CLASSIFICATION OF ANEMIAS

LEARNING OBJECTIVE CLASSIFY ANAEMIAS

Chronic: GIT lesions,gynaecological disturbances 1. BLOOD LOSS Acute: trauma Chronic: GIT lesions,gynaecological disturbances 2. INCREASED RATE OF RED CELL DESTRUCTION INTRINSIC ABNORMALITIES OF RED CELLS Red cell membrane disorders Hereditary spherocytosis Red cell enzyme deficiences G6PD deficiency

Disorders of haemoglobin synthesis Quantitative defect of globin synthesis- Thalassemias Qualitative defect of globin synthesis - Sickle cell anaemia EXTRINSIC ABNORMALITIES OF RED CELLS Antibody mediated transfusion reactions Idiopathic Drug induced

Mechanical trauma to red cells Microangiopathic hemolytic anaemia Thrombotic thrombocytopenia purpura DIC Severe vasculitis CHEMICAL INJURY Lead poisoning

3.IMPAIRED RED CELL PRODUCTION DISTURBANCE OF PROLIFERATION OF RED CELLS Aplastic anaemia DISTURBANCE OF MATURATION OF ERYTHROBLAST Defective DNA Synthesis -Vitamin B12 and folate Defective hemoglobin synthesis Deficient heme synthesis- Iron deficiency anaemia Deficient globin synthesis - Thalassemias

MATERIALS AND METHODS 50 patients of ALL SAMPLE SIZE 50 patients of ALL SAMPLING TECHNIQUE Non probability purposive sampling

SPECIMEN COLLECTION Three ml venous blood in EDTA Bone marrow aspirate in EDTA Bone marrow aspiration smears Genomic DNA was extracted from blood/bone marrow

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