sgSNP 377 Contig21552 sgSNP 247 Contig11105 sgSNP 1433 Contig10821

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sgSNP 377 Contig21552 sgSNP 247 Contig11105 sgSNP 1433 Contig10821 Reference reaction Reaction with allele 1 Reaction with allele 2 2.0 1.5 1.0 sgSNP 377 Contig21552 0.5 65.0 75.0 80.0 85.0 90.0 95.0 70.0 0.8 0.6 0.4 0.2 sgSNP 247 Contig11105 0.0 65.0 75.0 80.0 85.0 90.0 95.0 70.0 1.6 1.4 1.2 1.0 0.8 0.6 sgSNP 1433 Contig10821 0.4 0.2 65.0 70.0 75.0 80.0 85.0 90.0 95.0 1.5 1.3 1.1 0.9 0.7 sgSNP 193 Contig10284 0.5 0.3 0.1 65.0 70.0 75.0 80.0 85.0 90.0 95.0 1.6 1.4 1.2 1.0 0.8 0.6 0.4 sgSNP 521 Contig 17875 0.2 65.0 70.0 75.0 80.0 85.0 90.0 95.0 65.0 75.0 80.0 85.0 90.0 95.0 70.0 1.4 1.2 1.0 0.6 0.4 0.8 0.2 sgSNP 1297 Contig18072 Supplemental Figure S6. Amplification plot (left) and melting curve (right) of sgSNPs by qPCR. The amplification graphics show the ΔRn during the cycles reaction to different reactions (reference reaction, reaction with allele 1 and reaction with allele 2). The melting curve graphic shows the representation of derivative reporter (-Rn) during increase of temperature and the formation of only one peak showing that the reaction had specific amplification.