Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model Ryan O. Lakin, MD, Weifei Zhu, PhD, Lindsay.

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Presentation transcript:

Techniques to harvest diseased human peripheral arteries and measure endothelial function in an ex vivo model Ryan O. Lakin, MD, Weifei Zhu, PhD, Lindsay Feiten, BS, Vikram S. Kashyap, MD Journal of Vascular Surgery Volume 58, Issue 2, Pages 470-477 (August 2013) DOI: 10.1016/j.jvs.2012.10.123 Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 1 The endothelial-dependent relaxation (EDR) curve was established using incremental doses of acetylcholine (Ach). NE, Norepinephrine. Journal of Vascular Surgery 2013 58, 470-477DOI: (10.1016/j.jvs.2012.10.123) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 2 Intravascular ultrasound is able to predict the composition of plaques without any a priori information on the histology of the vessel. A, An anterior tibial artery is seen in this gray-scale intravascular ultrasound image. B, A virtual histology image is created using a modified coronary artery algorithm classified by color indicating calcium (white), necrosis (red), and fibrous (green) plaque morphology. Journal of Vascular Surgery 2013 58, 470-477DOI: (10.1016/j.jvs.2012.10.123) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 3 Effect of l-arginine supplementation is shown on human lower extremity arteries. Endothelial-dependent relaxation (EDR) is augmented in the l-arginine group (P < .0001) by two-way analysis of variance. N-nitro-l-arginine methyl ester (L-NAME) blocked acetylcholine (Ach)-induced EDR (P < .0001). Values are shown ± standard error of the mean. *P < .05, **P < .01 with Bonferroni post-test comparisons for l-arginine vs l-NAME groups. Journal of Vascular Surgery 2013 58, 470-477DOI: (10.1016/j.jvs.2012.10.123) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 4 Immunohistochemistry staining of (A) cluster of differentiation (CD) 31, (B) endothelial nitric oxide synthase (eNOS), and (C) arginase II demonstrates intact arterial endothelium (original magnification, ×10). Journal of Vascular Surgery 2013 58, 470-477DOI: (10.1016/j.jvs.2012.10.123) Copyright © 2013 Society for Vascular Surgery Terms and Conditions

Fig 5 Scatter plot analysis displays correlation of endothelial-dependent relaxation (EDR) to total plaque volume, percentage of plaque cross-sectional area (PCSA), average percentage occlusion (Occ), average percentage necrotic core (NC), and endothelial-independent relaxation (EIR). R, R2, and P values are shown for each plot. CSA, Cross-sectional area. Journal of Vascular Surgery 2013 58, 470-477DOI: (10.1016/j.jvs.2012.10.123) Copyright © 2013 Society for Vascular Surgery Terms and Conditions