Mucinous Differentiation Correlates with Absence of EGFR Mutation and Presence of KRAS Mutation in Lung Adenocarcinomas with Bronchioloalveolar Features

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Mucinous Differentiation Correlates with Absence of EGFR Mutation and Presence of KRAS Mutation in Lung Adenocarcinomas with Bronchioloalveolar Features Karin E. Finberg, Lecia V. Sequist, Victoria A. Joshi, Alona Muzikansky, Julie M. Miller, Moonjoo Han, Javad Beheshti, Lucian R. Chirieac, Eugene J. Mark, A. John Iafrate The Journal of Molecular Diagnostics Volume 9, Issue 3, Pages 320-326 (July 2007) DOI: 10.2353/jmoldx.2007.060182 Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Study organization including summary of histological features of 119 primary lung cancers submitted for EGFR mutation status evaluation and summary of morphological and molecular features of the BAC/AWBF subset of tumors. N.O.S., not otherwise specified. The Journal of Molecular Diagnostics 2007 9, 320-326DOI: (10.2353/jmoldx.2007.060182) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Morphological and molecular features of BAC/AWBF. A: Mucinous adenocarcinoma with prominent BAC component harboring KRAS mutation and lacking EGFR mutation. Inset, DNA sequence chromatogram displaying codons 12 and 13 of KRAS in the same tumor. A heterozygous G>A transition at nucleotide 35 of the KRAS coding sequencing is observed. B: Nonmucinous adenocarcinoma with prominent BAC component lacking EGFR mutation. C: Nonmucinous BAC with EGFR mutation. Inset, sequence tracing after codon 746 (GGA) consistent with L747_T751delinsP mutation (deletion confirmed by sequencing reverse strand; data not shown). D: EGFR gene copy number analysis by FISH demonstrating high polysomy in a mucinous BAC with KRAS mutation (35G>T) and lacking EGFR mutation. Red signal indicates probe hybridization to the chromosome 7p (EGFR locus), and green signal represents hybridization of the chromosome 7q reference probe. Original magnifications: ×200 (A–C); ×1000 (D). The Journal of Molecular Diagnostics 2007 9, 320-326DOI: (10.2353/jmoldx.2007.060182) Copyright © 2007 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions