G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage

Slides:

Advertisements

Similar presentations

Volume 49, Issue 1, Pages (January 2006)

Advertisements

The effect of platelet-rich plasma on the regenerative therapy of muscle derived stem cells for articular cartilage repair Y. Mifune, T. Matsumoto, K.

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

CXC chemokine ligand 12a enhances chondrocyte proliferation and maturation during endochondral bone formation G.-W. Kim, M.-S. Han, H.-R. Park, E.-J.

Expression pattern differences between osteoarthritic chondrocytes and mesenchymal stem cells during chondrogenic differentiation P. Bernstein, C. Sticht,

Volume 8, Issue 3, Pages (March 2017)

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

CCN family 2/connective tissue growth factor (CCN2/CTGF) stimulates proliferation and differentiation of auricular chondrocytes T. Fujisawa, Ph.D., D.D.S.,

Volume 49, Issue 1, Pages (January 2006)

Immortalization and characterization of mouse temporomandibular joint disc cell clones with capacity for multi-lineage differentiation Y. Park, J. Hosomichi,

Muscle cell-derived factors inhibit inflammatory stimuli-induced damage in hMSC- derived chondrocytes R.S. Rainbow, H. Kwon, A.T. Foote, R.C. Preda, D.L.

GDF5 reduces MMP13 expression in human chondrocytes via DKK1 mediated canonical Wnt signaling inhibition L. Enochson, J. Stenberg, M. Brittberg, A. Lindahl

Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal.

Regulation of mesenchymal stem cell chondrogenesis by glucose through protein kinase C/transforming growth factor signaling T.-L. Tsai, P.A. Manner,

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

MicroRNA-92a-3p regulates the expression of cartilage-specific genes by directly targeting histone deacetylase 2 in chondrogenesis and degradation G.

Combination of ADMSCs and chondrocytes reduces hypertrophy and improves the functional properties of osteoarthritic cartilage M.R. Ahmed, A. Mehmood,

Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus T. Nishida,

Synovial mesenchymal stem cells from osteo- or rheumatoid arthritis joints exhibit good potential for cartilage repair using a scaffold-free tissue engineering.

Hypoxia reduces the inhibitory effect of IL-1β on chondrogenic differentiation of FCS- free expanded MSC T. Felka, R. Schäfer, B. Schewe, K. Benz, W.K.

Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao

Intermittent cyclic mechanical tension promotes endplate cartilage degeneration via canonical Wnt signaling pathway and E-cadherin/β-catenin complex cross-talk

Hypertrophic differentiation during chondrogenic differentiation of progenitor cells is stimulated by BMP-2 but suppressed by BMP-7 M.M.J. Caron, P.J.

X. Zhang, I. Prasadam, W. Fang, R. Crawford, Y. Xiao

Adipose-derived stem cells induce autophagic activation and inhibit catabolic response to pro-inflammatory cytokines in rat chondrocytes Li-Bo Jiang,

Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage D. Ikegami, T.

R. Piva, E. Lambertini, C. Manferdini, C. Capanni, L. Penolazzi, E

J.E. Lafont, F.-A. Poujade, M. Pasdeloup, P. Neyret, F. Mallein-Gerin

Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell- engineered tissue constructs M. Pei, F. He, B.M. Boyce, V.L.

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

HIF-1α as a Regulator of BMP2-Induced Chondrogenic Differentiation, Osteogenic Differentiation, and Endochondral Ossification in Stem Cells Cell Physiol.

C.B. Chang, S.A. Han, E.M. Kim, S. Lee, S.C. Seong, M.C. Lee

MicroRNA-320 regulates matrix metalloproteinase-13 expression in chondrogenesis and interleukin-1β-induced chondrocyte responses F. Meng, Z. Zhang, W.

Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture Dr R.L. Mauck, Ph.D., X. Yuan, Dr.

Exaggerated inflammatory environment decreases BMP-2/ACS-induced ectopic bone mass in a rat model: implications for clinical use of BMP-2 R.-L. Huang,

S. Varghese, Ph. D. , P. Theprungsirikul, B. S. , S. Sahani, B. S. , N

CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFβ and BMP through SMAD signaling B. Saitta,

Toward scaffold-based meniscus repair: effect of human serum, hyaluronic acid and TGF-ß3 on cell recruitment and re-differentiation U. Freymann, M. Endres,

Comparative analysis with collagen type II distinguishes cartilage oligomeric matrix protein as a primary TGFβ-responsive gene H. Li, D.R. Haudenschild,

Glucosamine promotes chondrogenic phenotype in both chondrocytes and mesenchymal stem cells and inhibits MMP-13 expression and matrix degradation A.

The immunosuppressant FK506 promotes development of the chondrogenic phenotype in human synovial stromal cells via modulation of the Smad signaling pathway

H.H. Lee, M.J. O'Malley, N.A. Friel, C.R. Chu

V. Queirolo, D. Galli, E. Masselli, R. M. Borzì, S. Martini, F

Transcription factor SPB-x is a key molecule inducing hypertrophy of differentiated chondrocyte from MSC G.-I. Im, J.-M. Lee, J.-M. Ahn, E.-A. Kim Osteoarthritis.

Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that.

M.M.-G. Sun, F. Beier Osteoarthritis and Cartilage

Involvement of Gas7 along the ERK1/2 MAP kinase and SOX9 pathway in chondrogenesis of human marrow-derived mesenchymal stem cells Y. Chang, M.D., S.W.N.

Repair of full-thickness femoral condyle cartilage defects using allogeneic synovial cell- engineered tissue constructs M. Pei, F. He, B.M. Boyce, V.L.

Single cell sorting identifies progenitor cell population from full thickness bovine articular cartilage Y. Yu, H. Zheng, J.A. Buckwalter, J.A. Martin

Photo-crosslinked alginate hydrogels support enhanced matrix accumulation by nucleus pulposus cells in vivo A.I. Chou, S.O. Akintoye, S.B. Nicoll Osteoarthritis.

Synovial mesenchymal stem cells from osteo- or rheumatoid arthritis joints exhibit good potential for cartilage repair using a scaffold-free tissue engineering.

A predominantly articular cartilage-associated gene, SCRG1, is induced by glucocorticoid and stimulates chondrogenesis in vitro Kensuke Ochi, M.D., Ph.D.,

Enhancing and maintaining chondrogenesis of synovial fibroblasts by cartilage extracellular matrix protein matrilins M. Pei, M.D., Ph.D., J. Luo, M.D.,

Lentiviral vector-mediated shRNAs targeting a functional isoform of the leptin receptor (Ob-Rb) inhibit cartilage degeneration in a rat model of osteoarthritis

N.D. Miljkovic, M.D., Ph.D., G.M. Cooper, Ph.D., K.G. Marra, Ph.D.

Autologous chondrocyte implantation (ACI) for aged patients: development of the proper cell expansion conditions for possible therapeutic applications

Magnesium enhances adherence and cartilage formation of synovial mesenchymal stem cells through integrins M. Shimaya, T. Muneta, S. Ichinose, K. Tsuji,

Do adipose tissue-derived mesenchymal stem cells have the same osteogenic and chondrogenic potential as bone marrow-derived cells? Gun-II Im, M.D., Yong-Woon.

Regulation of senescence associated signaling mechanisms in chondrocytes for cartilage tissue regeneration S. Ashraf, B.-H. Cha, J.-S. Kim, J. Ahn, I.

Development of growth factor tethered hyaluronan microspheres for in situ chondrogenic differentiation of human mesenchymal stem cells S. Ansboro, J.S.

L. De Franceschi, Ph. D. , L. Roseti, Ph. D. , G. Desando, Ph. D. , A

A peptide temporally enhanced chondrogenesis of mesenchymal stem cells

Membrane culture and reduced oxygen tension enhances cartilage matrix formation from equine cord blood mesenchymal stromal cells in vitro C. Co, M.K.

Optimal ratio of adipose stem cells and bone marrow stem cell to promote osteogenic differentiation and angiogenesis G.-I. Im, K.-I. Kim, N.-H. Jo Osteoarthritis.

Enhanced phagocytic capacity endows chondrogenic progenitor cells with a novel scavenger function within injured cartilage C. Zhou, H. Zheng, J.A. Buckwalter,

Chondro-protective effects of low intensity pulsed ultrasound

K. -C. Wang, E. Kwan, K. Aris, T. T. Egelhoff, A. I. Caplan, J. F

Inhibition of miR-449a Promotes Cartilage Regeneration and Prevents Progression of Osteoarthritis in In Vivo Rat Models Dawoon Baek, Kyoung-Mi Lee, Ki.

Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy M.E. Cooke,

Effect of expansion medium on ex vivo gene transfer and chondrogenesis in type II collagen–glycosaminoglycan scaffolds in vitro R.M. Capito, Ph.D., M.

Presentation transcript:

Electroporation-mediated gene transfer of SOX trio to enhance chondrogenesis in adipose stem cells G.-I. Im, H.-J. Kim Osteoarthritis and Cartilage Volume 19, Issue 4, Pages 449-457 (April 2011) DOI: 10.1016/j.joca.2011.01.005 Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Transfection was demonstrated by fluorescence microscopy. ASCs transfected with pEGFP-C1 without interposed gene, SOX-5, SOX-6, SOX-9, and all three genes (SOX trio): 24h after transfection (A) and 21 days after transfection and pellet culture (B). 4',6-diamidino-2-phenylindole (DAPI) staining (blue, indicating nuclei) was merged with green fluorescent protein (GFP) fluorescent imaging (green). Osteoarthritis and Cartilage 2011 19, 449-457DOI: (10.1016/j.joca.2011.01.005) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Transfection of SOX-5, -6, and -9 elevated mRNA and protein expression of transfected gene. Gene (A) and protein (B) expression of SOX-5, SOX-6, and SOX-9 in ASCs transfected with pEGFP-C1 without interposed gene (vector), SOX-5, SOX-6, SOX-9, and all three genes (SOX-5, -6, and -9), and in ASCs treated with TGF-β2 and BMP-7 (TGF+BMP) after 21 days of in vitro culture. N=3, CI: confidence interval. Osteoarthritis and Cartilage 2011 19, 449-457DOI: (10.1016/j.joca.2011.01.005) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 DNA level was unaffected while GAG was elevated by SOX trio co-transfection in ASCs. DNA levels (A), GAG levels normalized to DNA (B), and Safranin-O staining of ASCs (C) transfected with pEGFP-C1 without interposed gene (vector), SOX-5, SOX-6, SOX-9, and all three genes (SOX trio), and in ASCs treated with TGF-β2 and BMP-7 (TGF+BMP) after 21 days of in vitro culture. N=5, CI: confidence interval. Osteoarthritis and Cartilage 2011 19, 449-457DOI: (10.1016/j.joca.2011.01.005) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Chondrogenic marker genes and proteins were enhanced by SOX trio co-transfection. Gene expression of COL2A1, COL1A1, and COL10A1 (A), Western blotting for type II collagen, type I collagen and type X collagen (B) in ASCs transfected with pEGFP-C1 without interposed gene (vector), SOX-5, SOX-6, SOX-9, and all three genes (SOX trio), and in ASCs treated with TGF-β2 and BMP-7 (TGF+BMP) after 21 days of in vitro culture. N=3, CI: confidence interval. Osteoarthritis and Cartilage 2011 19, 449-457DOI: (10.1016/j.joca.2011.01.005) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Persistent proteoglycan and reduced calcification were observed in in-vivo transplanted ASCs co-transfected with SOX trio. Pellets were implanted in subcutaneous pockets of SCID mice for 3 weeks: Safranin-O staining and Alizarin red staining in ASCs transfected with pEGFP-C1 without interposed gene (vector), all three genes (SOX trio). Scale bars are equal to 500μm (A). The results of GAG assay and calcium assay are also presented (B). Osteoarthritis and Cartilage 2011 19, 449-457DOI: (10.1016/j.joca.2011.01.005) Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions