K. Kawakita, T. Nishiyama, T. Fujishiro, S. Hayashi, N. Kanzaki, S

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B. Bai, Y. Li Osteoarthritis and Cartilage

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General Information Osteoarthritis and Cartilage

Presentation transcript:

Akt phosphorylation in human chondrocytes is regulated by p53R2 in response to mechanical stress K. Kawakita, T. Nishiyama, T. Fujishiro, S. Hayashi, N. Kanzaki, S. Hashimoto, K. Takebe, K. Iwasa, S. Sakata, K. Nishida, R. Kuroda, M. Kurosaka Osteoarthritis and Cartilage Volume 20, Issue 12, Pages 1603-1609 (December 2012) DOI: 10.1016/j.joca.2012.08.022 Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Immunohistochemistry for p53R2 in normal and osteoarthritic (OA) cartilage tissue. The dot plots graph show percentage of p53R2 positive cells at superficial and deep zone in normal and OA cartilage. (A) Expression of p53R2 in normal and OA chondrocytes, as detected by western blotting. (B) Expression of p53R2 in normal and OA chondrocytes, as examined by real-time PCR. (C) Each sample was derived from different individuals. Osteoarthritis and Cartilage 2012 20, 1603-1609DOI: (10.1016/j.joca.2012.08.022) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Expression of p53R2 in OA chondrocytes after applying various magnitudes (2%, 5%, and 10%) of tensile strain for 12 h was detected using western blotting (A) and real-time PCR (B). Each sample was derived from different individuals. Phosphorylation of p53 at Ser 15 and Ser 46 in OA chondrocytes was observed after applying 5% tensile strain (C). Osteoarthritis and Cartilage 2012 20, 1603-1609DOI: (10.1016/j.joca.2012.08.022) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 COL2A1 and aggrecan expression of OA chondrocytes after p53R2 siRNA and control siRNA transfection. (A) sGAG was quantified after transfection of the cells with p53R2 siRNA or control siRNA. (B) Each sample was derived from different individuals. Osteoarthritis and Cartilage 2012 20, 1603-1609DOI: (10.1016/j.joca.2012.08.022) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Phospho-Akt, Akt, phospho-p38MAPK, p38MAPK, phospho-ERK, ERK, phospho-JNK, JNK and p53R2 expression after applying 5% tensile strain, as detected by western blotting (A). p53R2, phospho-Akt, and Akt expression was observed in OA chondrocytes after p53R2 inhibition (B). Osteoarthritis and Cartilage 2012 20, 1603-1609DOI: (10.1016/j.joca.2012.08.022) Copyright © 2012 Osteoarthritis Research Society International Terms and Conditions