A 58-Year-Old Man with Respiratory Insufficiency After a 50-Year History of Hypersensitivity Pneumonitis and Pulmonary Aspergillus Infections Florian.

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A 58-Year-Old Man with Respiratory Insufficiency After a 50-Year History of Hypersensitivity Pneumonitis and Pulmonary Aspergillus Infections Florian Segerer, MD, Henner Morbach, MD, Nicole Hassold, MD, Stefan Kleinert, MD, Hans-Peter Tony, MD, Joachim Roesler, MD, Johannes Liese, MD, MSc The Journal of Allergy and Clinical Immunology: In Practice Volume 1, Issue 6, Pages 677-680 (November 2013) DOI: 10.1016/j.jaip.2013.09.001 Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Figure 1 Measurement of granulocyte intracellular production of ROS by dihydrorhodamine 123 FACS analysis (fluorescence-activated cell sorting). The oxidation of dihydrorhodamine 123 to rhodamine after stimulation with phorbol 12-myristate 13-acetate (PMA) is measured by an increase in fluorescence intensity. Granulocytes of the healthy control show an increase of fluorescence from 6 to 429, corresponding to a stimulation index of more than 40. The patient's granulocytes show a decreased stimulation index of 2, which indicated a severe reduction of ROS production. The Journal of Allergy and Clinical Immunology: In Practice 2013 1, 677-680DOI: (10.1016/j.jaip.2013.09.001) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Figure 2 Computed tomography of the thorax, showing granuloma, emphysema, intrapulmonary consolidations, abundant reticular scaring (white arrows), traction bronchiectasis, and bronchus dilatation (white asterisk). Status is shown after partial resection of the right and left upper lobe after recurrent pulmonary aspergillosis with residual pleural thickening. The Journal of Allergy and Clinical Immunology: In Practice 2013 1, 677-680DOI: (10.1016/j.jaip.2013.09.001) Copyright © 2013 American Academy of Allergy, Asthma & Immunology Terms and Conditions