Figure 2 Expression of GABAA receptor and LGI1 by patient's thymomaTissue sections of the patient's thymoma incubated with biotinylated immunoglobulin.

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Figure 2 Expression of GABAA receptor and LGI1 by patient's thymomaTissue sections of the patient's thymoma incubated with biotinylated immunoglobulin G (IgG) from a patient with only GABAA receptor antibodies (A), another patient with only LGI1 antibodies (C), and a control subject without antibodies (E) compared with the reactivity of a commercial antibody against GABAA receptor (B) (rabbit polyclonal anti-α1 GABAA receptor, abcam, ab33299, diluted 1:50), a commercial antibody against LGI1 (D) (rabbit polyclonal anti-LGI1, abcam, ab30868, diluted 1:50), and the secondary antibody alone (F). Expression of GABAA receptor and LGI1 by patient's thymomaTissue sections of the patient's thymoma incubated with biotinylated immunoglobulin G (IgG) from a patient with only GABAA receptor antibodies (A), another patient with only LGI1 antibodies (C), and a control subject without antibodies (E) compared with the reactivity of a commercial antibody against GABAA receptor (B) (rabbit polyclonal anti-α1 GABAA receptor, abcam, ab33299, diluted 1:50), a commercial antibody against LGI1 (D) (rabbit polyclonal anti-LGI1, abcam, ab30868, diluted 1:50), and the secondary antibody alone (F). Note the similar pattern of reactivity of human antibodies and commercial antibodies, indicating that the tumor expresses epitopes of GABAA receptor and LGI1 proteins. The dilution of human IgG was 1:50. All sections were mildly counterstained with hematoxylin. Mateus Mistieri Simabukuro et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e73 © 2015 American Academy of Neurology