NoRC affects centromere function.

Slides:



Advertisements
Similar presentations
Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.
Advertisements

Survivin is a determining factor for mitotic localization of the chromosome passenger complex. Survivin is a determining factor for mitotic localization.
Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted ES cells on LIF withdrawal. Myc‐induced miRNAs attenuate differentiation of c‐Myc‐depleted.
Reduced astrocyte proliferation results in increased immune cell infiltration into the injured GM Reduced astrocyte proliferation results in increased.
Reduced scar formation in the injured GM of CCR2−/− mice
Colocalization of the human trimethylguanosine synthase 1 and survival of motor neuron proteins. Colocalization of the human trimethylguanosine synthase.
Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.
PtdIns5P promotes cell migration.
SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.
Desmoplakin is a direct transcriptional target of Bcl11b.
Downregulation of ΔNp73 leads to re‐activation of p53‐regulated genes and apoptosis. Downregulation of ΔNp73 leads to re‐activation of p53‐regulated genes.
Loss of USP8 or HIF1α impairs endocytic recycling required for ciliogenesis Kinetic analysis of transferrin recycling measured by flow cytometry. Loss.
Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Dynamics of ookinete to oocyst transition, and effects of SRPN2 knockdown. Mosquitoes.
Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.
NM1 localizes in nucleoli and is required for Pol I transcription.
NoRC regulates telomeric heterochromatin.
Both NM1 and the WSTF complex stimulate Pol I transcription.
Jan-Philipp Mallm, Karsten Rippe  Cell Reports 
Increased astrocyte proliferation is paralleled with reduced infiltration of immune cells in mice with inducible SMOM2 expression in astrocytes at 5 dpi.
Remodeling of Three-Dimensional Organization of the Nucleus during Terminal Keratinocyte Differentiation in the Epidermis  Michal R. Gdula, Krzysztof.
Anaphase chromosome separation in extracts takes place in the absence of intact spindle poles. Anaphase chromosome separation in extracts takes place in.
Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.
Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.
Chromatin regulation upon recruitment of HOTAIR
Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.
FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL.
Reduced ALPI expression in patients’ small intestinal biopsies
SENP7 regulates chromatin decondensation required for HR repair.
Myc increases the instability of lac operator repeats.
Deficient segregation of the lac operator insertion.
Activation of DNA Damage Response Signaling by Condensed Chromatin
The psoriatic phenotype of DKO
Histone deacetylase 1 protein depletion affects general histone acetylation and specific gene expression. Histone deacetylase 1 protein depletion affects.
MOSPD2 silencing affects interorganelle contact sites organization
Human Centromeres Produce Chromosome-Specific and Array-Specific Alpha Satellite Transcripts that Are Complexed with CENP-A and CENP-C  Shannon M. McNulty,
Volume 37, Issue 5, Pages (June 2016)
Shane M. Harding, Jonathan A. Boiarsky, Roger A. Greenberg 
NDP52 promotes CHIKV infection in a species‐specific manner.
NoRC suppresses recombination between telomeric repeats.
MET inhibition promotes p21 nuclear translocation
Geoffrey J. Guimaraes, Yimin Dong, Bruce F. McEwen, Jennifer G. DeLuca 
Cbl protein levels in ago3, twin, and armi mutant GSCs
Pathogenic LRRK2 mutants co‐localize with Rab29 and disperse Golgi membranes Pathogenic LRRK2 mutants co‐localize with Rab29 and disperse Golgi membranes.
LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.
GRM7 Regulates Embryonic Neurogenesis via CREB and YAP
Volume 20, Issue 4, Pages (April 2011)
A kinase‐dead mutant and an ECO‐associated mutant of ICK show a decreased ability to rescue shortened cilia phenotype in ICK−/− MEFs A–FKinase activity.
Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs Assessment of proliferation status of PDGFRα+ cells in developing mouse lungs.
R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.
ZBTB48 is required for MTFP1 expression
T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells in tumor tissue T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells.
P2Y1 receptor mediated the directional migration of SVZ neuroblasts in an EF. (A) Suramin (general purinergic receptor inhibitor, 5 μM) did not affect.
GSC tumor phenotype in ago3 mutant germaria
Timing and Checkpoints in the Regulation of Mitotic Progression
Volume 13, Issue 3, Pages (February 2003)
HJURP is required for CENP-A localization to centromeres.
NLK positively regulates YAP activity in physiological settings
RECQL4 is a MAP with a spindle function.
Fig. 4. Perinuclear dynein regulators are not required for primary ciliogenesis.RPE cells were transfected with siRNA and either nocodazole-treated, fixed,
Intergenic Transcripts Regulate the Epigenetic State of rRNA Genes
Volume 25, Issue 11, Pages e5 (December 2018)
Fig. 6 Metarrestin treatment reduces pre-RNA synthesis and Pol I occupancy at rDNA without changing rDNA chromatin states. Metarrestin treatment reduces.
Engagement of PDGF-αR and PDGF-βR is critical for PDGF-BB-mediated increased proliferation of NPCs. RT-PCR (A) and WB (B) of PDGF-αR and PDGF-βR in NPCs;
Effect of M-cadherin RNAi on apoptosis in confluent C2C12 myoblasts.
Analysis of centromere and kinetochore function after depletion of DmSMC4. Analysis of centromere and kinetochore function after depletion of DmSMC4. (A)
Confocal images representing the localization of PCDH7
CROCC abrogates centrosome-related mitotic errors in 1p36
The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.
Volume 33, Issue 3, Pages (May 2015)
Fig. 5 hTERT promotion of Hsp70-1 telomere localization is dependent on Apollo and assists the telomere protective function of Apollo. hTERT promotion.
Presentation transcript:

NoRC affects centromere function. NoRC affects centromere function. (A) Depletion of TIP5 induces apoptosis. Percentage of apoptotic HeLa/Kyoto cells transfected with control siRNA (ctrl) or siRNA against TIP5 (siTIP5). Data represent mean values and error bars indicate s.e.m. (n=3). A representative apoptotic cell is shown in the inset. (B) Knockdown of TIP5 delays metaphase plate formation and prolongs mitosis. Representative images of mitotic HeLa/Kyoto cells 72 h after transfection with control (ctrl) and TIP5‐specific siRNA (siTIP5). The graph indicates the length of mitosis in a representative experiment (N=3), each dot represents one cell and red lines indicate the median duration of mitosis (Mann–Whitney test, *P=0.028, n=43). Scale bar, 5 μm. (C) NoRC is localized in nucleoli and the nucleoplasm. Three‐dimensional reconstruction of deconvolved image of an interphase U2OS cell nucleus immunostained with α‐TIP5 (red) and α‐UBF (green) antibodies. DNA was counterstained with DAPI. Scale bar, 5 μm. (D) TIP5 localizes at centromeres. Left: Z‐projection of a representative nucleus from U2OS cells immunostained with α‐TIP5 (red) and α‐CENPs antibodies (green). The same nucleus showing colocalization of TIP5 with CENPs (white spots) is shown below. Middle: magnification of an indicated section showing colocalization of TIP5 and CENPs (yellow). Scale bar, 5 μm. Right: quantification of TIP5‐positive spots colocalizating with CENPs in a representative experiment (N=4), each dot represents one cell (unpaired t‐test, P‐value ****<0.0001, n=45). (E) ChIP comparing TIP5 occupancy at major satellite repeats and the rDNA promoter in NIH3T3 cells. Error bars represent s.e.m. (n=3). (F) NoRC triggers heterochromatin formation at pericentromeres. H4K20me3 and H3K9me3 occupancy at pericentromeres of NIH3T3 cells overexpressing TIP5 (left) and in TIP5‐depleted cells (right). ChIPs from mock‐transfected cells were used as reference (ctrl=1). Error bars represent s.e.m. (n=3). ChIP, chromatin immunoprecipitation; DAPI, 4,6‐diamidino‐2‐phenylindole; NoRC, nucleolar remodelling complex; siRNA, short interfering RNA. Anna Postepska‐Igielska et al. EMBO Rep. 2013;14:704-710 © as stated in the article, figure or figure legend