In vivo gene gun–mediated transduction into rat heart with Epstein-Barr virus-based episomal vectors Kazuhiko Nishizaki, MD, Osam Mazda, MD, PhD, Yoshiko.

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In vivo gene gun–mediated transduction into rat heart with Epstein-Barr virus-based episomal vectors Kazuhiko Nishizaki, MD, Osam Mazda, MD, PhD, Yoshiko Dohi, PhD, Tetsuji Kawata, MD, Kazumi Mizuguchi, MD, Soichiro Kitamura, MD, Shigeki Taniguchi, MD The Annals of Thoracic Surgery Volume 70, Issue 4, Pages 1332-1337 (October 2000) DOI: 10.1016/S0003-4975(00)01708-2

Fig 1 Plasmids used in this study. Maps of pSES.β (left) and pS.β (right) are shown. (Prom = promoter, polyA = SV40 polyA additional signal.) The Annals of Thoracic Surgery 2000 70, 1332-1337DOI: (10.1016/S0003-4975(00)01708-2)

Fig 2 X-gal staining of the bombarded heart. Rat hearts were bombarded with 1.0 Au (A, B, E, and F) or 1.5 Au (C, D, G, and H) gold particles coated with pSES.β (A, C, E, and G) or pS.β (B, D, F, and H). Seven days (A–D) or 4 weeks (E–H) later, rats were sacrificed and the hearts were excised. The hearts were cut into pieces and subjected to X-gal staining as described in Material and Methods. The bar indicates 500 μm. The Annals of Thoracic Surgery 2000 70, 1332-1337DOI: (10.1016/S0003-4975(00)01708-2)

Fig 3 Microscopic observation of bombarded heart stained with X-gal. Rats hearts were bombarded with 1.0 Au (A and B) or 1.5 Au (C and D) gold particles coated with pSES.β (A and C) or pS.β (B and D), and 3 weeks later, rats were sacrificed and the hearts were excised. The heart sections were subjected to X-gal staining and Kernechtrot staining so that nucleus stained pink. The arrow indicates gold particle. Magnification × 400, A–C; × 200, D. The Annals of Thoracic Surgery 2000 70, 1332-1337DOI: (10.1016/S0003-4975(00)01708-2)

Fig 4 Polymerase chain reaction (PCR) analyses of DNA from various organs. DNA was prepared from the lung (lane 1), brain (lane 2), liver (lane 3), spleen (lane 4), or kidney (lane 5) of a rat whose heart was bombarded with pSES.β/1.0 Au. DNA was also prepared from hearts bombarded with pS.β/1.5 Au (lane 6), pSES.β/1.5 Au (lane 7), pS.β/1.0 Au (lane 8), pSES.β/1.0 Au (lane 9), or 1.0 Au alone (lane 10). As positive controls, 5 ng pS.β (lane 11) and pSES.β (lane 12) were also tested. PCR was performed using β-gal specific primers as described in Material and Methods. (M = molecular weight marker.) The Annals of Thoracic Surgery 2000 70, 1332-1337DOI: (10.1016/S0003-4975(00)01708-2)