Salmonella enterica serovar typhimurium (S typhimurium) TML interacts at discrete foci with the brush border of human terminal ileal epithelium. Salmonella.

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Salmonella enterica serovar typhimurium (S typhimurium) TML interacts at discrete foci with the brush border of human terminal ileal epithelium. Salmonella enterica serovar typhimurium (S typhimurium) TML interacts at discrete foci with the brush border of human terminal ileal epithelium. Human terminal ileal epithelial tissue samples from 11 patients were incubated by in vitro organ culture (IVOC), either as uninfected controls (A) or with S typhimurium TML for four hours (five cases) (B), six hours (three cases) (C), or eight hours (three cases) (D). (B) Tissue sections were stained with haematoxylin-eosin and then analysed by light microscopy to search for areas along the apical surface of the epithelial cell monolayer where bacteria appeared closely associated with the tissue. Such areas were termed “foci of interaction” (F) (C, D). (E) Data depict the mean (SD) number of “foci of interaction” observed on 4 μm thick villus sections. A Haque et al. Gut 2004;53:1424-1430 Copyright © BMJ Publishing Group Ltd & British Society of Gastroenterology. All rights reserved.