In vitro effects of non-steroidal anti-inflammatory drugs on cytokine, prostanoid and matrix metalloproteinase production by interface membranes from.

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Presentation transcript:

In vitro effects of non-steroidal anti-inflammatory drugs on cytokine, prostanoid and matrix metalloproteinase production by interface membranes from loose hip or knee endoprostheses S.A. Syggelos, M.D., Ph.D., E. Giannopoulou, Ph.D., P.A. Gouvousis, Ph.D., A.P. Andonopoulos, M.D., Ph.D., F.A.C.P., A.J. Aletras, Ph.D., E. Panagiotopoulos, M.D., Ph.D. Osteoarthritis and Cartilage Volume 15, Issue 5, Pages 531-542 (May 2007) DOI: 10.1016/j.joca.2006.11.003 Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Representative western blotting of an IFT culture conditioned medium, using polyclonal antibodies against human MMP-1 (A), MMP-3 (B), MMP-13 (C) and TIMP-2 (D). Markers of known molecular mass are shown to the right. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Levels of IL-1β (A), IL-6 (B) and TNF-α (C) in conditioned media obtained from 3-day explant cultures of IFT from hip (circle symbols) or knee (square symbols) arthroplasty, in the absence or presence of drugs. Closed symbols represent a statistically significant difference between NSAID exposed and unexposed cultures (P<0.05). Ace1 and 2: aceclofenac at concentrations 4 and 10μg/ml, respectively; Pir1 and 2: piroxicam at concentrations 3 and 10μg/ml, respectively; Ten1 and 2: tenoxicam at concentrations 6 and 13μg/ml, respectively; Ind: indomethacin at concentration 0.5μg/ml and Par: paracetamol at concentration 10μg/ml. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Representative gelatin zymographies of conditioned media obtained from 3-day explant culture of an IFT from hip arthroplasty, in the absence or presence of NSAIDs. (A) Aceclofenac at concentrations 4 (lanes a–c) and 10 (lanes d–f) μg/ml, (B) piroxicam at concentrations 3 (lanes a–c) and 10 (lanes d–f) μg/ml, (C) tenoxicam at concentrations 6 (lanes a–c) and 13 (lanes d–f) μg/ml, (D) indomethacin at concentration 0.5μg/ml (lanes a–c) and paracetamol at concentration 10μg/ml (lanes d–f), and (E) controls without drugs. The conditioned media from three independent experiments with each drug concentration were run in duplicate. proMMP-9, proenzyme of MMP-9; act MMP-9, partially activated form of MMP-9; proMMP-2, proenzyme of MMP-2; act MMP-2, activated form of MMP-2. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Metalloproteinase MMP-2 (A) and MMP-9 (B) activity in conditioned media obtained from 3-day explant cultures of IFT from hip (circle symbols) or knee (square symbols) arthroplasty, in the absence or presence of drugs. Closed symbols represent a statistically significant difference between NSAID exposed and unexposed cultures (P<0.05). The abbreviations for NSAIDs and their concentrations used are as in Fig. 2. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Levels of MMP-1 (A), MMP-3 (B) and MMP-13 (C) in conditioned media obtained from 3-day explant cultures of IFT from hip (circle symbols) or knee (square symbols) arthroplasty, in the absence or presence of drugs. Closed symbols represent a statistically significant difference between NSAID exposed and unexposed cultures (P<0.05). The abbreviations for NSAIDs and their concentrations used are as in Fig. 2. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Levels of TIMP-1 (A) and TIMP-2 (B) in conditioned media obtained from 3-day explant cultures of IFT from hip (circle symbols) or knee (square symbols) arthroplasty, in the absence or presence of drugs. Closed symbols represent a statistically significant difference between NSAID exposed and unexposed cultures (P<0.05). The abbreviations for NSAIDs and their concentrations used are as in Fig. 2. Osteoarthritis and Cartilage 2007 15, 531-542DOI: (10.1016/j.joca.2006.11.003) Copyright © 2006 Osteoarthritis Research Society International Terms and Conditions