Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII

Slides:

Advertisements

Similar presentations

Online supplementary material. Profound and persistent decrease of circulating dendritic cells is associated with ICU-acquired infection in patients with.

Advertisements

IMMUNOPHENOTYPING OF HEMATOPOIETIC PROGENITOR CELLS BY MULTIPARAMETER FLOW CYTOMETRY.

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.

Supplemental Figure 1 A. EpCAM Gating Strategy Top Row: Histogram showing unstained BT474 cells with low fluorescent intensity of the EpCAM specific PE.

Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

Figure S1 (A) Analysis of purified myeloid DCs and T cells. Freshly isolated mDCs were processed for cytospins (A), stained with haematoxylin-eosin (x400.

CD44 PD-L1 FSC Human/Mouse Stromal Markers FSC DAPI FSC SSC FSC-W FSC-H SSC-W SSC-H Live cells Tumor cells CD44 - CD44 + Supplementary Figure S1. Gating.

AB C D E F G H Supplemental Fig. 1. Figure S1. MIF induces maturation of CD11b + but not CD8α + DCs in spleen from Mif -/- mice infected with T. gondii.

Figure 1 WBC count (A), platelet count (B), MPV (C), platelet activation rate (D) and TGF-β1concentration (E) in the PF of women with endometriosis and.

SSC -A SLAN CD16 CD14 CD3-CD19-CD56 CD123 HLA-DR CD33

D. Negative fraction after isolation of

Multiparametric flow cytometry of normal human bone marrow:

mTECs produce tissue restricted antigens (TRAs)

Plot 4: A01 P0 CHO Count Events / μL % of This Plot % of All Mean FSC-A Mean SSC-A CV FSC-A CV SSC-A Median FSC-A Median SSC-A All 8,

Foxp3+ Regulatory T Cells and Natural Killer Cells Distinctly Infiltrate Primary Tumors and Draining Lymph Nodes in Pulmonary Adenocarcinoma Thomas Schneider,

Image 1 Detection of minimal residual disease (MRD) in consecutive bone marrow (BM) samples from a patient with relapse (A) and a patient still in remission.

Figure 2 Percentage of CD19+/CD34+ cells with decreased CD81 median fluorescence intensity (MFI;

Invest. Ophthalmol. Vis. Sci ;49(4): doi: /iovs Figure Legend:

Supplementary Figure 1 CD45 CD14 FSC CD123 HLA-DR CD11c Lineage

Supplementary Figure S1

COMPENSATION + FITC - PE - + PE - FITC - FITC detector FITC PE

Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor Preston E. Bratcher, Steven M. Rowe, Ginger.

Human group 2 innate lymphoid cells do not express the IL-5 receptor

A B C D CD4+ CD8+ Control Isotype Ab Anti-TNFa Ab Control Isotype Ab

by Venetia Bigley, Laura E. Spence, and Matthew Collin

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Recipient-Derived Cells after Cord Blood Transplantation: Dynamics Elucidated by Multicolor FACS, Reflecting Graft Failure and Relapse Nobukazu Watanabe,

Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3 Thomas Schneider,

A B Supplemental Figure 1 PBMC Cervical Cytobrush

Identification of a distinct glucocorticosteroid-insensitive pulmonary macrophage phenotype in patients with chronic obstructive pulmonary disease Kirandeep.

Type 3 innate lymphoid cells induce proliferation of CD94+ natural killer cells Shuo Li, PhD, Hideaki Morita, MD, PhD, Beate Rückert, Sci Tec, Tadech.

A B C D E F Supplementary Fig. 1. Gating strategy for CD44+CD133+CD24+ B16 tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells.

A B C D E F Supplementary Fig. 2. Gating strategy for CD44+CD24+ ID8agg tumor initiating cells (TIC). Cells were gated on CD44. CD44 positive cells were.

Depletion of CD169+ cells leads to increased expression of CD25 on enterotoxin A–specific Vβ3+ T cells. Depletion of CD169+ cells leads to increased expression.

Responsiveness to respiratory syncytial virus in neonates is mediated through thymic stromal lymphopoietin and OX40 ligand Junyan Han, PhD, Azzeddine.

Frederic Geissmann, Steffen Jung, Dan R. Littman Immunity

Cord Blood Units with Low CD34+ Cell Viability Have a Low Probability of Engraftment after Double Unit Transplantation Andromachi Scaradavou, Katherine.

Identification of a distinct glucocorticosteroid-insensitive pulmonary macrophage phenotype in patients with chronic obstructive pulmonary disease Kirandeep.

Deleted in Azoospermia-Like (DAZL) gene–expressing cells in human amniotic fluid: a new source for germ cells research? Konstantinos Stefanidis, M.D.,

Foxp3+ Regulatory T Cells and Natural Killer Cells Distinctly Infiltrate Primary Tumors and Draining Lymph Nodes in Pulmonary Adenocarcinoma Thomas Schneider,

Human Treg cells express PlexinB1.

Flow cytometric analysis of putative progenitor cells.

Increased chemokine content and leukocyte infiltrate in D6-negative tumors. Increased chemokine content and leukocyte infiltrate in D6-negative tumors.

Supplementary Figure 1 FSC-A SSC-A HC UC CD

Label-Retaining Cells (Presumptive Stem Cells) of Mice Vibrissae Do Not Express Gap Junction Protein Connexin 43 Maja Matic, Marcia Simon Journal of.

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Identification of TROP2 (TACSTD2), an EpCAM-Like Molecule, as a Specific Marker for TGF-β1-Dependent Human Epidermal Langerhans Cells Gregor Eisenwort,

Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique Giuseppe Ricci, M.D., Sandra Perticarari, B.Sc.,

Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3 Thomas Schneider,

Flow cytometric analysis of cytokine production by lymphocytes in the peripheral blood (PB) and in joints. Paired samples of lymphocytes prepared from.

Figure S1. Representative gating of 7-hr suppression assay from one subject Teff with media alone (top row), Teff with activation beads but not Tregs (middle),

Supplemental Digital Content 2 – Figure 1

Refer to word document for this table

CD24a Expression Levels Discriminate Langerhans Cells from Dermal Dendritic Cells in Murine Skin and Lymph Nodes Susanne Stutte, Bettina Jux, Charlotte.

d a c b CD8 CD3 CD4 CD62L HLA-DR CD57 10% 85% 11% 76% 19% 60% 36% 66%

Supplemental Digital Content 3 – Figure 2

Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression Kathryn E. Hulse, PhD, James E.

Murine Epidermal Label-Retaining Cells Isolated by Flow Cytometry do not Express the Stem Cell Markers CD34, Sca-1, or Flk-1 Michael R. Albert, Ruth-Ann.

CellTrace labeling of primary OT-I T cells allows for identification of OT-I nuclei after coculture. CellTrace labeling of primary OT-I T cells allows.

CD127low/- and FoxP3+ Expression Levels Characterize Different Regulatory T-Cell Populations in Human Peripheral Blood Sandra Klein, Cosima C. Kretz,

CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to.

Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.

Flow cytometry of subcutaneous adipose tissue stromovascular cells.

Plasmacytoid Dendritic Cells: A New Cutaneous Dendritic Cell Subset with Distinct Role in Inflammatory Skin Diseases Andreas Wollenberg, Sandra Günther,

Induction of IL-3–secreting CD4+ T cells.

Variable CD7 Expression on T Cells in the Leukemic Phase of Cutaneous T Cell Lymphoma (Sézary Syndrome) Eric C. Vonderheid, Amy Kotecha, Christine M.

ODM-203 administration results in immune activation in a syngenic model. ODM-203 administration results in immune activation in a syngenic model. Effect.

The Majority of Epidermal T Cells in Psoriasis Vulgaris Lesions can Produce Type 1 Cytokines, Interferon-γ, Interleukin-2, and Tumor Necrosis Factor-α,

Supplementary Figures

Presentation transcript:

Figure S2 Gating strategy of AT2 cells based on EpCAM and MHCII 84.8 FSC SSC Live cells 86.6 7-AAD FSC singlet 95.5 FSC-Hight FSC-Width SSC singlet 92.0 SSC-Height SSC-Width CD45-CD31- cells 28.1 CD45,CD31-PE-Cy7 RatIgG2bκ,RatIgG2aκ-PE-Cy7 RatIgG2aκ-APC RatIgG2bκ-eFluor450 EpCAM-APC P1 89.3 P2 1.6 P3 2.6 MHCII-eFluor450 Figure S2 Gating strategy for isolation of AT2 cells based on EpCAM and MHCII expression Gating strategy for isolation of AT2 cells from single cell suspension of the lungs is demonstrated. Gating of each cell population was performed along the order of arrows. Dotted arrows indicate plots with relevant isotype controls.