Reassembling Biological Machinery In Vitro

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Reassembling Biological Machinery In Vitro Henry Hess Chemistry & Biology Volume 16, Issue 9, Pages 917-918 (September 2009) DOI: 10.1016/j.chembiol.2009.09.004 Copyright © 2009 Elsevier Ltd Terms and Conditions

Figure 1 Controlled Immobilization of Two Enzymes of the Glycolytic Cycle Two genetically engineered enzymes, hexokinase (HK) and glucose-6-phosphate isomerase (GPI), linked to a functionalized surface via His6-tag/Ni-NTA interactions, perform the first two steps of the glycolytic pathway conversion of glucose to glucose-6-phosphate catalyzed by HK, followed by subsequent transformation of glucose-6-phosphate to fructose-6-phosphate, catalyzed by GPI. Chemistry & Biology 2009 16, 917-918DOI: (10.1016/j.chembiol.2009.09.004) Copyright © 2009 Elsevier Ltd Terms and Conditions