Enterotoxin A presence in lung AMs and dendritic cells.

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Enterotoxin A presence in lung AMs and dendritic cells. Enterotoxin A presence in lung AMs and dendritic cells. Lung tissue was harvested 1, 4, or 24 h after enterotoxin A inhalation or from vehicle control (1 or 4 h). AMs were identified as live CD45+CD11c+Siglec F+CD169+ cells and dendritic cells as live CD45+CD11c+Siglec F−MHC II+. (A) Relative median fluorescence intensity (MFI) of enterotoxin A on surface and intracellularly plus surface in lung AMs 1, 4, or 24 h after enterotoxin A inhalation. (B) Relative MFI of enterotoxin A on surface and intracellularly plus surface in lung dendritic cells 1, 4, or 24 h after enterotoxin A inhalation. MFI values are relative to the vehicle control (set as 1). Two independent experiments with a total of n = 4 per group were performed. Data are represented as mean ± SEM. One-way ANOVA with a Dunnett test was used: *p < 0.05, **p < 0.01, ***p < 0.001. Julia Svedova et al. ImmunoHorizons 2017;1:213-222 Copyright © 2017 The Authors