Volume 16, Issue 1, Pages (July 2014)

Slides:



Advertisements
Similar presentations
Cell-to-Cell Transfer of M. tuberculosis Antigens Optimizes CD4 T Cell Priming Smita Srivastava, Joel D. Ernst Cell Host & Microbe Volume 15, Issue 6,
Advertisements

The Quest for a Universal Flu Vaccine: Headless HA 2.0
Volume 10, Pages (August 2016) A Single Human Papillomavirus Vaccine Dose Improves B Cell Memory in Previously Infected Subjects  Erin M. Scherer,
Predicting Vaccine Responsiveness
Methodologic Considerations in the Application of Next-Generation Sequencing of Human TRB Repertoires for Clinical Use  Liwen Xu, Xiaoqing You, PingPing.
Persistence and evolution of allergen-specific IgE repertoires during subcutaneous specific immunotherapy  Mattias Levin, PhD, Jasmine J. King, BS, Jacob.
Peter D. Kwong, John R. Mascola  Immunity 
Anergic B Cells Caught in the Act
Prediction of IgE-binding epitopes by means of allergen surface comparison and correlation to cross-reactivity  Fabio Dall'Antonia, PhD, Anna Gieras,
Influenza Vaccines: Challenges and Solutions
Volume 29, Issue 3, Pages (September 2008)
Tiago R. Matos, Menno A. de Rie, Marcel B.M. Teunissen 
Convergent Antibody Signatures in Human Dengue
Volume 74, Issue 5, Pages (November 2018)
IgA and IgM VH repertoires in human colon: Evidence for clonally expanded B cells that are widely disseminated  Wolfgang Holtmeier, Andreas Hennemann,
Volume 20, Issue 4, Pages (October 2016)
Applications of Immunogenomics to Cancer
Accurate Sample Assignment in a Multiplexed, Ultrasensitive, High-Throughput Sequencing Assay for Minimal Residual Disease  Jack Bartram, Edward Mountjoy,
Autoreactivity in Human IgG+ Memory B Cells
Volume 12, Issue 4, Pages (October 2012)
Volume 18, Issue 3, Pages (September 2015)
Volume 15, Issue 6, Pages (June 2014)
Ramona A. Hoh, PhD, Shilpa A
Volume 38, Issue 2, Pages (February 2013)
Volume 4, Issue 1, Pages (January 1996)
Volume 16, Issue 5, Pages (November 2014)
Longitudinal analysis of cross-group HA stem–binding lineages.
Volume 16, Issue 2, Pages (August 2014)
Luisa De Sordi, Varun Khanna, Laurent Debarbieux  Cell Host & Microbe 
Antibody-encoding repertoires of bone marrow and peripheral blood—a focus on IgE  Mattias Levin, PhD, Fredrik Levander, PhD, Robert Palmason, MD, Lennart.
Volume 18, Issue 4, Pages (October 2015)
Germinal Center Selection and the Antibody Response to Influenza
Volume 19, Issue 6, Pages (June 2016)
Origin of Immunoglobulin Isotype Switching
Volume 48, Issue 2, Pages e5 (February 2018)
Functional Dissection of sRNA Translational Regulators by Nonhomologous Random Recombination and In Vivo Selection  Jane M. Liu, Joshua A. Bittker, Maria.
Volume 22, Issue 3, Pages e5 (September 2017)
Volume 18, Issue 5, Pages (November 2015)
Volume 153, Issue 4, Pages (May 2013)
A Serpin Takes a Bite out of the Flu
De novo oligoclonal expansions of circulating plasmablasts in active and relapsing IgG4- related disease  Hamid Mattoo, PhD, Vinay S. Mahajan, MBBS, PhD,
Volume 1, Issue 2, Pages (April 2007)
Andrew R. Bassett, Charlotte Tibbit, Chris P. Ponting, Ji-Long Liu 
Peter A. Savage, Mark M. Davis  Immunity 
Longitudinal analysis of ZIKV E–specific memory B cell responses in the DENV-experienced donors. Longitudinal analysis of ZIKV E–specific memory B cell.
Volume 13, Issue 12, Pages (December 2015)
Adam S. Dingens, Hugh K. Haddox, Julie Overbaugh, Jesse D. Bloom 
Autoreactivity in Human IgG+ Memory B Cells
Volume 22, Issue 2, Pages (August 2017)
Convergent Antibody Signatures in Human Dengue
Volume 16, Issue 3, Pages (September 2014)
High-throughput sequencing of the B-cell receptor in African Burkitt lymphoma reveals clues to pathogenesis by Katharine A. Lombardo, David G. Coffey,
Volume 13, Issue 3, Pages (March 2013)
A Neural Signature of Hierarchical Reinforcement Learning
Genome Sequences from Extinct Relatives
Recognizing Macrophage Activation and Host Defense
The Shaping of the T Cell Repertoire
Junctional Amino Acids Determine the Maturation Pathway of an Antibody
Vaccine Adjuvants Alter TCR-Based Selection Thresholds
Volume 5, Issue 5, Pages (May 2009)
Volume 14, Issue 4, Pages (October 2013)
Analysis of plasmablast responses in one naïve and three DENV-experienced donors. Analysis of plasmablast responses in one naïve and three DENV-experienced.
Volume 153, Issue 7, Pages (June 2013)
Volume 17, Issue 6, Pages (November 2016)
Broadening Horizons: New Antibodies Against Influenza
Antigen Selection of Anti-DSG1 Autoantibodies During and Before the Onset of Endemic Pemphigus Foliaceus  Ye Qian, Stephen H. Clarke, Valeria Aoki, Gunter.
IL‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response The diversity of.
Volume 15, Issue 6, Pages (June 2014)
Pneumococcus Adapts to the Sickle Cell Host
Volume 25, Issue 4, Pages e3 (October 2018)
Presentation transcript:

Volume 16, Issue 1, Pages 105-114 (July 2014) Human Responses to Influenza Vaccination Show Seroconversion Signatures and Convergent Antibody Rearrangements  Katherine J.L. Jackson, Yi Liu, Krishna M. Roskin, Jacob Glanville, Ramona A. Hoh, Katie Seo, Eleanor L. Marshall, Thaddeus C. Gurley, M. Anthony Moody, Barton F. Haynes, Emmanuel B. Walter, Hua-Xin Liao, Randy A. Albrecht, Adolfo García-Sastre, Javier Chaparro-Riggers, Arvind Rajpal, Jaume Pons, Birgitte B. Simen, Bozena Hanczaruk, Cornelia L. Dekker, Jonathan Laserson, Daphne Koller, Mark M. Davis, Andrew Z. Fire, Scott D. Boyd  Cell Host & Microbe  Volume 16, Issue 1, Pages 105-114 (July 2014) DOI: 10.1016/j.chom.2014.05.013 Copyright © 2014 Elsevier Inc. Terms and Conditions

Cell Host & Microbe 2014 16, 105-114DOI: (10.1016/j.chom.2014.05.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 1 Quantitation of Clonal B Cells in the Blood after Vaccination Predicts Seroconversion (A) Replicate IGH libraries were generated from peripheral-blood B cells for 14 individuals (Table S1) at three time points: prevaccination (red arc), day 7 postvaccination (green arc), and day 21 postvaccination (purple arc). Replicates are shown as bands within each arc, and lines connect clonally related VDJs from independent replicates. Detailed IGH repertoires for each individual are shown in Movie S1. Figure S1 presents criteria for the definition of clonal lineages. (B) Normalized clonality index scores; prevaccination (red) and on days 7 (green) and 21 (purple) postvaccination. (C) Plasmablast percentages of total B cells (Table S3); prevaccination (red) and on days 7 (green) and 21 (purple) postvaccination. (D) Correlation between metrics and serological antibody response. Cell Host & Microbe 2014 16, 105-114DOI: (10.1016/j.chom.2014.05.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 2 Antigen Specificity of Vaccine-Induced B Cell Clonal Populations (A) B cell lineages with members from mAbs derived from day 7 sorted plasmablasts (blue arc) and deep-sequenced IGH from peripheral B cells prior to (red) and on days 7 (green) and 21 (purple) after TIV vaccination. Lines join lineage members. (B) Antigen binding of plasmablast-derived mAbs (Table S2); influenza antigen (green), unknown or untested (yellow), noninfluenza antigen (red). Arcs are “zoomed” to shared lineages. 7014 had no shared lineages. Cell Host & Microbe 2014 16, 105-114DOI: (10.1016/j.chom.2014.05.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 3 Time Course of B Cell Clonal Expansions Induced by Inactivated H1N1 Vaccine (A) Vaccine-induced B cell clonal lineage relationships during H1N1 vaccine response (complete IGH repertoires are shown in Movie S2). Each arc represents a postvaccination time point subdivided into expanded (blue) and unexpanded (yellow) compartments. Lines join lineage members at later time points. Orange lines indicate that a lineage was strongly induced at the originating time point (>0.1% of total IGH). (B) IGHV mutation percentage for strongly induced lineages. Median (red line), quartiles shown as box and whiskers, and points are jittered for preventing overplotting. Cell Host & Microbe 2014 16, 105-114DOI: (10.1016/j.chom.2014.05.013) Copyright © 2014 Elsevier Inc. Terms and Conditions

Figure 4 Convergent IGH Rearrangements Identified in H1N1 Vaccine Responses in Different Individuals Multiple-sequence alignment of closely related stereotypic H1N1 clones isolated from difference sources. Nucleotide positions are labeled when they differ from the germline genes (top of each block). Nucleotides inferred to be derived from nontemplated nucleotide addition during V(D)J rearrangement are not colored. Amino acid positions are labeled when they differ from those in the mAb (bottom of each block). Each block is labeled with the germline, the conserved sequence group (CSG), and the mAb. Members of CSGs are detailed in Table S4. (A) IGH related to mAb 4K8 (Krause et al., 2011). (B) IGH related to mAb 2K11 (Krause et al., 2011). (C) IGH related to CDR3s previously identified by Wrammert et al. (2011). (D) The percentage of total sequences with CDR1 and CDR2 somatic amino acid substitutions in convergent H1N1 clones (upper) in comparison to 4,784 IGHs using IGHV3-7 from the prevaccination repertoire of 14 subjects prepandemic (lower). The germline sequence is depicted in the white boxes (middle). Asterisks mark amino acid substitutions shared with mAbs 48K or 2K11 (Krause et al., 2011). Cell Host & Microbe 2014 16, 105-114DOI: (10.1016/j.chom.2014.05.013) Copyright © 2014 Elsevier Inc. Terms and Conditions