Trimeric association of TMDs of Ebola virus GP, SARS CoV S, rabies virus GP, and influenza virus HA, as determined by SE-AUC. Trimeric association of TMDs.

Slides:

Advertisements

Similar presentations

Anna K. Wright, Ivan Hung, D. D. Busath, A. Kolocouris, Timothy A

Advertisements

Phylogenetic characterization of the Bunyavirales-like viruses identified in this study. Phylogenetic characterization of the Bunyavirales-like viruses.

Virulence of aggregate-forming and nonaggregate strains of Candida auris compared to C. albicans in Galleria mellonella larvae at 30°C (upper panel) and.

Volume 363, Issue 9413, Pages (March 2004)

by Sunghyouk Park, Michael E. Johnson, and Leslie W.-M. Fung

Volume 363, Issue 9413, Pages (March 2004)

Comparison of the model incorporating daily dose to that incorporating Cmax. Comparison of the model incorporating daily dose to that incorporating Cmax.

Comparison of AFP variants in HPLC assay, NMR spectroscopy, and bioactivity assay. Comparison of AFP variants in HPLC assay, NMR spectroscopy, and bioactivity.

Exploration of the data set with age as a continuous variable.

Volume 23, Issue 11, Pages (November 2015)

Acid stability of the HA protein of clade

ClustalOmega alignment of FlaC protein sequences.

Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae phages. Two parts of the proteomic tree with EVGs of putative Flavobacteriaceae.

Cryo-EM image reconstruction of the 2D3/MNV complex fitted with the atomic models of the MNV P domain dimer and two of the MAb 2D3 Fabs. Cryo-EM image.

H3 HA amino acid mutations that were selected in indicated clades.

Structure of the Angiopoietin-2 Receptor Binding Domain and Identification of Surfaces Involved in Tie2 Recognition William A. Barton, Dorothea Tzvetkova,

MFS1 expression in Z. tritici field isolates with different promoter genotypes. MFS1 expression in Z. tritici field isolates with different promoter genotypes.

Effects of length-altering chemical treatments are more apparent after F-L synchronization. Effects of length-altering chemical treatments are more apparent.

Fig. 6. Epitope mapping of C12G6.

DNSP16 mutation attenuates the virulence of a mouse-adapted MERS-CoV strain. dNSP16 mutation attenuates the virulence of a mouse-adapted MERS-CoV strain.

Volume 26, Issue 2, Pages e3 (February 2018)

Static Light Scattering From Concentrated Protein Solutions II: Experimental Test of Theory for Protein Mixtures and Weakly Self-Associating Proteins

Volume 85, Issue 4, Pages (October 2003)

Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,

Predicted microbiota age against the actual age of visitors and villagers. Predicted microbiota age against the actual age of visitors and villagers. (A)

GII.4E MAb binding is regulated by global particle conformation and surrounding local topology. GII.4E MAb binding is regulated by global particle conformation.

Differential relative abundance of major taxa for successive pairwise comparisons. Differential relative abundance of major taxa for successive pairwise.

Core Structure of gp41 from the HIV Envelope Glycoprotein

Parameters for SE-AUC. Parameters for SE-AUC. (A) Chimeric proteins were designed for SE-AUC, in which the TMD of interest was fused with the protein staphylococcal.

Inhibitory effect of T-705, ribavirin, T-1105, and T-1106 on SFTSV replication in Vero cells. Inhibitory effect of T-705, ribavirin, T-1105, and T-1106.

Histidine kinases are expanded in the fusaria.

Implicit adaptation generalizes around the aiming location (30° CCW)

αβ T Cell Receptor Ligand-Specific Oligomerization Revisited

Thermal and pH stability of influenza viruses.

Phylogenetic tree of K. variicola and K

Fig. 6. CXM correlates with age and growth velocity.

Summary curves of viral shedding in experimental influenza virus infection, according to the virus type or subtype. Summary curves of viral shedding in.

S. pneumoniae GapA is sensitive to oxygen and H2O2 exposure.

ZIKV ELISA results by NHP type and prior exposure.

Introduction of an autocatalytic hammerhead ribozyme sequence permits use of the optimal T7 promoter to enhance transcription levels. Introduction of an.

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

NP sequence alignment of human H1N1 strains and phylogenetic analysis of representative NP from pandemic and seasonal H1N1 strains. NP sequence alignment.

Antibody reactivity against HA1 proteins after vaccination.

Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp for each of 13 serotypes. Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp.

(A) Taxonomic identity at the phylum level of raw leachate (RL) and enrichment microcosms (E) as determined via Ion Torrent 16S rRNA gene amplicon sequencing.

Relative abundances of microeukaryotic taxa detected by ITS2 analysis of DNA or cDNA from stool. Relative abundances of microeukaryotic taxa detected by.

Functional distribution of genes activated upon exposure to C

Change in fluorescence of C. neoformans upon addition of DM262.

Characterization of chuvirus-like viruses.

Gallium isotopes at m/z and 70

Impact of medical data on bacterial diversity of fractionated samples.

Phylogenetic tree of K. variicola from humans and animals.

Distribution of signaling molecules in a section of P

KEGG cluster of DEGs of S

Comparison of community fungal and bacterial/archaeal diversity levels

Binocular neurons mismatched in spatial frequency are also mismatched in orientation preference. Binocular neurons mismatched in spatial frequency are.

Dynamic contrast-enhancement curves for patients with SLE (blue) and healthy controls (red) according to the brain regions sampled. Dynamic contrast-enhancement.

by John Misasi, Morgan S. A

Shannon rarefaction analysis.

Unsupervised principal-component analysis (PCA) of 129 seroreactive antigens for adults and children from different geographical locations. Unsupervised.

ROC analysis of MIC-1 and CA19-9.

PHs of olive brines. pHs of olive brines. The average ± standard deviation (SD) of two independent replicates at each time point is shown. Acetic acid.

Distribution of selected traits across the >4,000 strains in the most recent version of the database, including cell shape (A), spore formation (B), motility.

Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock communities. Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock.

Primary screening results of the MMV Malaria Box library.

DNSP16 mutant MERS-CoV infection produces minimal changes in early host responses. dNSP16 mutant MERS-CoV infection produces minimal changes in early host.

LFO1 degrades heme to a distinct catabolite.

PfHsp70x knockout does not inhibit export of PfEMP1 to the host cell.

αβ T Cell Receptor Ligand-Specific Oligomerization Revisited

Residues 327 and 404 are key binding sites for GII. 4F MAb

Presentation transcript:

Trimeric association of TMDs of Ebola virus GP, SARS CoV S, rabies virus GP, and influenza virus HA, as determined by SE-AUC. Trimeric association of TMDs of Ebola virus GP, SARS CoV S, rabies virus GP, and influenza virus HA, as determined by SE-AUC. Representative absorbance spectra for each SN-TMD are shown from analysis at 20,000 rpm. Absorbance data are plotted against normalized radial position and analyzed to determine best fit using χ2 and R values and residual plotting. Analysis is shown for the monomer-trimer (1:3) (blue) and monomer-trimer-hexamer (1:3:6) (red) curve fits. Best fit changed from pH 7 to 5 for influenza virus HA, so both sets of data are shown. Residuals are plotted above the absorbance spectra. The residuals for the monomeric fit are also shown in black. The monomeric curve fit is indicated by the black curve on each absorbance data plot. The residuals for the 1:3 (blue) and 1:3:6 (red) fits are overlaid to demonstrate the most appropriate fit. For example, the Ebola virus GP data points fit both 1:3 and 1:3:6 models according to the χ2 and R values; however, the residual plotting makes it apparent that the 1:3:6 fit is the most appropriate. Stacy R. Webb et al. mSphere 2018; doi:10.1128/mSphere.00047-18