A recent epidemiological cluster of acute hepatitis B genotype F1b infection in a restricted geographical area of Italy M. Pollicita, C. Alteri, M.C.

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A recent epidemiological cluster of acute hepatitis B genotype F1b infection in a restricted geographical area of Italy M. Pollicita, C. Alteri, M.C. Bellocchi, D. Armenia, L. Carioti, R. Salpini, L. Colagrossi, A. Battisti, M. Aragri, L. Fabeni, R. Mariani, M. Dalessandro, A. Ranelli, M. Paoloni, G. Parruti, C.F. Perno, V. Svicher Clinical Microbiology and Infection Volume 21, Issue 12, Pages 1124.e1-1124.e4 (December 2015) DOI: 10.1016/j.cmi.2015.07.022 Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 (a) A maximum-likelihood phylogenetic tree was constructed by using hepatitis B virus (HBV) F1b reverse transcriptase (RT) sequences from: 70 isolates downloaded from GenBank, eight individuals involved in the cluster, and two individuals collected from routine laboratory testing at the university hospital ‘Tor Vergata’ (identified with arabic numbers). Two HBV F1a RT sequences were used as the outgroup. (b) A maximum-likelihood phylogenetic tree was constructed by using concatenated HBV F1b basic core promoter (BCP)/precore/core/RT sequences from: 58 isolates downloaded from GenBank, and five individuals involved in the cluster. Two HBV F1a BCP/precore/core/RT sequences were used as the outgroup. Branch lengths were estimated with the best-fitting nucleotide substitution model (GTR + G + I) according to a hierarchical likelihood ratio test. The bar at the bottom indicates 0.001 nucleotide substitutions per site. The asterisks (*) along a branch represent bootstrap support of >85%. The clusters involving the eight HBV F1b RT sequences and the five concatenated RT/HBsAg/BCP/precore/core sequences are in the grey box. Clinical Microbiology and Infection 2015 21, 1124.e1-1124.e4DOI: (10.1016/j.cmi.2015.07.022) Copyright © 2015 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions