity pulsed ultrasound

Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress S.-C. Su,

BMP-2 induces the expression of chondrocyte-specific genes in bovine synovium- derived progenitor cells cultured in three-dimensional alginate hydrogel

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

Effective reduction of the interleukin-1β transcript in osteoarthritis-prone guinea pig chondrocytes via short hairpin RNA mediated RNA interference influences.

Expression of superficial zone protein in mandibular condyle cartilage

Insulin-like growth factor-1 boosts the developing process of condylar hyperplasia by stimulating chondrocytes proliferation Y. Chen, J. Ke, X. Long,

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Trichostatin A, a histone deacetylase inhibitor, suppresses synovial inflammation and subsequent cartilage destruction in a collagen antibody-induced.

M. -H. Moon, J. -K. Jeong, Y. -J. Lee, J. -W. Seol, C. J. Jackson, S

Potential roles of cytokines and chemokines in human intervertebral disc degeneration: interleukin-1 is a master regulator of catabolic processes K.L.E.

A. R. Klatt, B. Paul-Klausch, G. Klinger, U. Hillebrand, G. Kühn, B

MicroRNA221-3p modulates Ets-1 expression in synovial fibroblasts from patients with osteoarthritis of temporomandibular joint J. Xu, Y. Liu, M. Deng,

Inhibition of cyclooxygenase 2 expression by diallyl sulfide on joint inflammation induced by urate crystal and IL-1β H.-S. Lee, M.D., Ph.D., C.-H. Lee,

Role of hypoxia-inducible factor-1 alpha in the regulation of plasminogen activator activity in rat knee joint chondrocytes G. Zhu, Y. Tang, X. Liang,

TGFβ inhibition during expansion phase increases the chondrogenic re-differentiation capacity of human articular chondrocytes R. Narcisi, L. Signorile,

The identification of differentially expressed microRNA in osteoarthritic tissue that modulate the production of TNF-α and MMP13 S.W. Jones, Ph.D., G.

Nitric oxide enhances aggrecan degradation by aggrecanase in response to TNF-α but not IL-1β treatment at a post-transcriptional level in bovine cartilage.

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Long-term NSAID treatment directly decreases COX-2 and mPGES-1 production in the articular cartilage of patients with osteoarthritis M.A. Álvarez-Soria,

L. Raymond, S. Eck, E. Hays, I. Tomek, M. D. , S. Kantor, M. D. , M

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes S.J. Park, E.J. Cheon,

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes N. Chabane, M.Sc.,

Anti-apoptotic effect of transforming growth factor-β1 on human articular chondrocytes: role of protein phosphatase 2A M. Lires-Deán, B.S., B. Caramés,

M.A. Greene, R.F. Loeser Osteoarthritis and Cartilage

Identification of synovial fluid microRNA signature in knee osteoarthritis: differentiating early- and late-stage knee osteoarthritis Y.-H. Li, G. Tavallaee,

M. Akagi, M. D. , Ph. D. , A. Ueda, M. D. , T. Teramura, Ph. D. , S

MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1β-induced chondrocyte responses F. Meng, Z. Zhang, W.

Differential expression of leptin and leptin's receptor isoform (Ob-Rb) mRNA between advanced and minimally affected osteoarthritic cartilage; effect.

Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis.

Phenotype-related differential α-2,6- or α-2,3-sialylation of glycoprotein N-glycans in human chondrocytes S. Toegel, M. Pabst, S.Q. Wu, J. Grass, M.B.

M. E. R. van Meegeren, G. Roosendaal, N. W. D. Jansen, M. J. G

Expression and regulation of Toll-like receptor 2 by IL-1β and fibronectin fragments in human articular chondrocytes S.-L. Su, M.S., C.-D. Tsai, Ph.D.,

Interleukin-1β and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation

Osteoblasts from the sclerotic subchondral bone downregulate aggrecan but upregulate metalloproteinases expression by chondrocytes. This effect is mimicked.

K. Fundel, Ph. D. , J. Haag, Ph. D. , P. M. Gebhard, M. Sc. , R

Distinctive pro-inflammatory gene signatures induced in articular chondrocytes by oncostatin M and IL-6 are regulated by Suppressor of Cytokine Signaling-3

DIO2 modifies inflammatory responses in chondrocytes

Expression of proteinase-activated receptors (PAR)-2 in articular chondrocytes is modulated by IL-1β, TNF-α and TGF-β Y. Xiang, M.D., K. Masuko-Hongo,

Isoliquiritigenin Inhibits IL-1β-Induced Production of Matrix Metalloproteinase in Articular Chondrocytes Lei Zhang, Shiyun Ma, Hang Su, Jiaxiang Cheng

Salubrinal reduces expression and activity of MMP13 in chondrocytes

Selenomethionine inhibits IL-1β inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) expression in primary human chondrocytes A.W.M. Cheng,

Dr. M. C. Honorati, Ph. D. , S. Neri, Ph. D. , L. Cattini, B. Sc. , A

Methylation of the OP-1 promoter: potential role in the age-related decline in OP-1 expression in cartilage R.F. Loeser, M.D., H.-J. Im, Ph.D., B. Richardson,

Inflammatory stimuli differentially modulate the transcription of paracrine signaling molecules of equine bone marrow multipotent mesenchymal stromal.

Dr J. Deschner, D. M. D. , Ph. D. , Dr B. Rath-Deschner, D. M. D. , Ph

Mitogen-activated protein kinase-activated protein kinase 2 (MK2) modulates key biological pathways associated with OA disease pathology S.W. Jones,

J. Ranstam Osteoarthritis and Cartilage

Z. Lin, M. B. , N. J. Pavlos, B. Sc. (Hons. ), Ph. D. , M. A. Cake, B

Glutamate signaling in chondrocytes and the potential involvement of NMDA receptors in cell proliferation and inflammatory gene expression T. Piepoli,

Activation by IL-1 of bovine articular chondrocytes in culture within a 3D collagen-based scaffold. An in vitro model to address the effect of compounds.

Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells T. Tetsunaga,

Mevastatin reduces cartilage degradation in rabbit experimental osteoarthritis through inhibition of synovial inflammation Y. Akasaki, M.D., S. Matsuda,

Synergistic effect of chondroitin sulfate and cyclic pressure on biochemical and morphological properties of chondrocytes from articular cartilage G.

Spingosine-1-phosphate stimulates proliferation and counteracts interleukin-1 induced nitric oxide formation in articular chondrocytes M.H. Stradner,

Comparative effects of IL-1β and hydrogen peroxide (H2O2) on catabolic and anabolic gene expression in juvenile bovine chondrocytes G. Martin, Ph.D.,

Chondroitin sulfate modulation of matrix and inflammatory gene expression in IL-1β- stimulated chondrocytes – study in hypoxic alginate bead cultures

Celecoxib exerts protective effects on extracellular matrix metabolism of mandibular condylar chondrocytes under excessive mechanical stress S.-C. Su,

PTHrP overexpression partially inhibits a mechanical strain-induced arthritic phenotype in chondrocytes D. Wang, J.M. Taboas, R.S. Tuan Osteoarthritis.

Dynamic compression of single cells

Expression of superficial zone protein in mandibular condyle cartilage

Chondro-protective effects of low intensity pulsed ultrasound

IGF-1 regulation of type II collagen and MMP-13 expression in rat endplate chondrocytes via distinct signaling pathways M. Zhang, Ph.D., Q. Zhou, M.D.,

Presentation transcript:

Comparison of the chondrosarcoma cell line SW1353 with primary human adult articular chondrocytes with regard to their gene expression profile and reactivity to IL-1β M. Gebauer, Ph.D., J. Saas, Ph.D., F. Sohler, M.Sc., J. Haag, M.Sc., S. Söder, M.D., M. Pieper, Ph.D., E. Bartnik, Ph.D., J. Beninga, Ph.D., R. Zimmer, Ph.D., T. Aigner, M.D., D.Sc. Osteoarthritis and Cartilage Volume 13, Issue 8, Pages 697-708 (August 2005) DOI: 10.1016/j.joca.2005.04.004 Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 1 Two-dimensional hierarchical clustering of hybridization experiments from human SW1353 cells and PHCs stimulated in triplicate with 1ng/ml IL-1β at 30min, 6h, 16h, 24h and 48h. For the five time intervals of IL-1β stimulation, expression profiles of treated samples were compared to control samples (triplicates each). Dendrograms illustrate SW1353 expression values (a) and PHCs expression values (b) with genes that were significantly regulated by IL-1β in SW1353 cells (1.2× absolute fold-change and P-value<0.05 in at least two time points). Dendrograms (c) and (d) show the corresponding expression data for genes that were significantly regulated by IL-1β in PHCs (same criteria as above). Osteoarthritis and Cartilage 2005 13, 697-708DOI: (10.1016/j.joca.2005.04.004) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 2 Real-time PCR analysis of the IL-1β induced expression of IL-6 and LIF (a) and MMP-1, -3, and -13 (b) in SW1353 cells (solid bars) and PHCs (open bars) after 48h of stimulation with 1ng IL-1β (SW1353: three independent experiments; PHCs: six independent experiments). Osteoarthritis and Cartilage 2005 13, 697-708DOI: (10.1016/j.joca.2005.04.004) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 3 Representation of protease levels (a: MMP-1; b: MMP-3; c: MMP-13) secreted into the culture medium by SW1353 cells without and with stimulation by 0.1ng/ml IL-1β (for 24h). Total protease levels (i.e., active and pro-forms) were measured by the ELISA (given as ng/ml medium). Osteoarthritis and Cartilage 2005 13, 697-708DOI: (10.1016/j.joca.2005.04.004) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 4 Expression profiles of genes in the predicted NFκB regulation context (the only regulation context common to both systems according to our analysis) as log-ratios of expression levels between IL-1β stimulated cells and untreated cells against time in hours after the treatment. Profiles for SW1353 cells are shown as dashed lines, profiles for PHCs as solid lines. Correlation values quantify the similarity between the two systems for each gene. Statistical analysis showed that the correlation of the NFκB targets is significantly higher than expected by chance (details are given in the text). Osteoarthritis and Cartilage 2005 13, 697-708DOI: (10.1016/j.joca.2005.04.004) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions

Fig. 5 Expression profiles of genes in the predicted ATF2 regulation context as log-ratios of expression levels between IL-1β stimulated cells and untreated cells against time in hours after the treatment. Profiles for SW1353 cells are shown as dotted lines, profiles for PHCs as drawn lines. Correlation values quantify the similarity between the two systems for each gene. Statistical analysis showed that the correlation of the ATF2 targets is not significant (details are given in the text). (*The down-regulation of TNF-alpha after 16h in PHCs might represent a calculation error due to very low expression levels detected.) Osteoarthritis and Cartilage 2005 13, 697-708DOI: (10.1016/j.joca.2005.04.004) Copyright © 2005 OsteoArthritis Research Society International Terms and Conditions