Firdaus Hamid, MD, PhD, Serge A. Versteeg, BSc, Aprilianto E

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Presentation transcript:

Molecular diagnostics and lack of clinical allergy in helminth-endemic areas in Indonesia Firdaus Hamid, MD, PhD, Serge A. Versteeg, BSc, Aprilianto E. Wiria, MD, PhD, Linda J. Wammes, MD, PhD, Sitti Wahyuni, MD, PhD, Taniawati Supali, PhD, Erliyani Sartono, PhD, Ronald van Ree, PhD, Maria Yazdanbakhsh, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 4, Pages 1196-1199.e6 (October 2017) DOI: 10.1016/j.jaci.2017.04.040 Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 IgE measured by ImmunoCAP and ImmunoCAP-ISAC. IgE to HDM (D pteronyssinus) (A), cockroach (B), peanut (C), grass pollen (D), and shrimp (E) measured by ImmunoCAP and ImmunoCAP-ISAC. The single allergens depicted for ISAC are related to the whole allergen extracts tested in CAP. Solid circles represent natural (n) component allergens, and open circles represent recombinant (r) component allergens. Horizontal dotted lines represent the cutoff value for ImmunoCAP positivity. IgE levels are given as KU/L for ImmunoCAP and as ISAC standardized units (ISU)/L for ImmunoCAP-ISAC. *For ImmunoCAP-ISAC assay, only positive results are shown. Journal of Allergy and Clinical Immunology 2017 140, 1196-1199.e6DOI: (10.1016/j.jaci.2017.04.040) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE to allergens. IgE to purified natural and recombinant allergens recognized on ImmunoCAP-ISAC (N = 150). Solid circles represent natural (n) component allergens, and open circles represent recombinant (r) component allergens. Cross-reacting allergen to *tropomyosin, #nonspecific lipid transfer protein (nsLTP), $the pathogenesis-related (PR)-10 protein, +CCDs. Each circle represents a single positive subject, and the dotted line indicates the cutoff value for the ISAC assay (0.3 ISU). ISU, ISAC standardized unit. The allergen abbreviations are given in Table E2. Indicated in red squares are allergens carrying CCD. Journal of Allergy and Clinical Immunology 2017 140, 1196-1199.e6DOI: (10.1016/j.jaci.2017.04.040) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 Correlation between IgE to CCD and natural allergens on ImmunoCAP-ISAC: walnut (A), bermuda (B), timothy (C), cypress (D), and plane tree (E). nMUXF3 is a marker glycoprotein for CCD reactivity on ISAC assay. ISU, ISAC standardized unit; r, correlation coefficient. Journal of Allergy and Clinical Immunology 2017 140, 1196-1199.e6DOI: (10.1016/j.jaci.2017.04.040) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 Correlation between IgE levels to bromelain and allergens on ImmunoCAP: timothy (A), peanut (B), cockroach (C), HDM (D pteronyssionus) (D), and shrimp (E). Bromelain is a marker glycoprotein for CCD reactivity. P values and r (correlation coefficient) are given. Journal of Allergy and Clinical Immunology 2017 140, 1196-1199.e6DOI: (10.1016/j.jaci.2017.04.040) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 IgE inhibition assay. IgE cross-reactivity of HDM (D pteronyssinus), cockroach, peanut, and grass pollen extract tested by using an IgE ImmunoCAP inhibition assay. Bromelain (A), soluble egg antigens (SEAs) of S haematobium (B), and A lumbricoides (C) were used as inhibitors and values were expressed as the percentage of IgE inhibition. Journal of Allergy and Clinical Immunology 2017 140, 1196-1199.e6DOI: (10.1016/j.jaci.2017.04.040) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions