Evelyn C. Nieves, Tomomi Toubai, Daniel C

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STAT3 Expression in Host Myeloid Cells Controls Graft-versus-Host Disease Severity Evelyn C. Nieves, Tomomi Toubai, Daniel C. Peltier, Katherine Oravecz-Wilson, Chen Liu, Hiroya Tamaki, Yaping Sun, Pavan Reddy Biology of Blood and Marrow Transplantation Volume 23, Issue 10, Pages 1622-1630 (October 2017) DOI: 10.1016/j.bbmt.2017.06.018 Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 LysM-Cre/STAT3fl/− mice showed reduced expression of STAT3 in MFs. (A) Western blot showing total STAT3 (top) and β-actin (middle) for B6 MFs, LysM-Cre/STAT3fl/− MFs, B6-WT DCs, and LysM-Cre/STAT3fl/− DCs. (Bottom) Relative increase in RNA expression of STAT3 in both B6-WT and LysM-Cre/STAT3fl/− MFs by qRT-PCR. (B) Representative dot plots and gates of peritoneal CD11b+F4/80+ cells from B6-WT and LysM-Cre/STAT3fl/− mice. (C) Representative expression levels of MHC class II (I-Ab), CD40, CD80, PD-L1, PD-L2, Gr-1, and B220 on CD11b+F4/80+ peritoneal MFs from B6-WT and LysM-Cre/STAT3fl/− mice. Error bars show mean ± SEM. Biology of Blood and Marrow Transplantation 2017 23, 1622-1630DOI: (10.1016/j.bbmt.2017.06.018) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Reduced STAT3 expression in host myeloid APCs amplifies the severity of acute GVHD. Host B6-WT and B6-LysM/Cre STAT3fl/− mice were lethally irradiated (11 Gy) on day -1 and infused with 2 × 106 CD90.2+ T cells along with 5 × 106 BM cells from either syngeneic B6 or allogeneic BALB/c mice on day 0. (A) Survival (n = 12-16 per group). Data are combined from three experiments with similar results. (B) GVHD clinical score. (C) Representative hematoxylin and eosin-stained images of liver on day 14 after allo-BMT. (D and E) Histopathological GVHD scores from liver (D) and GI tract (E) on day +14 after allo-BMT (n = 12 per group, pooled from two experiments). (F and G) Balb/c-WT mice were irradiated (8 Gy) and then immediately injected i.p. with 20 mg/kg WP1066 STAT3 inhibitor or diluent. At 24 and 48 hours after injection, the mice were euthanized, and whole spleens (F) and peritoneal (G) macrophages were harvested (n = 3 per group). Cells were analyzed for CD11b+ cells within the myeloid gate. Error bars show mean ± SEM. **P < .01; *P < .05. Biology of Blood and Marrow Transplantation 2017 23, 1622-1630DOI: (10.1016/j.bbmt.2017.06.018) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Deficiency of STAT3 in host MFs enhances donor T cell expansion after allogeneic BMT. Host B6-WT and B6-LysM/Cre STAT3fl/− mice were lethally irradiated (11 Gy) on day -1 and infused with 2 × 106 CD90.2+ T cells along with 5 × 106 BM cells from either syngeneic B6 or allogeneic BALB/c mice on day 0. (A) To evaluate donor T cell (H-2kd+CD4+ or H-2kd+CD8+) expansion, spleen cells from B6-WT and B6 LysM/Cre STAT3fl/− mice were harvested on day +14 after allogeneic BMT, stained, and analyzed by flow cytometry (n = 6 per group, pooled from two experiments). (B and C) Serum was collected from recipients on day +14, and IFN-γ (B) and IL-17A (C) levels were determined by ELISA (n = 6 per group, pooled from two experiments). (D and E) Donor CD4+Foxp3+ Treg expansion (D) and the ratio of Teff cells (CD4+FoxP3− and CD8+FoxP3−) to Treg cells (E) on day +14 after allo-BMT. Error bars show mean ± SEM. *P < .05. Biology of Blood and Marrow Transplantation 2017 23, 1622-1630DOI: (10.1016/j.bbmt.2017.06.018) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 4 STAT3-deficient macrophages show enhanced stimulation of allogeneic T cells in vitro. (A) Peritoneal MFs from B6-WT and LysM-Cre STAT3fl/− mice were used as stimulators in an MLR with T cells from either syngeneic B6 or allogeneic BALB/c mice and analyzed for T cell proliferation via 3H-thymidine incorporation at 72 hours. (B-F) Supernatants from MLR cultures were collected at 72 hours and analyzed by ELISA for IL-2 (B), IFN-γ (C), IL-17A (D), IL-10 (E), and IL-4 (F). The data are representative of three independent experiments. Error bars show mean ± SEM. * P < .05. Biology of Blood and Marrow Transplantation 2017 23, 1622-1630DOI: (10.1016/j.bbmt.2017.06.018) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 5 STAT3 deficiency in donor myeloid cells is dispensable for acute GVHD severity. Syngeneic controls were irradiated (8 Gy) on day -1 and infused with 5 × 106 TCD-BM cells along with 1 × 106 CD90.2+ T cells from B6- LysM-Cre/STAT3fl/− mice on day 0 (■, n = 12). Allogeneic host BALB/c mice were irradiated as above and infused with B6-WT 2 × 106 CD90.2+ T cells along with 5 × 106 TCD-BM from either WT-B6 (●, n = 12) or B6-LysM/Cre STAT3fl/− mice (▲, n = 12). The recipients were monitored for survival (A) and GVHD clinical scores (B) on a weekly basis. Data are combined from three experiments with similar results. (C-F) Serum was collected from recipients on days +7 and +14 after allo-BMT, and cytokine levels were determined by ELISA for IFN-γ (C), TNF-α (D), IL-17A (E), and IL-1β (F). *P < .05. (G-I) Spleen cells from B6-WT and B6 LysM/Cre STAT3fl/− mice were harvested on day +7 after allo-BMT, stained, and analyzed by flow cytometry. Donor T cell [H-2 kb+CD4+ (G) and H-2 kb+CD8+ (H)] expansion, donor CD4+Foxp3+ Treg cell expansion (I), and the ratio of Teff cells (CD4+FoxP3− and CD8+FoxP3−) to Treg cells (J) on day 7 after allo-BMT are shown (n = 4 per group, pooled from two experiments). Error bars show mean ± SEM. Biology of Blood and Marrow Transplantation 2017 23, 1622-1630DOI: (10.1016/j.bbmt.2017.06.018) Copyright © 2017 The American Society for Blood and Marrow Transplantation Terms and Conditions