Improved Fecal DNA Test for Colorectal Cancer Screening Steven H. Itzkowitz, Lina Jandorf, Randall Brand, Linda Rabeneck, Paul C. Schroy, Stephen Sontag, David Johnson, Joel Skoletsky, Kris Durkee, Sanford Markowitz, Anthony Shuber  Clinical Gastroenterology and Hepatology  Volume 5, Issue 1, Pages 111-117 (January 2007) DOI: 10.1016/j.cgh.2006.10.006 Copyright © 2007 AGA Institute Terms and Conditions

Figure 1 Detection of vimentin methylation in fecal DNA analyzed by VIM-29 PCR primers. Amplification of fecal DNA from 9 normal (n) and 5 colon cancer (c) patients. Upper panel (VIM-29M) shows vimentin gene methylation and the lower panel (VIM-29U) shows control wild-type amplification of unmethylated vimentin sequences derived from normal cells in all samples. Positive (+) vimentin methylation is noted next to the sample identification number. Assay controls include unmethylated (u) and methylated (m) DNA samples, and negative water blank (deionized water). Clinical Gastroenterology and Hepatology 2007 5, 111-117DOI: (10.1016/j.cgh.2006.10.006) Copyright © 2007 AGA Institute Terms and Conditions