Morphologic Assessment of Extracellular Matrix Scaffolds for Patch Tracheoplasty in a Canine Model Thomas W. Gilbert, PhD, Sebastien Gilbert, MD, Matthew.

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Morphologic Assessment of Extracellular Matrix Scaffolds for Patch Tracheoplasty in a Canine Model Thomas W. Gilbert, PhD, Sebastien Gilbert, MD, Matthew Madden, BS, Susan D. Reynolds, PhD, Stephen F. Badylak, DVM, MD The Annals of Thoracic Surgery Volume 86, Issue 3, Pages 967-974 (September 2008) DOI: 10.1016/j.athoracsur.2008.04.071 Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 (A) Photograph of surgical placement of decellularized tracheal matrix (left) for patch tracheoplasty. (B) Representative bronchoscopic image of remodeled extracellular matrix (ECM) at 6 months after surgical placement for patch tracheoplasty. The remodeled area is the whitish area in on the top surface of the trachea. (C) Representative gross morphologic image of remodeled ECM at 6 months after surgical placement for patch tracheoplasty. The remodeled area (arrow) consists of dense collagenous tissue covered with a shiny epithelial layer. There is no evidence of cartilage formation in the remodeled area. The Annals of Thoracic Surgery 2008 86, 967-974DOI: (10.1016/j.athoracsur.2008.04.071) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 (A) Histologic image of remodeled urinary bladder matrix (UBM) stained with periodic acid Schiff shows columnar ciliated epithelium with scattered secretory cells (original magnification ×200). (B) Average score of ciliated cells as a function of longitudinal distance for all remodeled UBM specimens (2 months, diamonds; 6 months. squares). (C) Average secretory cell count as a function of longitudinal distance from proximal to distal edge for the UBM specimens. (D) Average basal cell count as a function of longitudinal distance from proximal to distal edge for the UBM specimens. The Annals of Thoracic Surgery 2008 86, 967-974DOI: (10.1016/j.athoracsur.2008.04.071) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 (A) Histologic image of remodeled urinary bladder matrix crosslinked with carbodiimide (UBMC) stained with periodic acid Schiff shows columnar ciliated epithelium with scattered secretory cells (original magnification ×200). (B) Average score of ciliated cells as a function of longitudinal distance for all remodeled UBMC specimens (2 months, diamonds; 6 months, squares). (C) Average secretory cell count as a function of longitudinal distance from proximal to distal edge for the UBMC specimens. (D) Average basal cell count as a function of longitudinal distance from proximal to distal edge for the UBMC specimens. The Annals of Thoracic Surgery 2008 86, 967-974DOI: (10.1016/j.athoracsur.2008.04.071) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 (A) Histologic image of remodeled decellularized tracheal matrix (DTM) stained with periodic acid Schiff shows columnar ciliated epithelium with scattered secretory cells (original magnification ×200). (B) Average score of ciliated cells as a function of longitudinal distance for all remodeled DTM specimens. (C) Average secretory cell count as a function of longitudinal distance from proximal to distal edge for the DTM specimens. (D) Average basal cell count as a function of longitudinal distance from proximal to distal edge for the DTM specimens. The Annals of Thoracic Surgery 2008 86, 967-974DOI: (10.1016/j.athoracsur.2008.04.071) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions