Diverging signaling events control the pathway of GPVI down-regulation in vivo by Tamer Rabie, David Varga-Szabo, Markus Bender, Rastislav Pozgaj, Francois.

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Diverging signaling events control the pathway of GPVI down-regulation in vivo by Tamer Rabie, David Varga-Szabo, Markus Bender, Rastislav Pozgaj, Francois Lanza, Takashi Saito, Stephen P. Watson, and Bernhard Nieswandt Blood Volume 110(2):529-535 July 15, 2007 ©2007 by American Society of Hematology

GPVI down-regulation through ectodomain shedding. GPVI down-regulation through ectodomain shedding. Wild-type (•) or FcRγ−/− (○) mice received 20 μg biotinylated JAQ1 intravenously and (A) platelet counts were determined. (B) Surface-bound JAQ1 was detected by flow cytometric measurement of streptavidinFITC binding after 10 minutes (black line) and 30 minutes (dotted line). The shaded area represents maximal binding (t = 0 minutes) as determined after in vitro incubation of platelets with 20 μg/mL JAQ1biotin followed by streptavidinFITC. (C) Detection of sGPVI/JAQ1biotin complex in plasma of wild-type (•) or FcRγ−/− (○) mice at the indicated time points after antibody injection. (D) Western blot analysis of platelets at the indicated time points. (A,C) Results are expressed as the mean platelet count ± SD for groups of 6 mice each. (B,D) The results are representative of 3 individual experiments. Tamer Rabie et al. Blood 2007;110:529-535 ©2007 by American Society of Hematology

Signaling through the FcRγ-ITAM is essential for all JAQ1-induced effects. Signaling through the FcRγ-ITAM is essential for all JAQ1-induced effects. Wild-type (•) or FcRγ-YF (○) mice received 20 μg biotinylated JAQ1 intravenously and (A) platelet counts were determined. (B) Surface-bound JAQ1biotin was detected by flow cytometric measurement of streptavidinFITC binding and is given as percentage of maximal binding determined in vitro. (C) Detection of sGPVI/JAQ1biotin complex in plasma at the indicated time points after antibody injection. (A-C) Results are expressed as the mean platelet count ± SD for groups of 6 mice each. (D) Western blot analysis of platelets at the indicated time points. The results are representative of 3 individual experiments. Tamer Rabie et al. Blood 2007;110:529-535 ©2007 by American Society of Hematology

LAT is required for shedding but not for internalization/intracellular clearing of GPVI. Wild-type (•) or LAT−/− (○) mice received 20 μg biotinylated JAQ1 intravenously and (A) platelet counts were determined. LAT is required for shedding but not for internalization/intracellular clearing of GPVI. Wild-type (•) or LAT−/− (○) mice received 20 μg biotinylated JAQ1 intravenously and (A) platelet counts were determined. (B) Surface-bound JAQ1biotin was detected by flow cytometric measurement of streptavidinFITC binding and is given as percentage of maximal binding determined in vitro. (C) Detection of sGPVI/JAQ1biotin complex in plasma at the indicated time points after antibody injection. (A-C) Results are expressed as the mean platelet count ± SD for groups of 6 mice each. (D) Western blot analysis of platelets at the indicated time points. The results are representative of 3 individual experiments. Tamer Rabie et al. Blood 2007;110:529-535 ©2007 by American Society of Hematology

LAT−/− mice are protected from JAQ1-induced bleeding. LAT−/− mice are protected from JAQ1-induced bleeding. (A) LAT−/− mice received 100 μg JAQ1 or JAQ3 intraperitoneally, and platelets were isolated on day 5 and tested in Western blot analysis for the presence of GPIIIa (EDL1) or GPVI (JAQ1, JAQ2, JAQ3). (B) Wild-type or LAT−/− mice received vehicle or 20 μg JAQ1 intravenously and platelets were isolated on day 5 followed by aggregometric analysis of convulxin (cvx)–induced aggregation. The arrows indicated the addition of the agonist (5 μg/mL). The results are representative of 6 individual experiments. (C) JAQ1 does not alter thrombin responses in LAT−/− mice. Washed platelets were prepared at the indicated time points after JAQ1 treatment and stimulated with 0.1 (▾), 0.01 (○), or 0.001 (•) U/mL thrombin. Activation of integrin αIIbβ3 and surface expression of P-selectin were determined by flow cytometry and are given as percentage ± SD of the values obtained with untreated controls (n = 6 per group). Platelets were gated by FSC/SSC characteristics and FL-4 positivity (anti–GPIbα-Cy5). (D) Tail bleeding times in wild-type and LAT−/− mice 24 hours after treatment with vehicle or 20 μg JAQ1. Tamer Rabie et al. Blood 2007;110:529-535 ©2007 by American Society of Hematology

A PLCγ2-independent pathway of GPVI-down-regulation. A PLCγ2-independent pathway of GPVI-down-regulation. Wild-type (•) or PLCγ2−/− (○) mice received 20 μg biotinylated JAQ1 intravenously and (A) platelet counts were determined. (B) Surface-bound JAQ1biotin was detected by flow cytometric measurement of streptavidinFITC binding and is given as percentage of maximal binding determined in vitro. (C) Detection of sGPVI/JAQ1biotin complex in plasma at the indicated time points after antibody injection. (A-C) Results are expressed as the mean platelet count ± SD for groups of 6 mice each. (D) Western blot analysis of platelets at the indicated time points. The results are representative of 3 individual experiments. (E) JAQ1 does not alter thrombin responses in PLCγ2−/− mice. Washed platelets were prepared at the indicated time points after JAQ1 treatment and stimulated with 0.1 (▾), 0.01 (○), or 0.001 (•) U/mL thrombin. Activation of integrin αIIbβ3 and surface expression of P-selectin were determined by flow cytometry and are given as percentage ± SD of the values obtained with untreated controls (n = 6 per group). Platelets were gated by FSC/SSC characteristics and FL-4 positivity (anti–GPIbα-Cy5). Tamer Rabie et al. Blood 2007;110:529-535 ©2007 by American Society of Hematology