Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation Pascal Jeanmonod, MD, Matthias.

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Presentation transcript:

Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation Pascal Jeanmonod, MD, Matthias W. Laschke, PhD, Nils Gola, Maximilian von Heesen, MD, Matthias Glanemann, MD, Stefan Dold, MD, Michael D. Menger, MD, Mohammed R. Moussavian, MD Journal of Vascular Surgery Volume 58, Issue 6, Pages 1637-1643 (December 2013) DOI: 10.1016/j.jvs.2013.02.012 Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 1 A, Stereomicroscopic overview of a Dacron Silver vascular graft (2 × 2 × 0.35 mm) directly after implantation onto the striated muscle tissue in the dorsal skinfold chamber of a C57BL/6 mouse. Note that the larger microvessels of the chamber (arterioles and venules of the striated muscle and subcutaneous tissue) can be identified without the use of high-resolution intravital fluorescence microscopy. B, The density of newly formed blood-perfused microvessels growing into the implant was measured in eight different microvascular regions-of-interest (ROIs; red boxes; size: 0.4 mm²), which were directly located at the interface between the prosthetic vascular graft and the surrounding host tissue. In addition, four different ROIs in the border zone of the implant, (ie, in close vicinity to the material [blue boxes]), leukocyte-endothelial cell interaction, and microhemodynamics were measured in postcapillary and collecting venules of the host tissue. Journal of Vascular Surgery 2013 58, 1637-1643DOI: (10.1016/j.jvs.2013.02.012) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 2 Intravital fluorescent microscopic images of the border of Dacron (A) and Dacron Silver vascular grafts (B) at day 14 after implantation into the dorsal skinfold chamber of C57BL/6 mice. Blue light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate (FITC)-labeled dextran 150,000 i.v. C, Functional capillary density (cm/cm²) within the border of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8), as assessed by intravital fluorescence microscopy and computer-assisted image analysis. Means ± standard error of the mean (SEM). *P < .05 vs Dacron at corresponding time points. Journal of Vascular Surgery 2013 58, 1637-1643DOI: (10.1016/j.jvs.2013.02.012) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 3 Rolling leukocytes (A, min−1) and adherent leukocytes (B, mm−2) in postcapillary and collecting venules within the border zones of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8), as assessed by intravital fluorescence microscopy and computer-assisted image analysis. Means ± standard error of the mean (SEM). *P < .05 vs Dacron at corresponding time points. Journal of Vascular Surgery 2013 58, 1637-1643DOI: (10.1016/j.jvs.2013.02.012) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 4 Hematoxylin-eosin stained cross sections of the border zone of a Dacron Silver vascular graft (asterisk) at day 14 after implantation onto the striated muscle tissue (A, arrows) within the dorsal skinfold chamber of a C57BL/6 mouse. Higher magnification (B) of the interface between the implant and the surrounding tissue (A, black window) shows the dense vascularization and ingrowth of a fibrovascular tissue in between the Dacron fibers. Journal of Vascular Surgery 2013 58, 1637-1643DOI: (10.1016/j.jvs.2013.02.012) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 5 Histologic cross-sections of the border zones of a Dacron (A, C, E) and a Dacron Silver vascular graft (B, D, F) at day 14 after implantation onto the striated muscle tissue within the dorsal skinfold chambers of C57BL/6 mice. The sections were stained with Sirius red (A and B) for the visualization of collagen fibers or antibodies against proliferating cell nuclear antigen (PCNA) (C and D) and caspase-3 (casp-3) (E and F) for the detection of proliferating and apoptotic cells. PCNA-positive cells (G, %) and casp-3-positive cells (H, %) in the border zones of Dacron (white bars; n = 8) and Dacron Silver vascular grafts (black bars; n = 8) at day 14. Means ± standard error of the mean (SEM). Journal of Vascular Surgery 2013 58, 1637-1643DOI: (10.1016/j.jvs.2013.02.012) Copyright © 2013 Society for Vascular Surgery Terms and Conditions