Single layer centrifugation of stallion spermatozoa improves sperm quality compared with sperm washing  J.M. Morrell, H. Rodriguez-Martinez, A. Johannisson  Reproductive BioMedicine Online  Volume 21, Issue 3, Pages 429-436 (September 2010) DOI: 10.1016/j.rbmo.2010.03.027 Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Effect of single layer centrifugation and sperm washing on mean±SD of stallion sperm kinematics (n=18). Uncent, uncentrifuged sperm sample; SLC, single layer centrifugation; washed, centrifugation without a colloid; Day 1, 0h; Day 2, 24h; Day 3, 48h. For significant differences, see text. Reproductive BioMedicine Online 2010 21, 429-436DOI: (10.1016/j.rbmo.2010.03.027) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Effect of single layer centrifugation, seminal plasma (1.5%) and sperm washing on mean values±€ SD of stallion sperm kinematics (n=10). Uncent, uncentrifuged sperm sample; SLC, single layer centrifugation; washed, centrifugation without a colloid; SLC+SP, seminal plasma added to the selected spermatozoa after single layer centrifugation to give approximately the same ratio as in the washed sample (1.5%). Total motility and SLC were better than uncentrifuged and washed at all time points (P<0.001) and better than SLC+SP at 48h (P<0.05). Reproductive BioMedicine Online 2010 21, 429-436DOI: (10.1016/j.rbmo.2010.03.027) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Effect of different centrifugation treatments and presence of seminal plasma (1.5%) on DNA fragmentation index of stallion spermatozoa (n=10). Uncent, uncentrifuged sperm samples; SLC, single layer centrifugation; washed, centrifugation without a colloid; SLC+SP, seminal plasma added to the selected spermatozoa after single layer centrifugation to give approximately the same ratio as in the washed sample (1.5%). Significant differences: at 0h: Uncent versus SLC P<0.05, Uncent versus SLC+SP P<0.001, Uncent versus Washed NS, SLC versus SLC+SP NS, SLC versus Washed P<0.05, SLC+SP versus Washed P<0.001; at 24h: Uncent versus SLC P<0.001, Uncent versus SLC+SP P<0.001, Uncent versus Washed <0.05, SLC versus SLC+SP NS, SLC versus Washed P<0.01, SLC+SP versus Washed P<0.05. NS, not significant. Reproductive BioMedicine Online 2010 21, 429-436DOI: (10.1016/j.rbmo.2010.03.027) Copyright © 2010 Reproductive Healthcare Ltd. Terms and Conditions